PRKACB (NM_002731) Human Untagged Clone

CAT#: SC323349

PRKACB (untagged)-Kinase deficient mutant (K73M) of Human protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKACB
• Synonyms: CAFD2; PKA C-beta; PKACB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323349 sequence for NM_002731 edited (data generated by NextGen Sequencing)
  ATGGGGAACGCGGCGACCGCCAAGAAAGGCAGCGAGGTGGAGAGCGTGAAAGAGTTTCTA
  GCCAAAGCCAAAGAAGACTTTTTGAAAAAATGGGAGAATCCAACTCAGAATAATGCCGGA
  CTTGAAGATTTTGAAAGGAAAAAAACCCTTGGAACAGGTTCATTTGGAAGAGTCATGTTG
  GTAAAACACAAAGCCACTGAACAGTATTATGCCATGAWGATCTTAGATAAGCAGAAGGTT
  GTTAAACTGAAGCAAATAGAGCATACTTTGAATGAGAAAAGAATATTACAGGCAGTGAAT
  TTTCCTTTCCTTGTTCGACTGGAGTATGCTTTTAAGGATAATTCTAATTTATACATGGTT
  ATGGAATATGTCCCTGGGGGTGAAATGTTTTCACATCTAAGAAGAATTGGAAGGTTCAGT
  GAGCCCCATGCACGGTTCTATGCAGCTCAGATAGTGCTAACATTCGAGTACCTCCATTCA
  CTAGACCTCATCTACAGAGATCTAAAACCTGAAAATCTCTTAATTGACCATCAAGGCTAT
  ATCCAGGTCACAGACTTTGGGTTTGCCAAAAGAGTTAAAGGCAGAACTTGGACATTATGT
  GGAACTCCAGAGTATTTGGCTCCAGAAATAATTCTCAGCAAGGGCTACAATAAGGCAGTG
  GATTGGTGGGCATTAGGAGTGCTAATCTATGAAATGGCAGCTGGCTATCCCCCATTCTTT
  GCAGACCAACCAATTCAGATTTATGAAAAGATTGTTTCTGGAAAGGTCCGATTCCCATCC
  CACTTCAGTTCAGATCTCAAGGACCTTCTACGGAACCTGCTGCAGGTGGATTTGACCAAG
  AGATTTGGAAATCTAAAGAATGGTGTCAGTGATATAAAAACTCACAAGTGGTTTGCCACG
  ACAGATTGGATTGCTATTTACCAGAGGAAGGTTGAAGCTCCATTCATACCAAAGTTTAGA
  GGCTCTGGAGATACCAGCAACTTTGATGACTATGAAGAAGAAGATATCCGTGTCTCTATA
  ACAGAAAAATGTGCAAAAGAATTTGGTGAATTTTAA
  
  Clone variation with respect to NM_002731.2
  218 a=>w
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002731 unedited
  ACCGCCCGTCTCAGCAAAGGGCGGTAGGCGCTGTACGGTTGGGAGGTCTATATAAGCAGAGCTCGTTTAG
  TGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATATGAAGGA
  GGCTGGGATAACTAGCTTGAAAGAAATTCAGTCTAGTTATAGACATCTTTGGCATTAATCTGATGTTTAC
  TAGTGATATCTCATGCTAGGCAGTTATGCTTTGCTTCTAGGGGCTTCTCTTTTTAAAACAAAAGAAAGCT
  CTTTTCGTTTTCTGTGTGCTGCATGCTCCAGTGTGTGTGTTTACACCATCGGTTCTTCTCCCTCTAGGGG
  ATGGGCATAACTCCCTTTGCTGTTGGATTGTTATTTTTGAGCATATGTTTTTGGAAAGGTTGGTTTTCAT
  CATGGAGTTGCTTGCCTCCTCCTTCAGAAATATCTGATTCTTTGGTGAAAGGATTTTCTAGCCCAAAGCA
  AAGGAAGAACTTTTTGAAAAAATGGGGAAATCCACCTCAGAATAATTGCCCGGACTGGAGATTTGGAAAG
  GAAAAAACCTTGGAACAGGTTCATTTGGAAAATCCATGTGGTAACACCAGCCCCTGAACAGTATATGCCT
  AGAGTCTCTATAAACCAAGGTTGTAACCGGACCAAGCCCTGACACAGTATATGCCTGAGAGATCTAGAAA
  GCAAAGGTGTAATGGACCATAGCAACTGATGGAAGGAATTACAGGCTGGATCCTTCTTGTCACGGATGCT
  TAGGAATCATTACCGTTGAATTCCGGTGAGTTCCCAAATGAGTCTGGCTCGCGTTGCCTAAGTCATCCGT
  CCTTCGACCTCCTTG
  
  Kinase domain sequence

  >SC323349 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  ACKATTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCG
  TCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATATGAAGGAGGCTGGG
  ATAACTAGCTTGAAAGAAATTCAGTCTAGTTATAGACATCTTTGGCATTAATCTGATGTTTACTAGTGAT
  ATCTCATGCTAGGCAGTTATGCTTTGCTTCTAGGGGCTTCTCTTT
  
• Restriction Sites: Please inquire
• ACCN: NM_002731
• Insert Size: 4350 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002731.2, NP_002722.1
• RefSeq Size: 4529 bp
• RefSeq ORF: 1056 bp
• Locus ID: 5567
• UniProt ID: P22694
• Cytogenetics: 1p31.1
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Hedgehog signaling pathway, Insulin signaling pathway, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Prion diseases, Progesterone-mediated oocyte maturation, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection, Wnt signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the serine/threonine protein kinase family. The encoded protein is a catalytic subunit of cAMP (cyclic AMP)-dependent protein kinase, which mediates signalling though cAMP. cAMP signaling is important to a number of processes, including cell proliferaton and differentiation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (2) differs in the 5' exon structure, compared to variant 1. The encoded isoform (2, also known as Cbeta1) has a distinct N-terminus and is shorter than isoform 1.