CK1 epsilon (CSNK1E) (NM_152221) Human Untagged Clone

CAT#: SC323364

CSNK1E (untagged)-Kinase deficient mutant (K38M) of Human casein kinase 1, epsilon (CSNK1E), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CK1 epsilon
• Synonyms: CKIe; CKIepsilon; HCKIE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_152221, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCTACGTGTGGGGAACAAGTACCGCCTGGGACGGAAGATCGGGAGCGGGTCCTTCGGAGATATCT
  ACCTGGGTGCCAACATCGCCTCTGGTGAGGAAGTCGCCATCAAGCTGGAGTGTGTGAAGACAAAGCACCC
  CCAGCTGCACATCGAGAGCAAGTTCTACAAGATGATGCAGGGTGGCGTGGGGATCCCGTCCATCAAGTGG
  TGCGGAGCTGAGGGCGACTACAACGTGATGGTCATGGAGCTGCTGGGGCCTAGCCTCGAGGACCTGTTCA
  ACTTCTGTTCCCGCAAATTCAGCCTCAAGACGGTGCTGCTCTTGGCCGACCAGATGATCAGCCGCATCGA
  GTATATCCACTCCAAGAACTTCATCCACCGGGACGTCAAGCCCGACAACTTCCTCATGGGGCTGGGGAAG
  AAGGGCAACCTGGTCTACATCATCGACTTCGGCCTGGCCAAGAAGTACCGGGACGCCCGCACCCACCAGC
  ACATTCCCTACCGGGAAAACAAGAACCTGACCGGCACGGCCCGCTACGCTTCCATCAACACGCACCTGGG
  CATTGAGCAAAGCCGTCGAGATGACCTGGAGAGCCTGGGCTACGTGCTCATGTACTTCAACCTGGGCTCC
  CTGCCCTGGCAGGGGCTCAAAGCAGCCACCAAGCGCCAGAAGTATGAACGGATCAGCGAGAAGAAGATGT
  CAACGCCCATCGAGGTCCTCTGCAAAGGCTATCCCTCCGAATTCTCAACATACCTCAACTTCTGCCGCTC
  CCTGCGGTTTGACGACAAGCCCGACTACTCTTACCTACGTCAGCTCTTCCGCAACCTCTTCCACCGGCAG
  GGCTTCTCCTATGACTACGTCTTTGACTGGAACATGCTGAAATTCGGTGCAGCCCGGAATCCCGAGGATG
  TGGACCGGGAGCGGCGAGAACACGAACGCGAGGAGAGGATGGGGCAGCTACGGGGGTCCGCGACCCGAGC
  CCTGCCCCCTGGCCCACCCACGGGGGCCACTGCCAACCGGCTCCGCAGTGCCGCCGAGCCCGTGGCTTCC
  ACGCCAGCCTCCCGCATCCAGCCGGCTGGCAATACTTCTCCCAGAGCGATCTCGCGGGTCGACCGGGAGA
  GGAAGGTGAGTATGAGGCTGCACAGGGGTGCGCCCGCCAACGTCTCCTCCTCAGACCTCACTGGGCGGCA
  AGAGGTCTCCCGGATCCCAGCCTCACAGACAAGTGTGCCATTTGACCATCTCGGGAAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_152221 unedited
  CCGCCCGTCTCAGCAAATGGGCGGTAGGCGTGTACGGTGTGGAGGTCTATATAAGCAGAGCTCGTTTAGT
  GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGAGAGGCTGGC
  GCGCCGGGCGGCTCCGCGAATCCTCCGGCATCCGCCCCGGCGGGCCGCCCCCGCCCGCGGCAGCCCCCCG
  AGCAGTGGCCCGGCATCGGCGCCTTCCCGGCGGGCAAGAGTGAGCCATGGAGCTACGTGTGGGGAACAAG
  TACCGCCTGGGACGGAAGATCGGGAGCGGGTCCTTCGGAGATATCTACCTGGGTGCCAACATCGCCTCTG
  GTGAGGGAAGTCGCCATCATGCTGGAGTGTGTGAAGACAAAGCACCCCCAGCTGCACATCGAGAGCAAGT
  TCTACAAGATGATGCAGGTGCGTGGGATCCCGTCCATCAAGTGTGCGGAGCTGAGGCGACTACAACGTGA
  TGGTCATGAGCTGCTGGGGCCTAGCTCCGAGACCTTGTTCACTTCTTGTTCCCGCAAATTCAGCCTCAGA
  ACGTGCTGCCTCTGCGAACAGAATGAATCAGCCGCATCCAGAGTATAATTCCATCCAGAACTTCATCACC
  GGACGTCAGCGAACACTTCCTATGGGCTTAAAAAGGGCCACCTTGCTCATCTACTGCATTTGCCTTGCCC
  AAAGATTACCGGAGACGCGCGACCCCCACAGCATTTCCTATACCGGGAAACAAGAACTTGAACCGCCGCG
  CCGTTACGTCTTCTACACGACCTTTGATTATGACACACGCCGCAATGATACCTGGAACCTGGTCCATGGC
  TCATGACTCTACCGGGTCTTGCGTCTGAGGGTCTCAGGCCAGGCCGAATGTACGGACTTCAGAGAAGTTG
  CCTGTCTTCAGAGATCTCCTGATACACACTGTTGTCAT
  
  Kinase domain sequence

  >SC323364 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSATGMGCATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAG
  AATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGAGAGGCTGGCGCGCCGGGCG
  GCTCCGCGAATCCTCCGGCATCCGCCCCGGCGGGCCGCCCCCGCCCGCGGCAGCCCCCCGAGCAGTGGCC
  CGGCATCGGCGCCTTCCCGGCGGGCAAGAGTGAGCCATGGAGCTA
  
• Restriction Sites: Please inquire
• ACCN: NM_152221
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152221.2, NP_689407.1
• RefSeq Size: 2820 bp
• RefSeq ORF: 1251 bp
• Locus ID: 1454
• UniProt ID: P49674
• Cytogenetics: 22q13.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Circadian rhythm - mammal, Hedgehog signaling pathway, Wnt signaling pathway
• Gene Summary: The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (1) represents the longer and predominant transcript. Both variants 1 and 2 encode the same protein.