CDC25C (NM_001287582) Human Untagged Clone

CAT#: SC336325

CDC25C (untagged) - Human cell division cycle 25C (CDC25C), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC25C
• Synonyms: CDC25; PPP1R60
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC336325 representing NM_001287582.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGTCTACGGAACTCTTCTCATCCACAAGAGAGGAAGGAAGCTCTGGCTCAGGACCCAGTTTTAGGTCT
  AATCAAAGGAAAATGTTAAACCTGCTCCTGGAGAGAGACACTTCCTTTACCGTCTGTCCAGATGTCCCT
  AGAACTCCAGTGGGCAAATTTCTTGGTGATTCTGCAAACCTAAGCATTTTGTCTGGAGGAACCCCAAAA
  CGTTGCCTCGATCTTTCGAATCTTAGCAGTGGGGAGATAACTGCCACTCAGCTTACCACTTCTGCAGAC
  CTTGATGAAACTGGTCACCTGGATTCTTCAGGACTTCAGGAAGTGCATTTAGCTGGGATGAATCATGAC
  CAGCACCTAATGAAATGTAGCCCAGCACAGCTTCTTTGTAGCACTCCGAATGGTTTGGACCGTGGCCAT
  AGAAAGAGAGATGCAATGTGTAGTTCATCTGCAAATAAAGAAAATGACAATGGAAACTTGGTGGACAGT
  GAAATGAAATATTTGGGCAGTCCCATTACTACTGTTCCAAAATTGGATAAAAATCCAAACCTAGGAGAA
  GACCAGGCAGAAGAGATTTCAGATGAATTAATGGAGTTTTCCCTGAAAGATCAAGAAGCAAAGGTGAGC
  AGAAGTGGCCTATATCGCTCCCCGTCGATGCCAGAGAACTTGAACAGGCCAAGACTGAAGCAGGTGGAA
  AAATTCAAGGACAACACAATACCAGATAAAGTTAAAAAAAAGTATTTTTCTGGCCAAGGAAAGCTCAGG
  AAGGGCTTATGTTTAAAGAAGACAGTCTCTCTGTGTGACATTACTATCACTCAGATGCTGGAGGAAGAT
  TCTAACCAGGGGCACCTGATTGGTGATTTTTCCAAGGTATGTGCGCTGCCAACCGTGTCAGGGAAACAC
  CAAGATCTGAAGTATGTCAACCCAGAAACAGTGGCTGCCTTACTGTCGGGGAAGTTCCAGGGTCTGATT
  GAGAAGTTTTATGTCATTGATTGTCGCTATCCATATGAGTATCTGGGAGGACACATCCAGGGAGCCTTA
  AACTTATATAGTCAGGAAGAACTGTTTAACTTCTTTCTGAAGAAGCCCATCGTCCCTTTGGACACCCAG
  AAGAGAATAATCATCGTGTTCCACTGTGAATTCTCCTCAGAGAGGGGCCCCCGAATGTGCCGCTGTCTG
  CGTGAAGAGGACAGGTCTCTGAACCAGTATCCTGCATTGTACTACCCAGAGCTATATATCCTTAAAGGC
  GGCTACAGAGACTTCTTTCCAGAATATATGGAACTGTGTGAACCACAGAGCTACTGCCCTATGCATCAT
  CAGGACCACAAGACTGAGTTGCTGAGGTGTCGAAGCCAGAGCAAAGTGCAGGAAGGGGAGCGGCAGCTG
  CGGGAGCAGATTGCCCTTCTGGTGAAGGACATGAGCCCATGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001287582
• Insert Size: 1422 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287582.1
• RefSeq Size: 2187 bp
• RefSeq ORF: 1422 bp
• Locus ID: 995
• UniProt ID: P30307
• Cytogenetics: 5q31.2
• Protein Families: Druggable Genome, Phosphatase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
• MW: 53.4 kDa
• Gene Summary: This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1 and 3 encode the same protein (isoform a).