CDK5 (NM_004935) Human Untagged Clone

CAT#: SC323541

CDK5 (untagged)-Kinase deficient mutant (K33M) of Human cyclin-dependent kinase 5 (CDK5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDK5
• Synonyms: LIS7; PSSALRE
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323541 sequence for NM_004935 edited (data generated by NextGen Sequencing)
  ATGCAGAAATACGAGAAACTGGAAAAGATTGGGGAAGGCACCTACGGAACTGTGTTCAAG
  GCCAAAAACCGGGAGACTCATGAGATCGTGGCTCTGAWRCGGGTGAGGCTGGATGACGAT
  GATGAGGGTGTGCCGAGTTCCGCCCTCCGGGAGATCTGCCTACTCAAGGAGCTGAAGCAC
  AAGAACATCGTCAGGCTTCATGACGTCCTGCACAGCGACAAGAAGCTGACTTTGGTTTTT
  GAATTCTGTGACCAGGACCTGAAGAAGTATTTTGACAGTTGCAATGGTGACCTCGATCCT
  GAGATTGTAAAGTCATTCCTCTTCCAGCTACTAAAAGGGCTGGGATTCTGTCATAGCCGC
  AATGTGCTACACAGGGACCTGAAGCCCCAGAACCTGCTAATAAACAGGAATGGGGAGCTG
  AAATTGGCTGATTTTGGCCTGGCTCGAGCCTTTGGGATTCCCGTCCGCTGTTACTCAGCT
  GAGGTGGTCACACTGTGGTACCGCCCACCGGATGTCCTCTTTGGGGCCAAGCTGTACTCC
  ACGTCCATCGACATGTGGTCAGCCGGCTGCATCTTTGCAGAGCTGGCCAATGCTGGGCGG
  CCTCTTTTTCCCGGCAATGATGTCGATGACCAGTTGAAGAGGATCTTCCGACTGCTTGGG
  ACGCCCACCGAGGAGCAGTGGCCCTCTATGACCAAGCTGCCAGACTATAAGCCCTATCCG
  ATGTACCCGGCCACAACATCCCTGGTGAACGTCGTGCCCAAACTCAATGCCACAGGGAGG
  GATCTGCTGCAGAACCTTCTGAAGTGTAACCCTGTCCAGCGTATCTCAGCAGAAGAGGCC
  CTGCAGCACCCCTACTTCTCCGACTTCTGTCCGCCCTAG
  
  Clone variation with respect to NM_004935.3
  98 a=>w;99 a=>r;657 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004935 unedited
  CCGCCCGTCTCAGCACTGGGCGGTAGGCGCTGTACGGTGGGAGGTTCTATATAAGCAGAGCTCGTTTAGT
  GAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCAGGGGTCC
  CCGCGGCCGCCGCGATGCAGAAATACGAGAAACTGGAAAAGATTGGGGAAGGCACCTACGGAACTGTGTT
  CAAGGCCAAAAACCGGGAGACTCATGAGATCGTGGCTCTGATGCGGGTGAGGCTGGATGACGATGATGAG
  GGTGTGCCGAGTTCCGCCCTCCGGGAGATCTGCCTACTCAAGGAGCTGAAGCACAAGAACATCGTCAGGC
  TTCATGACGTCCTGCACAGCGACAAGAAGCTGACTTTGGTTTTTGAATTCTGTGACCAGGACCTGAGAGT
  ATTTTGACAGTTGCAATGGTGACCTCGATCCTGAGATTGTAAAGTCATTCCTCTTCCAGCTACTAAAGGC
  TGGATTCTGTCATAGCCGCATGGTGCTAACACAGGGACCTGAGCCCAGAACCTGCTAATAAAAGATGGGG
  GAGCTGAACTGCTGATTGCCTGCCTCGAGCTTGGATCCGTCGCGTGTACCTAGCTGAGGTGTCACACGTG
  GTTACCCGCCCCCCGGAGTGTCCTCTTTGGCAGCCGTGATTCACAGTCCACTGCACGTGGTCACCCCGGT
  CTGATACTTGGCGAGACTGGCATGTCGTGGCGCGCGCCATTCCCGCATATGATGTCCTGATAGCACCTTG
  TAGAAGGATCTCCGACATGCTGTGGGAACCCCACAGAGACTGGGCCTTAGCACACACTGTCGCAATATAG
  CCATCTCATGAGTCCGCGCACCATCTCCTGGTGAGACGTGGCCCACTACGTGTCCACGAGGAGTCGTCGC
  ACCTGAAGAGTAACTCTCGCATCCGAAAGCGTTGCCATTGCATTTGACCTGTACCGCAACGT
  
  Kinase domain sequence

  >SC323541 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCAGGGGTCCCCGCGGCC
  GCCGCGATGCAGAAATACGAGAAACTGGAAAAGATTGGGGAAGGCACCTACGGAACTGTGTTCAAGGCCA
  AAAACCGGGAGACTCATGAGATCGTGGCTCTGATGCGGGTGAGGC
  
• Restriction Sites: Please inquire
• ACCN: NM_004935
• Insert Size: 1200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004935.2, NP_004926.1
• RefSeq Size: 1143 bp
• RefSeq ORF: 879 bp
• Locus ID: 1020
• UniProt ID: Q00535
• Cytogenetics: 7q36.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Alzheimer's disease, Axon guidance
• Gene Summary: This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.