Human TMS1 (PYCARD) activation kit by CRISPRa

CAT#: GA109241

PYCARD CRISPRa kit - CRISPR gene activation of human PYD and CARD domain containing

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: PYCARD
• Locus ID: 29108
• Kit Components:

  GA109241G1, TMS1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA109241G2, TMS1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA109241G3, TMS1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_013258, NM_145182, NM_145183
• UniProt ID: Q9ULZ3
• Synonyms: ASC; CARD5; TMS; TMS-1; TMS1
• Summary: This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]