PRKACB (NM_207578) Human Untagged Clone

CAT#: SC322379

PRKACB (untagged)-Human protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKACB
• Synonyms: CAFD2; PKA C-beta; PKACB
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322379 GTTCGCTGGAGCCCTTTCCTCAGACCCGGCCCGGTCTTCGCGCCCGGACTCCTGGCGCCA
  GCGCTAGGCGCACTCACCGCTCTGACGGGTGCAGACGCGGGAGTTGTCCCAGACTGTGGA
  GTGGCGGGCACGGCCCCAGCCCCCCTTCCCTTCCCTGACCCCTTCTTGCCATCGCCCCAG
  ACATGGGGAACGCGGCGACCGCCAAGAAAGGCAGCGAGGTGGAGAGCGTGAAAGAGTTTC
  TAGCCAAAGCCAAAGAAGACTTTTTGAAAAAATGGGAGAATCCAACTCAGAATAATGCCG
  GACTTGAAGATTTTGAAAGGAAAAAAACCCTTGGAACAGGTTCATTTGGAAGAGTCATGT
  TGGTAAAACACAAAGCCACTGAACAGTATTATGCCATGAAGATCTTAGATAAGCAGAAGG
  TTGTTAAACTGAAGCAAATAGAGCATACTTTGAATGAGAAAAGAATATTACAGGCAGTGA
  ATTTTCCTTTCCTTGTTCGACTGGAGTATGCTTTTAAGGATAATTCTAATTTATACATGG
  TTATGGAATATGTCCCTGGGGGTGAAATGTTTTCACATCTAAGAAGAATTGGAAGGTTCA
  GTGAGCCCCATGCACGGTTCTATGCAGCTCAGATAGTGCTAACATTCGAGTACCTCCATT
  CACTAGACCTCATCTACAGAGATCTAAAACCTGAAAATCTCTTAATTGACCATCAAGGCT
  ATATCCAGGTCACAGACTTTGGGTTTGCCAAAAGAGTTAAAGGCAGAACTTGGACATTAT
  GTGGAACTCCAGAGTATTTGGCTCCAGAAATAATTCTCAGCAAGGGCTACAATAAGGCAG
  TGGATTGGTGGGCATTAGGAGTGCTAATCTATGAAATGGCAGCTGGCTATCCCCCATTCT
  TTGCAGACCAACCAATTCAGATTTATGAAAAGATTGTTTCTGGAAAGAACTTTTGATATG
  AACAAAACAAAACTTTGAGAAAAATTAACAGACAAGGCAGTGATTTATTTTTGAAGAATT
  TGAGAAGTGTAGACTCTCAAGAGGACTAAAGGTCATATGAAGAATGATGAGAGAACCAAA
  ATACATTAAAATCACAAATGGAAGAAGAATATTTTACTAATACAAAAACTAAGAATGTAA
  ATGTTATAATAATTGTTTCAAATCATTTAATTGACAGTAATTATAAAGTTCTTGAATCTT
  TACTATATTACTTTTATTTATACTTCATATAAGAAATCCAGTTTTCTAACAAGGATACTG
  TCATAACTAAATTTACATTTATTAAGAAAAACTGCTTTAGTTAAAATTAATGTGTCTTCA
  TTTTTATGCATTGGCCTCGATTTGCCAATCATTCTCTATTGGTTAAAATTTATATTCAGC
  TGTTTATGAATATATATTCATTTTATATCAAACTTTAAAATTTTGTATCTAATAATCAGC
  ATATATTCTAAAATCATAACAGTCTAAATCCTGGGCACCTTAGAAGAATGACACCAGAAA
  ACCTTATTATATCACAATATTCTGTTTTCCCCTTCATTTATTTAGAAATATGACAGGATA
  TTTGGTGTACTTTTGTTTTTTAACTAAAAGTACCAGATTCTCTCTCCCCATGTGGGATAT
  AAAATTATCCCCATCTCTTACTCCCTTTACTCATCTAAAGTAGAAGTCATGAAAGTGGAA
  TTTTTGCCATTAAAAGGCTCTGTATTATGTGAAGTTAGATTGTATTAACCATTTCCCAAT
  AAATCATCTGTTTCAAAACTCAAATTCAAACTAGAATGTGTCTCTATTCACATTGCAAAA
  ATATTATTGTCTCTCTGGTTAGTGGCTAAAAGCCAAATTGGAAACTAACTAGTTTTTTAA
  ATTTTTTAAATTGTGCAAATTATTAAAAATCCAATTTGGTCTTATAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_207578
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207578.1, NP_997461.1
• RefSeq Size: 1863 bp
• RefSeq ORF: 774 bp
• Locus ID: 5567
• UniProt ID: P22694
• Cytogenetics: 1p31.1
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Hedgehog signaling pathway, Insulin signaling pathway, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Prion diseases, Progesterone-mediated oocyte maturation, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection, Wnt signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the serine/threonine protein kinase family. The encoded protein is a catalytic subunit of cAMP (cyclic AMP)-dependent protein kinase, which mediates signalling though cAMP. cAMP signaling is important to a number of processes, including cell proliferaton and differentiation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (3) differs in both the 5' and 3' exon structures, compared to variant 1. The encoded isoform (3) has distinct N- and C-termini and is shorter than isoform 1.