SHMT1 (NM_004169) Human Untagged Clone

CAT#: SC321518

SHMT1 (untagged)-Human serine hydroxymethyltransferase 1 (soluble) (SHMT1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SHMT1
• Synonyms: CSHMT; SHMT
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004169.3 CAGGCACCGGCGGCGGCGTAGGACGGAGGCGTCGCTAGGCAGCTTCGAACCAGTGCAATG
  ACGATGCCAGTCAACGGGGCCCACAAGGATGCTGACCTGTGGTCCTCACATGACAAGATG
  CTGGCACAACCCCTCAAAGACAGTGATGTTGAGGTTTACAACATCATTAAGAAGGAGAGT
  AACCGGCAGAGGGTTGGATTGGAGCTGATTGCCTCGGAGAATTTCGCCAGCCGAGCAGTT
  TTGGAGGCCCTAGGCTCTTGCTTAAATAACAAATACTCTGAGGGGTACCCGGGCCAGAGA
  TACTATGGCGGGACTGAGTTTATTGATGAACTGGAGACCCTCTGTCAGAAGCGAGCCCTG
  CAGGCCTATAAGCTGGACCCACAGTGCTGGGGGGTCAACGTCCAGCCCTACTCAGGCTCC
  CCTGCAAACTTTGCTGTGTACACTGCCCTGGTGGAACCCCATGGGCGCATCATGGGCCTG
  GACCTTCCGGATGGGGGCCACCTGACCCATGGGTTCATGACAGACAAGAAGAAAATCTCT
  GCCACGTCCATCTTCTTTGAATCTATGCCCTACAAGGTGAACCCAGATACTGGCTACATC
  AACTATGACCAGCTGGAGGAGAACGCACGCCTCTTCCACCCGAAGCTGATCATCGCAGGA
  ACCAGCTGCTACTCCCGAAACCTGGAATATGCCCGGCTACGGAAGATTGCAGATGAGAAC
  GGGGCGTATCTCATGGCGGACATGGCTCACATCAGCGGGCTGGTGGCGGCTGGCGTGGTG
  CCCTCCCCATTTGAACACTGCCATGTGGTGACCACCACCACTCACAAGACCCTGCGAGGC
  TGCCGAGCTGGCATGATCTTCTACAGGAAAGGAGTGAAAAGTGTGGATCCCAAGACTGGC
  AAAGAGATTCTGTACAACCTGGAGTCTCTTATCAATTCTGCTGTGTTCCCTGGCCTGCAG
  GGAGGTCCCCACAACCACGCCATTGCTGGGGTTGCTGTGGCACTGAAGCAAGCTATGACT
  CTGGAATTTAAAGTTTATCAACACCAGGTGGTGGCCAACTGCAGGGCTCTGTCTGAGGCC
  CTGACGGAGCTGGGCTACAAAATAGTCACAGGTGGTTCTGACAACCATTTGATCCTTGTG
  GATCTCCGTTCCAAAGGCACAGATGGTGGAAGGGCTGAGAAGGTGCTAGAAGCCTGTTCT
  ATTGCCTGCAACAAGAACACCTGTCCAGGTGACAGAAGCGCTCTGCGGCCCAGTGGACTG
  CGGCTGGGGACCCCAGCACTGACGTCCCGTGGACTTTTGGAAAAAGACTTCCAAAAAGTA
  GCCCACTTTATTCACAGAGGGATAGAGCTGACCCTGCAGATCCAGAGCGACACTGGTGTC
  AGAGCCACCCTGAAAGAGTTCAAGGAGAGACTGGCAGGGGATAAGTACCAGGCGGCCGTG
  CAGGCTCTCCGGGAGGAGGTTGAGAGCTTCGCCTCTTTCTTCCCTCTGCCTGGCCTGCCT
  GACTTCTAAAGGAGCGGGCCCACTCTGGACCCACCTGGCGCCACAGAGGAAGCTGGCTGC
  CGGAGGACCCCCACTTGAGAGATGGATGAGCTGCTCCAAAGGGGAACTGTTGACACTCGG
  GCCCTTTGAGGGGGTTTCTTTTGGACTTTTTTCATGTTTTCTTCACAAATCAAAATTTGT
  TTAAGTCTCATTGTTAGTAATTCTGGGACAGGTTATTAAAGGATTTAAATTTGAACCTGG
  CTTTCTCACAGCTGGACATAATTCTAGGAAAATAAGATACTATGTCGCCACTTGGTCATA
  ATCATTTAGATGGTGGTGTAGGGCAAAGCTGTTAGAAAGATTGTAGCGTTTTACTCTCCC
  TGGGCTTTCCTCCGCCTTGCTGCAACAGAGAGGAAATGCCCATGTCCACAGCTTGTACAC
  ACTGCCCCCTCACTATCTTGTTATCCAGTGGCATGCCAAAGGAGAACTGAATTAGCTTCT
  GAGGCTTCTGCTGTAAATCAGAAGTGTATGTTAGTCAAGAGTAAACAAGATGCACCCAGT
  ATGGTGGGAGGGTTTTGCTGTCAGTAGCTCAAAGTATGGTGTAGAAATGGCCTCCTCCCT
  CCATCCTGGGAAGTCCCAGTCCCATCCTGGTGTGAGAATCAACCAGGCTTTCCTGCTCCA
  CCTGAGATAACCAACTCCCTCCCGTAATCAGGAAGCCAAATGTCACCTTCCCAAAGAAAT
  TTTATTTTCACGTAGCTGAAGTGCAAAACATAGATGACCATTTTTAATAAGCACAATCAA
  ATTTTTAACCACAGAATGTCTACAAGAATTATAGCTTTAAAAAATACAACCAATTTTTAT
  ATTTCAAAAATATTTGAACTCAAATAAATTAATTTCTTAAAAAGTAAAAAAAAAAAAAAA
  AAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004169
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004169.3, NP_004160.3
• RefSeq Size: 2540 bp
• RefSeq ORF: 1452 bp
• Locus ID: 6470
• UniProt ID: P34896
• Cytogenetics: 17p11.2
• Domains: SHMT
• Protein Pathways: Cyanoamino acid metabolism, Glycine, serine and threonine metabolism, Metabolic pathways, Methane metabolism, One carbon pool by folate
• Gene Summary: This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).