PAK5 (NM_177990) Human Untagged Clone

CAT#: SC323605

PAK7 (untagged)-Kinase deficient mutant (K478M) of Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAK5
• Synonyms: PAK7
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_177990, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTTGGGAAGAAAAAGAAAAAGATTGAAATATCTGGCCCGTCCAACTTTGAACACAGGGTTCATACTG
  GGTTTGATCCACAAGAGCAGAAGTTTACCGGCCTTCCCCAGCAGTGGCACAGCCTGTTAGCAGATACGGC
  CAACAGGCCAAAGCCTATGGTGGACCCTTCATGCATCACACCCATCCAGCTGGCTCCTATGAAGACAATC
  GTTAGAGGAAACAAACCCTGCAAGGAAACCTCCATCAACGGCCTGCTAGAGGATTTTGACAACATCTCGG
  TGACTCGCTCCAACTCCCTAAGGAAAGAAAGCCCACCCACCCCAGATCAGGGAGCCTCCAGCCACGGTCC
  AGGCCACGCGGAAGAAAATGGCTTCATCACCTTCTCCCAGTATTCCAGCGAATCCGATACTACTGCTGAC
  TACACGACCGAAAAGTACAGGGAGAAGAGTCTCTATGGAGATGATCTGGATCCGTATTATAGAGGCAGCC
  ACGCAGCCAAGCAAAATGGGCACGTAATGAAAATGAAGCACGGGGAGGCCTACTATTCTGAGGTGAAGCC
  TTTGAAATCCGATTTTGCCAGATTTTCTGCCGATTATCACTCACATTTGGACTCACTGAGCAAACCAAGT
  GAATACAGTGACCTCAAGTGGGAGTATCAGAGAGCCTCGAGTAGCTCCCCTCTGGATTATTCATTCCAAT
  TCACACCTTCTAGAACTGCAGGGACCAGCGGGTGCTCCAAGGAGAGCCTGGCGTACAGTGAAAGTGAATG
  GGGACCCAGCCTGGATGACTATGACAGGAGGCCAAAGTCTTCGTACCTGAATCAGACAAGCCCTCAGCCC
  ACCATGCGGCAGAGGTCCAGGTCAGGCTCGGGACTCCAGGAACCGATGATGCCATTTGGAGCAAGTGCAT
  TTAAAACCCATCCCCAAGGACACTCCTACAACTCCTACACCTACCCTCGCTTGTCCGAGCCCACAATGTG
  CATTCCAAAGGTGGATTACGATCGAGCACAGATGGTCCTCAGCCCTCCACTGTCAGGGTCTGACACCTAC
  CCCAGGGGCCCTGCCAAACTACCTCAAAGTCAAAGCAAATCGGGCTATTCCTCAAGCAGTCACCAGTACC
  CGTCTGGGTACCACAAAGCCACCTTGTACCATCACCCCTCCCTGCAGAGCAGTTCGCAGTACATCTCCAC
  GGCTTCCTACCTGAGCTCCCTCAGCCTCTCATCCAGCACCTACCCGCCGCCCAGCTGGGGCTCCTCCTCC
  GACCAGCAGCCCTCCAGGGTGTCCCATGAACAGTTTCGGGCGGCCCTGCAGCTGGTGGTCAGCCCAGGAG
  ACCCCAGGGAATACTTGGCCAACTTTATCAAAATCGGGGAAGGCTCAACCGGCATCGTATGCATCGCCAC
  CGAGAAACACACAGGGAAACAAGTTGCAGTGAAGAAAATGGACCTCCGGAAGCAACAGAGACGAGAACTG
  CTTTTCAATGAGGTCGTGATCATGCGGGATTACCACCATGACAATGTGGTTGACATGTACAGCAGCTACC
  TTGTCGGCGATGAGCTCTGGGTGGTCATGGAGTTTCTAGAAGGTGGTGCCTTGACAGACATTGTGACTCA
  CACCAGAATGAATGAAGAACAGATAGCTACTGTCTGCCTGTCAGTTCTGAGAGCTCTCTCCTACCTTCAT
  AACCAAGGAGTGATTCACAGGGACATAAAAAGTGACTCCATCCTCCTGACAAGCGATGGCCGGATAAAGT
  TGTCTGATTTTGGTTTCTGTGCTCAAGTTTCCAAAGAGGTGCCGAAGAGGAAATCATTGGTTGGCACTCC
  CTACTGGATGGCCCCTGAGGTGATTTCTAGGCTACCTTATGGGACAGAGGTGGACATCTGGTCCCTCGGG
  ATCATGGTGATAGAAATGATTGATGGCGAGCCCCCCTACTTCAATGAGCCTCCCCTCCAGGCGATGCGGA
  GGATCCGGGACAGTTTACCTCCAAGAGTGAAGGACCTACACAAGGTTTCTTCAGTGCTCCGGGGATTCCT
  AGACTTGATGTTGGTGAGGGAGCCCTCTCAGAGAGCAACAGCCCAGGAACTCCTCGGACATCCATTCTTA
  AAACTAGCAGGTCCACCGTCTTGCATCGTCCCCCTCATGAGACAATACAGGCATCACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_177990 unedited
  CCGCCTCGTTCCAGCAAAGGGCGGTAGGCGCTGTACGGTTGGGAGGTCTATATAAGCAGAGCTCGTTTAG
  TGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCCCTTGCC
  CCTACCCAGCCAGTAGTAGTTCCCCAGCGTGCGCCCGGGGAGACCGGGAACATGGCGCTGGGAGCGCTGT
  AGCAGCTGAGAAGGGGCTGAGGCACCGCCGCTTCGCTGACAGCCGGCCACCAGAAATACACTGTGGTGAA
  ATGCTTCCACCTCTTGCTAAAATGAACACTGAAAAAAAATGAAGAAGACTGACAGCCCCCACCGAAGAGT
  GTGGGGGAGTAGAAATACCCCCCTCCTTCTGGAGTCTTTATTTCATCCCCCGGCCATCATATAAGGGTTT
  TTGCATCATGGTTGGGAAAAAAAAAAAAAAATTGAATATCGGGCGCTCCAAATTGGAAAAGGGTTCACTG
  GGTTGTTCCAAAAACAAACTTTACCAGCCCTTACAGAAATGGCCACC
  
  Kinase domain sequence

  >SC323605 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CTTAWCAATCGGGGAGGCTCACCGGCATCGTATGCATCGCCACCGAGAAACACACAGGGAAACAAGTTGC
  AGTGATGAAAATGGACCTCCGGAAGCAACAGAGACGAGAATTGCTTTTCAATGAGGTCGTGATCATGCGG
  GATTACCACCATGACAATGTGGTTGACATGTACAACAGCTACCTTGTCGGCGATGAGCTCTGGGTGGTCA
  TGGAGTTTCTAGAAGGTGGTGCCTTGACAGACATTGTGACTCACA
  
• Restriction Sites: Please inquire
• ACCN: NM_177990
• Insert Size: 4840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177990.1, NP_817127.1
• RefSeq Size: 4506 bp
• RefSeq ORF: 2160 bp
• Locus ID: 57144
• UniProt ID: Q9P286
• Cytogenetics: 20p12.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. This kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an internal 5' UTR exon, as compared to variant 1. Both variants encode the same protein.