FADS1 (NM_013402) Human Untagged Clone

CAT#: SC127259

FADS1 (untagged)-Human fatty acid desaturase 1 (FADS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FADS1
• Synonyms: D5D; FADS6; FADSD5; LLCDL1; TU12
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013402, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGAACGCGCGCTGCGAGGCCCGCCGGTCTGCCCTGCGGTGCTGAAAACCCGGCGCGCAGGCGGCTGG
  CTCTGGGCGCGCGCCAGCAAATCCACTCCTGGAGCCCGCGGACCCCGAGCACGCGCCTGACAGCCCCTGC
  TGGCCCGGCGCGCGGCGTCGCCAGGCCAGCTATGGCCCCCGACCCGGTGGCCGCCGAGACCGCGGCTCAG
  GGACCTACCCCGCGCTACTTCACCTGGGACGAGGTGGCCCAGCGCTCAGGGTGCGAGGAGCGGTGGCTAG
  TGATCGACCGTAAGGTGTACAACATCAGCGAGTTCACCCGCCGGCATCCAGGGGGCTCCCGGGTCATCAG
  CCACTACGCCGGGCAGGATGCCACGGATCCCTTTGTGGCCTTCCACATCAACAAGGGCCTTGTGAAGAAG
  TATATGAACTCTCTCCTGATTGGAGAACTGTCTCCAGAGCAGCCCAGCTTTGAGCCCACCAAGAATAAAG
  AGCTGACAGATGAGTTCCGGGAGCTGCGGGCCACAGTGGAGCGGATGGGGCTCATGAAGGCCAACCATGT
  CTTCTTCCTGCTGTACCTGCTGCACATCTTGCTGCTGGATGGTGCAGCCTGGCTCACCCTTTGGGTCTTT
  GGGACGTCCTTTTTGCCCTTCCTCCTCTGTGCGGTGCTGCTCAGTGCAGTTCAGGCCCAGGCTGGCTGGC
  TGCAGCATGACTTTGGGCACCTGTCGGTCTTCAGCACCTCAAAGTGGAACCATCTGCTACATCATTTTGT
  GATTGGCCACCTGAAGGGGGCCCCCGCCAGTTGGTGGAACCACATGCACTTCCAGCACCATGCCAAGCCC
  AACTGCTTCCGCAAAGACCCAGACATCAACATGCATCCCTTCTTCTTTGCCTTGGGGAAGATCCTCTCTG
  TGGAGCTTGGGAAACAGAAGAAAAAATATATGCCGTACAACCACCAGCACAAATACTTCTTCCTAATTGG
  GCCCCCAGCCTTGCTGCCTCTCTACTTCCAGTGGTATATTTTCTATTTTGTTATCCAGCGAAAGAAGTGG
  GTGGACTTGGCCTGGATGATTACCTTCTACGTCCGCTTCTTCCTCACTTATGTGCCACTATTGGGGCTGA
  AAGCCTTCCTGGGCCTTTTCTTCATAGTCAGGTTCCTGGAAAGCAACTGGTTTGTGTGGGTGACACAGAT
  GAACCATATTCCCATGCACATTGATCATGACCGGAACATGGACTGGGTTTCCACCCAGCTCCAGGCCACA
  TGCAATGTCCACAAGTCTGCCTTCAATGACTGGTTCAGTGGACACCTCAACTTCCAGATTGAGCACCATC
  TTTTTCCCACGATGCCTCGACACAATTACCACAAAGTGGCTCCCCTGGTGCAGTCCTTGTGTGCCAAGCA
  TGGCATAGAGTACCAGTCCAAGCCCCTGCTGTCAGCCTTCGCCGACATCATCCACTCACTAAAGGAGTCA
  GGGCAGCTCTGGCTAGATGCCTATCTTCACCAATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013402 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCTGAAAACCCGGCG
  CGCAGGCGGCTGGCTCTGGGCGCGCGCCAGCAAATCCACTCCTGGAGCCCGCGGACCCCG
  AGCACGCGCCTGACAGCCCCTGCTGGCCCGGCGCGCGGCGTCGCCAGGCCAGCTATGGCC
  CCCGACCCGGTGGCCGCCGAGACCGCGGCTCAGGGACCTACCCCGCGCTACTTCACCTGG
  GACGAGGTGGCCCAGCGCTCAGGGTGCGAGGAGCGGTGGCTAGTGATCGACCGTAAGGTG
  TACAACATCAGCGAGTTCACCCGCCGGCATCCAGGGGGCTCCCGGGTCATCAGCCACTAC
  GCCGGGCAGGATGCCACGGATCCCTTTGTGGCCTTCCACATCAACAAGGGCCTTGTGAAG
  AAGTATATGAACTCTCTCCTGATTGGAGAACTGTCTCCAGAGCAGCCCAGCTTTGAGCCC
  ACCAAGAATAAAGAGCTGACAGATGAGTTCCGGGAGCTGCGGGCCACAGTGGAGCGGATG
  GGGCTCATGAAGGCCAACCATGTCTTCTTCCTGCTGTACCTGCTGCACATCTTGCTGCTG
  GATGGTGCAGCCTGGCTCACCCTTTGGGTCTTTGGGACGTCCTTTTTGCCCTTCCTCCTC
  TGTGCGGTGCTGCTCAGTGCAGTTCAAGCCCAGGCTGGCTGGCTGCAGCATGACTTTGGG
  CACCTGTCGGTCTTCAGCACCTCAAAGTGGAACCATCTGCTACATCATTTTGTGATTGGC
  CCCTGNAAGGGGCCCCGCCAGTTGGTGGAACCCATGCACTTCAGCACCATGCCAGCCCAC
  TGCTTCGCAAGACCAGAATAAATGCATCCTCTCTTGCTGGGGAAGATCTCTTGNNGAGCT
  GGAAAGAGATAATTTGCGTCACCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013402 unedited
  CCGCGGCCCGCAATTCTANATCGAGTTTTTTTTTTTTTTTTTTGAAAGGAGTCTTGCTCT
  GTCGCCCAGGCTGGAGTACAATGGCGTGATCTCAGCTCACTGCAACCTCCACCTCCCCGG
  TTCAAGCGATTCTCCTGCCTCAGCCTCCTGGGTAGCTGGGATTACAGGCGCGTGCCACCA
  CGCCCGGCATGAGTGGAATTTTAGTGTTAAATCTCTTACTGACTCTGGGTTCAGTAGGAC
  CCTCCTCTTCTGTTACCCTCCTGGTTCTCTCTGTTCACCAACTACCTGCATGTGCCAAAC
  TAGAAAAAGGAAATAATTTACACCCCTGCCCCAACAGCTCCTTCCCTCCTAGGGACTTCT
  GTGTCCACCCCCCACTTTGGGTCTTAAAACTGTGGCTACAAGATAAAAGGGAGGAGTTTG
  AGTCAGAGGCTTTATGTCCCCAAACCCAACCCCCTCTGAGTATTAAACTATAGTGGCATT
  GACCCTCAAGCTCCCCTCTGCCTTGGCTCCAGAGTCTTCCTACTCTTTTTACACACTGGG
  CAGGGCGGCTGCCGTATTGGTGAAGATAGGCATTCTACTCCGAGCTGCCCTGACTCCTTT
  ACCGAGTGGATGATCGCCCCCCACCGCCTTACAGCAAGGGCTCCGACTGGTACTCCTATG
  CCCTCCTTGGTCCACAAGGAACTGCACCAAGGGAGCCACTTTTGGGTAATTGTGCCTTAG
  CCATCCCGGCGAAACAAATCGCCCTCCATCCGGAAGCCGCAGTGTCCCACTGAACCCAGT
  ACTTGAAAGGCCAACTCGTCGACCATTGCAATGTGGTCCTGCAACCTGGCTGGAAACACC
  CATCCCTGGTTCCGGTCCTGAATCAATTGCGCTCGCGAATACTGTTTACATTTGTGGCCA
  ACCCACACANACCCATTTGTCTTTCCGGAACTCTCGCTTCTCGATGAAAATGTCCCCGGA
  TTGCTCTTTAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013402
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013402.3, NP_037534.2
• RefSeq Size: 4432 bp
• RefSeq ORF: 1335 bp
• Locus ID: 3992
• UniProt ID: O60427
• Cytogenetics: 11q12.2
• Domains: heme_1, FA_desaturase
• Protein Families: Transcription Factors, Transmembrane
• Protein Pathways: Biosynthesis of unsaturated fatty acids
• Gene Summary: The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008]