MAPKAP Kinase 3 (MAPKAPK3) (NM_004635) Human Untagged Clone

CAT#: SC323455

MAPKAPK3 (untagged)-Kinase deficient mutant (K73M) of Human mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAPKAP Kinase 3
• Synonyms: 3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MDPT3; MK-3; MK3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323455 sequence for NM_004635 edited (data generated by NextGen Sequencing)
  ATGGATGGTGAAACAGCAGAGGAGCAGGGGGGCCCTGTGCCCCCGCCAGTTGCACCCGGC
  GGACCCGGCTTGGGCGGTGCTCCGGGGGGGCGGCGGGAGCCCAAGAAGTACGCAGTGACC
  GACGACTACCAGTTGTCCAAGCAGGTGCTGGGCCTGGGTGTGAACGGCAAAGTGCTGGAG
  TGCTTCCATCGGCGCACTGGACAGAAGTGTGCCCTGATGCTCCTGTATGACAGCCCCAAG
  GCCCGGCAGGAGGTAGACCATCACTGGCAGGCTTCTGGCGGCCCCCATATTGTCTGCATC
  CTGGATGTGTATGAGAACATGCACCATGGCAAGCGCTGTCTCCTCATCATCATGGAATGC
  ATGGAAGGTGGTGAGTTGTTCAGCAGGATTCAGGAGCGTGGCGACCAGGCTTTCACTGAG
  AGAGAAGCTGCAGAGATAATGCGGGATATTGGCACTGCCATCCAGTTTCTGCACAGCCAT
  AACATTGCCCACCGAGATGTCAAGCCTGAAAACCTACTCTACACATCTAAGGAGAAAGAC
  GCAGTGCTTAAGCTCACCGATTTTGGCTTTGCTAAGGAGACCACCCAAAATGCCCTGCAG
  ACACCCTGCTATACTCCCTATTATGTGGCCCCTGAGGTCCTGGGTCCAGAGAAGTATGAC
  AAGTCATGTGACATGTGGTCCCTGGGTGTCATCATGTACATCCTCCTTTGTGGCTTCCCA
  CCCTTCTACTCCAACACGGGCCAGGCCATCTCCCCGGGGATGAAGAGGAGGATTCGCCTG
  GGCCAGTACGGCTTCCCCAATCCTGAGTGGTCAGAAGTCTCTGAGGATGCCAAGCAGCTG
  ATCCGCCTCCTGTTGAAGACAGACCCCACAGAGAGGCTGACCATCACTCAGTTCATGAAC
  CACCCCTGGATCAACCAATCGATGGTAGTGCCACAGACCCCACTCCACACGGCCCGAGTG
  CTGCAGGAGGACAAAGACCACTGGGACGAAGTCAAGGAGGAGATGACCAGTGCCTTGGCC
  ACTATGCGGGTAGACTACGACCAGGTGAAGATCAAGGACCTGAAGACCTCTAACAACCGG
  CTCCTCAACAAGAGGAGAAAAAAGCAGGCAGGCAGCTCCTCTGCCTCACAGGGCTGCAAC
  AACCAGTAG
  
  Clone variation with respect to NM_004635.4
  218 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004635 unedited
  ACGCCCGTCCAGCAACGGGCGGAGGCGTCACGGAGGGAGGACACACAAGAGAGCCCGTTAGCGAACCGTC
  AGAACACCGTAATACGACACACCACAGGGCGGCCGCGAACACGGCACCAGCACCCGCGACCGCCTCTCCC
  GCCCCTCGCCGGCACCTCAGCAAGGCGCCACTAGAAGCGCCAGGCTGGGGCCGCCTCTGAGCGCCCCGCG
  GGGGCCATGGATGGTGAAACAGCAGAGGAGCAGGGGGGCCCTGTGCCCCCGCCAGTCGCACCCGGCGGAC
  CCGGCTTGGGCGGTGCTCCGGGGGGGCGGCGGGGAGCCCAAGAAGTACGCAGTGACCGACGACTACCAGT
  TGTCCCAAGCAGGTGCTGGGGCCTGGGGTGTGAACGGCAAAGTGCTTGGAGTTGCTTTCCTTCGCCCCAC
  TGGGACAAAATTGTGCCCTGAATGCTTCTTGTTTAACACCCCCAAGGCCGGGCAGGAGGTAAACAATCAC
  TGCCGGGCTTTGGGCGGCCCCATTTTTGCTGCCTTCTGGAAGTTTTTGAAAAAACATGCCCATGGGCAGC
  CCCTGCTTCTTATCCTTATGGGATGGATGGAAAGGGGGGAATTTTGTCAACAAGATTCAAGGGCGTGGCC
  AACAGGCTTTTACTGAAAAAAAAACTGCAGAGATAATGCGGGAAATTGGCACTCTGCATCCCGTTTCGCA
  CAGCCATAAAAATGCCCACCCAAATGTGTAAGCCCTGAAAACCTACTCTTCCACATCTTAAGGAGAAAAA
  AAGCAGTGCTTAAGCCTCACCCGAATTTGGGCTTTTGCTAAGAGACCCACCAAATGCCCTGCAAACCCCC
  TGCTATACTCCTATATGTGCCCTGAGTTCTGGTCAGAAAGTTGACAGTCATGTGACATGTGTCCTTGTGT
  CACATGTACTCTCTGTGCTTCCACCTCTACTCACACGCCAGCACTCTCCCAG
  
  Kinase domain sequence

  >SC323455 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TAGCAGGTGCTGGGCCTGGGTKGACGGCAAAGTGCTGGAGTGCTTCCATCGGCGCACTGGACAGAAGTGT
  GCCCTGATGCTCCTGTATGACAGCCCCAAGGCCCGGCAGGAGGTAGACCATCACTGGCAGGCTTCTGGCG
  GCCCCCATATTGTCTGCATCCTGGATGTGTATGAGAACATGCACCATGGCAAGCGCTGTCTCCTCATCAT
  CATGGAATGCATGGAAGGTGGTGAGTTGTTCAGCAGGATTCAGGA
  
• Restriction Sites: Please inquire
• ACCN: NM_004635
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004635.3, NP_004626.1
• RefSeq Size: 2500 bp
• RefSeq ORF: 1149 bp
• Locus ID: 7867
• UniProt ID: Q16644
• Cytogenetics: 3p21.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway, VEGF signaling pathway
• Gene Summary: This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.