ALDH4A1 (NM_001161504) Human Untagged Clone

CAT#: SC327568

ALDH4A1 (untagged)-Human aldehyde dehydrogenase 4 family member A1 (ALDH4A1) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALDH4A1
• Synonyms: ALDH4; P5CD; P5CDh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC327568 representing NM_001161504.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAAGCCATCCCATGCGTGGTGGGGGATGAGGAGGTGTGGACGTCGGACGTGCAGTACCAAGTGTCG
  CCTTTTAACCATGGACATAAGGTGGCCAAGTTCTGTTATGCAGACAAGAGCCTGCTCAACAAAGCCATT
  GAGGCTGCCCTGGCTGCCCGGAAAGAGTGGGACCTGAAGCCTATTGCAGACCGGGCCCAGATCTTCCTG
  AAGGCGGCAGACATGCTGAGTGGGCCGCGCAGGGCTGAGATCCTCGCCAAGACCATGGTGGGACAGGGT
  AAGACCGTGATCCAAGCGGAGATTGACGCTGCAGCGGAACTCATCGACTTCTTCCGGTTCAATGCCAAG
  TATGCGGTGGAGCTGGAGGGGCAGCAGCCCATCAGCGTGCCCCCGAGCACCAACAGCACGGTGTACCGG
  GGTCTGGAGGGCTTCGTGGCGGCCATCTCGCCCTTTAACTTCACTGCAATCGGCGGCAACCTGGCGGGG
  GCACCGGCCCTGATGGGCAACGTGGTCCTATGGAAGCCCAGTGACACTGCCATGCTGGCCAGCTATGCT
  GTCTACCGCATCCTTCGGGAGGCTGGCCTGCCCCCCAACATCATCCAGTTTGTGCCAGCTGATGGGCCC
  CTATTTGGGGACACTGTCACCAGCTCAGAGCACCTCTGTGGCATCAACTTCACAGGCAGTGTGCCCACC
  TTCAAACACCTGTGGAAGCAGGTGGCCCAGAACCTGGACCGGTTCCACACCTTCCCACGCCTGGCTGGA
  GAGTGCGGCGGAAAGAACTTCCACTTCGTGCACCGCTCGGCCGACGTGGAGAGCGTGGTGAGCGGGACC
  CTCCGCTCAGCCTTCGAGTACGGTGGCCAGAAGTGTTCCGCGTGCTCGCGTCTCTACGTGCCGCACTCG
  CTGTGGCCGCAGATCAAAGGGCGGCTGCTGGAGGAGCACAGTCGGATCAAAGTGGGCGACCCTGCAGAG
  GATTTTGGGACCTTCTTCTCTGCAGTGATTGATGCCAAGTCCTTTGCCCGTATCAAGAAGTGGCTGGAG
  CACGCACGCTCCTCACCCAGCCTCACCATCCTGGCCGGGGGCAAGTGTGATGACTCCGTGGGCTACTTT
  GTGGAGCCCTGCATCGTGGAGAGCAAGGACCCTCAGGAGCCCATCATGAAGGAGGAGATCTTCGGGCCT
  GTACTGTCTGTGTACGTCTACCCGGATGACAAGTACAAGGAGACGCTGCAGCTGGTTGACAGCACCACC
  AGCTATGGCCTCACGGGGGCAGTGTTCTCCCAGGATAAGGACGTCGTGCAGGAGGCCACAAAGGTGCTG
  AGGAATGCTGCCGGCAACTTCTACATCAACGACAAGTCCACTGGCTCGATAGTGGGCCAGCAGCCCTTT
  GGGGGGGCCCGAGCCTCTGGAACCAATGACAAGCCAGGGGGCCCACACTACATCCTGCGCTGGACGTCG
  CCGCAGGTCATCAAGGAGACACATAAGCCCCTGGGGGACTGGAGCTACGCGTACATGCAGTGA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001161504
• Insert Size: 1512 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161504.1
• RefSeq Size: 3259 bp
• RefSeq ORF: 1512 bp
• Locus ID: 8659
• UniProt ID: P30038
• Cytogenetics: 1p36.13
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways
• MW: 55.1 kDa
• Gene Summary: This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2009]
  Transcript Variant: This variant (3) has an alternate 5' exon, compared to variant P5CDhL, resulting in a downstream AUG start codon. The resulting isoform (b) has a shorter N-terminus, compared to isoform a.