BMAL1 (ARNTL) (NM_001297724) Human Untagged Clone

CAT#: SC336873

ARNTL (untagged) - Human aryl hydrocarbon receptor nuclear translocator-like (ARNTL), transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BMAL1
• Synonyms: bHLHe5; BMAL1; BMAL1c; JAP3; MOP3; PASD3; TIC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336873 representing NM_001297724.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATTAATATAGAAAGCATGGACACAGACAAAGATGACCCTCATGGAAGGTTAGAATATACAGAACAC
  CAAGGAAGGATAAAAAATGCAAGGGAAGCTCACAGTCAGATTGAAAAGCGGCGTCGGGATAAAATGAAC
  AGTTTTATAGATGAATTGGCTTCTTTGGTACCAACATGCAACGCAATGTCCAGGAAATTAGATAAACTT
  ACTGTGCTAAGGATGGCTGTTCAGCACATGAAAACATTAAGAGGTGCCACCAATCCATACACAGAAGCA
  AACTACAAACCAACTTTTCTATCAGACGATGAATTGAAACACCTCATTCTCAGGGCAGCAGATGGATTT
  TTGTTTGTCGTAGGATGTGACCGAGGGAAGATACTCTTTGTCTCAGAGTCTGTCTTCAAGATCCTCAAC
  TACAGCCAGAATGATCTGATTGGTCAGAGTTTGTTTGACTACCTGCATCCTAAAGATATTGCCAAAGTC
  AAGGAGCAGCTCTCCTCCTCTGACACCGCACCCCGGGAGCGGCTCATAGATGCAAAAACTGGACTTCCA
  GTTAAAACAGATATAACCCCTGGGCCATCTCGATTATGTTCTGGAGCACGACGTTCTTTCTTCTGTAGG
  ATGAAGTGTAACAGGCCTTCAGTAAAGGTTGAAGACAAGGACTTCCCCTCTACCTGCTCAAAGAAAAAA
  GCAGATCGAAAAAGCTTCTGCACAATCCACAGCACAGGCTATTTGAAAAGCTGGCCACCCACAAAGATG
  GGGCTGGATGAAGACAACGAACCAGACAATGAGGGGTGTAACCTCAGCTGCCTCGTCGCAATTGGACGA
  CTGCATTCTCATGTAGTTCCACAACCAGTGAACGGGGAAATCAGGGTGAAATCTATGGAATATGTTTCT
  CGGCACGCGATAGATGGAAAGTTTGTTTTTGTAGACCAGAGGGCAACAGCTATTTTGGCATATTTACCA
  CAAGAACTTCTAGGCACATCGTGTTATGAATATTTTCACCAAGATGACATAGGACATCTTGCAGAATGT
  CATAGGCAAGTTTTACAGACGAGAGAAAAAATTACAACTAATTGCTATAAATTTAAAATCAAAGATGGT
  TCTTTTATCACACTACGGAGTCGATGGTTCAGTTTCATGAACCCTTGGACCAAGGAAGTAGAATATATT
  GTCTCAACTAACACTGTTGTTTTAGCCAACGTCCTGGAAGGCGGGGACCCAACCTTCCCACAGCTCACA
  GCATCCCCCCACAGCATGGACAGCATGCTGCCCTCTGGAGAAGGTGGCCCAAAGAGGACCCACCCCACT
  GTTCCAGGGATTCCAGGGGGAACCCGGGCTGGGGCAGGAAAAATAGGCCGAATGATTGCTGAGGAAATC
  ATGGAAATCCACAGGATAAGAGGGTCATCGCCTTCTAGCTGTGGCTCCAGCCCATTGAACATCACGAGT
  ACGCCTCCCCCTGATGCCTCTTCTCCAGGAGGCAAGAAGATTTTAAATGGAGGGACTCCAGACATTCCT
  TCCAGTGGCCTACTATCAGGCCAGGCTCAGGAGAACCCAGGTTATCCATATTCTGATAGTTCTTCTATT
  CTTGGTGAGAACCCCCACATAGGTATAGACATGATTGACAACGACCAAGGATCAAGTAGTCCCAGTAAT
  GATGAGGCAGCAATGGCTGTCATCATGAGCCTCTTGGAAGCAGATGCTGGACTGGGTGGCCCTGTTGAC
  TTTAGTGACTTGCCATGGCCGCTGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001297724
• Insert Size: 1752 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001297724.1
• RefSeq Size: 2875 bp
• RefSeq ORF: 1752 bp
• Locus ID: 406
• UniProt ID: O00327
• Cytogenetics: 11p15.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• MW: 64.2 kDa
• Gene Summary: The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (6) differs in the 5' UTR and contains an alternate exon compared to variant 4. The resulting isoform (d) has a shorter and distinct N-terminus compared to isoform c. Variants 6, 17, 18, 19, 20, and 21 all encode the same isoform (d).