FADS1 (NM_013402) Human Untagged Clone

CAT#: SC320773

FADS1 (untagged)-Human fatty acid desaturase 1 (FADS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FADS1
• Synonyms: D5D; FADS6; FADSD5; LLCDL1; TU12
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_013402.3 GCGCGGCGTCGCCAGGCCAGCTATGGCCCCCGACCCGGTGGCCGCCGAGACCGCGGCTCA
  GGGACCTACCCCGCGCTACTTCACCTGGGACGAGGTGGCCCAGCGCTCAGGGTGCGAGGA
  GCGGTGGCTAGTGATCGACCGTAAGGTGTACAACATCAGCGAGTTCACCCGCCGGCATCC
  AGGGGGCTCCCGGGTCATCAGCCACTACGCCGGGCAGGATGCCACGGATCCCTTTGTGGC
  CTTCCACATCAACAAGGGCCTTGTGAAGAAGTATATGAACTCTCTCCTGATTGGAGAACT
  GTCTCCAGAGCAGCCCAGCTTTGAGCCCACCAAGAATAAAGAGCTGACAGATGAGTTCCG
  GGAGCTGCGGGCCACAGTGGAGCGGATGGGGCTCATGAAGGCCAACCATGTCTTCTTCCT
  GCTGTACCTGCTGCACATCTTGCTGCTGGATGGTGCAGCCTGGCTCACCCTTTGGGTCTT
  TGGGACGTCCTTTTTGCCCTTCCTCCTCTGTGCGGTGCTGCTCAGTGCAGTTCAGGCCCA
  GGCTGGCTGGCTGCAGCATGACTTTGGGCACCTGTCGGTCTTCAGCACCTCAAAGTGGAA
  CCATCTGCTACATCATTTTGTGATTGGCCACCTGAAGGGGGCCCCCGCCAGTTGGTGGAA
  CCACATGCACTTCCAGCACCATGCCAAGCCCAACTGCTTCCGCAAAGACCCAGACATCAA
  CATGCATCCCTTCTTCTTTGCCTTGGGGAAGATCCTCTCTGTGGAGCTTGGGAAACAGAA
  GAAAAAATATATGCCGTACAACCACCAGCACAAATACTTCTTCCTAATTGGGCCCTCAGC
  CTTGCTGCCTCTCTACTTCCAGTGGTATATTTTCTATTTTGTTATCCAGCGAAAGAAGTG
  GGTGGACTTGGCCTGGATGATTACCTTCTACGTCCGCTTCTTCCTCACTTATGTGCCACT
  ATTGGGGCTGAAAGCCTTCCTGGGCCTTTTCTTCATAGTCAGGTTCCTGGAAAGCAACTG
  GTTTGTGTGGGTGACACAGATGAACCATATTCCCATGCACATTGATCATGACCGGAACAT
  GGACTGGGTTTCCACCCAGCTCCAGGCCACATGCAATGTCCACAAGTCTGCCTTCAATGA
  CTGGTTCAGTGGACACCTCAACTTCCAGATTGAGCACCATCTTTTTCCCACGATGCCTCG
  ACACAATTACCACAAAGTGGCTCCCCTGGTGCAGTCCTTGTGTGCCAAGCATGGCATAGA
  GTACCAGTCCAAGCCCCTGCTGTCAGCCTTCGCCGACATCATCCACTCACTAAAGGAGTC
  AGGGCAGCTCTGGCTAGATGCCTATCTTCACCAATAACAACAGCCACCCTGCCCAGTCTG
  GAAGAAGAGGAGGAAGACTCTGGAGCCAAGGCAGAGGGGAGCTTGAGGGACAATGCCACT
  ATAGTTTAATACTCAGAGGGGGTTGGGTTTGGGGACATAAAGCCTCTGACTCAAACTCCT
  CCCTTTTATCTTCTAGCCACAGTTCTAAGACCCAAAGTGGGGGGTGGACACAGAAGTCCC
  TAGGAGGGAAGGAGCTGTTGGGGCAGGGGTGTAAATTATTTCCTTTTTCTAGTTTGGCAC
  ATGCAGGTAGTTGGTGAACAGAGAGAACCAGGAGGGTAACAGAAGAGGAGGGACCTACTG
  AACCCAGAGTCAGGAAGAGATTTAACACTAAAATTCCACTCATGCCGGGCGTGGTGGCAC
  GCGCCTGTAATCCCAGCTACCCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCGGGGAGG
  TGGAGGTTGCAGTGAGCTGAGATCACGCCATTGTACTCCAGCCTGGGCGACAGAGCAAGA
  CTCCATTTCAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_013402
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013402.3, NP_037534.2
• RefSeq Size: 4432 bp
• RefSeq ORF: 1335 bp
• Locus ID: 3992
• UniProt ID: O60427
• Cytogenetics: 11q12.2
• Domains: heme_1, FA_desaturase
• Protein Families: Transcription Factors, Transmembrane
• Protein Pathways: Biosynthesis of unsaturated fatty acids
• Gene Summary: The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008]