MOV10 (NM_001130079) Human Untagged Clone

CAT#: SC325383

MOV10 (untagged)-Human Mov10, Moloney leukemia virus 10, homolog (mouse) (MOV10), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MOV10
• Synonyms: fSAP113; gb110
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC325383 representing NM_001130079.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCCAGTAAGTTCAGCTGCCGGCAGCTCCGGGAGGCGGGCCAGTGTTTCGAGAGTTTCCTGGTCGTT
  CGGGGACTGGACATGGAGACAGATCGCGAGCGGCTGCGGACCATTTATAACCGCGACTTCAAGATCAGC
  TTTGGGACCCCCGCCCCTGGCTTCTCCTCCATGCTGTATGGAATGAAGATTGCAAATCTGGCCTACGTC
  ACCAAGACTCGGGTCAGGTTCTTCAGACTCGACCGCTGGGCCGACGTGCGGTTCCCAGAAAAGAGGAGA
  ATGAAGCTGGGGTCAGATATCAGCAAACACCACAAGTCACTGCTAGCCAAGATCTTTTATGACAGGGCT
  GAGTATCTTCATGGGAAACATGGTGTGGATGTGGAAGTCCAGGGGCCCCATGAAGCCCGAGATGGGCAG
  CTCCTTATCCGCCTGGATTTGAACCGCAAAGAGGTGCTGACCCTGAGGCTTCGGAATGGCGGAACCCAG
  TCTGTTACCCTCACTCACCTCTTCCCACTCTGCCGGACACCCCAGTTTGCTTTCTACAATGAAGACCAG
  GAGTTGCCCTGTCCACTGGGCCCCGGTGAATGCTATGAACTCCATGTCCATTGTAAGACCAGCTTTGTG
  GGCTACTTCCCAGCCACAGTGCTCTGGGAGCTGCTGGGACCTGGGGAGTCGGGTTCAGAAGGAGCCGGC
  ACATTCTACATTGCCCGCTTCTTGGCTGCCGTCGCCCACAGCCCCCTGGCTGCACAGCTGAAGCCCATG
  ACTCCCTTCAAGCGGACCCGGATCACCGGAAACCCTGTGGTGACCAATCGGATAGAGGAAGGAGAGAGA
  CCTGACCGCGCTAAGGGCTATGACCTGGAGTTAAGTATGGCGCTGGGGACATACTACCCACCTCCCCGC
  CTCAGGCAGCTGCTCCCCATGCTTCTTCAGGGAACAAGTATCTTCACTGCCCCTAAGGAGATCGCAGAG
  ATCAAGGCCCAGCTGGAGACAGCCCTGAAGTGGAGGAACTATGAGGTGAAGCTGCGGCTGCTGCTGCAC
  CTGGAGGAACTGCAGATGGAGCATGATATCCGGCACTATGACCTGGAGTCGGTGCCCATGACCTGGGAC
  CCTGTGGACCAGAACCCCAGGCTGCTCACGCTGGAGGTTCCTGGAGTGACTGAGAGCCGCCCCTCAGTG
  CTACGGGGCGACCACCTGTTTGCCCTTTTGTCCTCGGAGACACACCAGGAGGACCCCATCACATATAAG
  GGCTTTGTGCACAAGGTGGAATTGGACCGTGTCAAGCTGAGCTTTTCCATGAGCCTCCTGAGCCGCTTT
  GTGGATGGGCTGACCTTCAAGGTGAACTTTACCTTCAACCGCCAGCCGCTGCGAGTCCAGCACCGTGCC
  CTGGAGCTGACAGGGCGCTGGCTGCTGTGGCCCATGCTCTTTCCTGTGGCACCTCGGGACGTCCCGCTG
  CTGCCCTCAGATGTGAAACTCAAGCTGTACGACCGGAGTCTGGAGTCAAACCCAGAGCAGCTGCAGGCC
  ATGAGGCACATTGTTACGGGCACCACCCGTCCAGCCCCCTACATCATCTTTGGGCCTCCAGGCACCGGC
  AAGACTGTCACGTTAGTGGAGGCAATTAAGCAGGTGGTGAAGCACTTGCCCAAAGCCCACATCTTGGCC
  TGCGCTCCATCCAACTCAGGGGCTGACCTACTCTGTCAAAGGCTCCGGGTCCACCTTCCTAGCTCCATC
  TACCGCCTCCTGGCCCCCAGCAGGGACATCCGCATGGTACCTGAGGACATCAAGCCCTGCTGCAACTGG
  GACGCAAAGAAGGGGGAGTATGTATTTCCCGCCAAGAAGAAGCTGCAGGAATACCGGGTCTTAATTACC
  ACCCTCATCACTGCCGGCAGGTTGGTCTCGGCCCAGTTTCCCATTGATCACTTCACACACATCTTCATC
  GATGAGGCTGGCCACTGCATGGAGCCTGAGAGTCTGGTAGCTATAGCAGGGCTGATGGAAGTAAAGGAA
  ACAGGTGATCCAGGAGGGCAGCTGGTGCTGGCAGGAGACCCTCGGCAGCTGGGGCCTGTGCTGCGTTCC
  CCACTGACCCAGAAGCATGGACTGGGATACTCACTGCTGGAGCGGCTGCTCACCTACAACTCCCTGTAC
  AAGAAGGGCCCTGATGGCTATGACCCCCAGTTCATAACCAAGCTGCTCCGCAACTACAGGTCTCATCCC
  ACCATCCTGGACATTCCTAACCAGCTCTATTATGAAGGGGAGCTGCAGGCCTGTGCTGATGTCGTGGAT
  CGAGAACGCTTCTGCCGCTGGGCGGGCCTACCTCGACAGGGCTTTCCCATCATCTTTCACGGCGTAATG
  GGCAAAGATGAGCGTGAAGGCAACAGCCCATCCTTCTTCAACCCTGAAGAGGCTGCCACAGTGACTTCC
  TACCTGAAGCTGCTCCTGGCCCCCTCCTCCAAGAAGGGCAAAGCTCGCCTGAGCCCTCGAAGTGTGGGC
  GTCATCTCCCCGTACCGGAAACAGGTGGAGAAAATCCGTTACTGCATCACCAAACTTGACAGGGAGCTT
  CGAGGACTGGATGACATCAAGGACTTGAAGGTGGGTTCAGTAGAAGAATTCCAAGGCCAAGAACGAAGC
  GTCATCCTCATCTCCACCGTGCGAAGCAGCCAGAGCTTTGTGCAGCTGGATCTGGACTTTAATCTGGGT
  TTCCTTAAGAACCCCAAGAGGTTCAATGTAGCTGTGACCCGGGCCAAGGCCCTGCTCATCATCGTGGGG
  AACCCCCTTCTCCTGGGCCATGACCCTGACTGGAAAGTATTCCTGGAGTTCTGTAAAGAAAACGGAGGG
  TATACCGGGTGTCCCTTCCCTGCCAAACTGGACCTGCAACAGGGACAGAATTTACTGCAAGGTCTGAGC
  AAGCTCAGCCCCTCTACCTCAGGGCCCCACAGCCATGACTACCTCCCCCAGGAGCGGGAGGGTGAAGGG
  GGCCTGTCTCTGCAAGTGGAGCCAGAGTGGAGGAATGAGCTCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001130079
• Insert Size: 3012 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130079.1
• RefSeq Size: 3491 bp
• RefSeq ORF: 3012 bp
• Locus ID: 4343
• UniProt ID: Q9HCE1
• Cytogenetics: 1p13.2
• MW: 113.7 kDa
• Gene Summary: 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs (PubMed:24726324). Required for microRNA (miRNA)-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (PubMed:16289642, PubMed:17507929, PubMed:22791714). In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2 (PubMed:25464849). Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1 (PubMed:30122351, PubMed:23093941). Facilitates LINE-1 uridylation by TUT4 and TUT7 (PubMed:30122351). Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol (By similarity). May function as a messenger ribonucleoprotein (mRNP) clearance factor (PubMed:24726324).[UniProtKB/Swiss-Prot Function]