VARS2 (NM_020442) Human Untagged Clone

CAT#: SC318474

VARS2 (untagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VARS2
• Synonyms: COXPD20; VALRS; VARS2L; VARSL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318474 representing NM_020442.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCTCATTTGCCTCTCGCCTCTTTTCGACCACCATTTTGGGGGCTGAGGCACTCACGGGGCCTCCCC
  AGGTTTCACTCCGTTTCTACACAGTCGGAGCCCCATGGATCTCCCATCTCCCGGAGGAACCGTGAAGCC
  AAACAGAAGCGCCTGCGAGAGAAGCAGGCGACTCTGGAGGCTGAGATAGCAGGGGAGAGCAAGTCACCT
  GCAGAATCCATTAAGGCCTGGAGGCCTAAGGAGTTAGTATTGTATGAAATCCCTACGAAACCCGGTGAA
  AAGAAAGATGTCTCTGGGCCCCTGCCTCCTGCATACAGCCCCCGATATGTTGAGGCTGCCTGGTACCCG
  TGGTGGGTACGAGAGGGCTTCTTCAAACCAGAATATCAGGCCCGGCTGCCCCAAGCTACAGGGGAGACC
  TTTTCCATGTGTATCCCACCTCCCAATGTCACTGGCTCCCTGCACATTGGCCACGCACTCACGGTGGCC
  ATACAGGATGCCCTCGTGCGCTGGCACCGGATGCGTGGGGATCAAGTGCTGTGGGTCCCTGGTTCAGAT
  CATGCAGGAATTGCTACACAAGCTGTGGTGGAGAAACAACTGTGGAAGGAACGGGGAGTGAGGAGACAT
  GAGCTGAGCCGGGAGGCCTTCCTTAGGGAGGTGTGGCAGTGGAAGGAGGCGAAAGGTGGAGAGATCTGT
  GAGCAGCTGCGAGCTCTGGGTGCCTCCCTGGACTGGGATCGAGAGTGTTTTACCATGGATGTTGGCTCC
  TCAGTGGCTGTGACTGAAGCTTTTGTGCGGCTCTACAAGGCGGGGTTGCTGTACCGGAACCATCAGCTT
  GTCAACTGGTCATGTGCTTTAAGATCAGCCATCTCGGACATTGAGGTGGAGAACCGGCCCCTGCCTGGC
  CACACACAGCTTCGACTGCCTGGCTGCCCCACCCCCGTGTCTTTTGGCCTCCTATTTTCTGTTGCCTTC
  CCCGTGGATGGAGAGCCTGATGCAGAGGTTGTGGTAGGAACCACAAGGCCAGAGACGCTGCCTGGAGAT
  GTGGCTGTGGCCGTTCATCCAGACGACTCGCGATACACACATCTACACGGGCGACAGCTTCGTCACCCC
  TTGATGGGGCAGCCTCTTCCCCTCATCACAGACTATGCTGTTCAGCCACATGTGGGCACGGGGGCAGTG
  AAGGTGACTCCAGCTCACAGTCCTGCCGATGCTGAGATGGGGGCCCGACATGGCTTGAGCCCCTTGAAT
  GTCATTGCGGAGGATGGGACCATGACCTCTCTCTGCGGGGACTGGCTGCAGGGTCTTCACCGGTTTGTG
  GCCCGGGAAAAGATAATGTCTGTGCTGAGTGAACGGGGCCTGTTCCGGGGCCTCCAGAACCACCCCATG
  GTACTGCCCATCTGCAGCCGTTCTGGGGATGTGATAGAATACCTGCTGAAGAACCAGTGGTTTGTCCGC
  TGCCAGGAAATGGGGGCCCGAGCTGCCAAGGCTGTGGAGTCGGGGGCCCTGGAGCTCAGTCCCTCCTTC
  CACCAGAAGAACTGGCAGCACTGGTTTTCCCATATTGGGGACTGGTGTGTCTCCCGGCAGCTGTGGTGG
  GGCCATCAGATTCCAGCCTACCTGGTTGTAGAGGACCATGCGCAGGGAGAAGAGGACTGTTGGGTGGTC
  GGGCGGTCAGAGGCTGAGGCCAGAGAGGTAGCAGCGGAACTGACAGGGAGGCCAGGGGCAGAGCTGACC
  CTGGAGAGGGATCCTGATGTCCTAGACACATGGTTTTCTTCTGCCCTGTTCCCCTTTTCTGCCCTGGGC
  TGGCCCCAAGAGACCCCAGACCTTGCTCGTTTCTACCCCCTGTCACTTTTGGAAACGGGCAGCGACCTT
  CTGCTGTTCTGGGTGGGCCGCATGGTCATGTTGGGGACCCAGCTCACAGGGCAGCTGCCCTTCAGCAAG
  GTGCTTCTTCATCCCATGGTTCGGGACAGGCAGGGCCGGAAGATGAGCAAGTCCCTGGGGAATGTGCTG
  GACCCAAGAGACATCATCAGTGGGGTGGAGATGCAGGTGCTGCAGGAAAAGCTGAGAAGCGGAAATTTG
  GACCCTGCAGAGCTGGCCATTGTGGCTGCAGCACAGAAAAAGGACTTTCCTCACGGGATCCCTGAGTGT
  GGGACAGATGCCCTGAGATTCACACTCTGCTCCCATGGAGTTCAGGCGGGCGACTTGCACCTGTCAGTC
  TCTGAGGTCCAGAGCTGCCGACATTTCTGCAACAAGATCTGGAATGCTCTTCGCTTTATCCTCAATGCT
  TTAGGGGAGAAATTTGTGCCACAGCCTGCTGAGGAGCTGTCTCCCTCCTCCCCGATGGATGCCTGGATC
  CTGAGCCGCCTTGCCCTGGCTGCCCAGGAGTGTGAGCGGGGCTTCCTCACCCGAGAGCTCTCGCTCGTC
  ACTCATGCCCTGCACCACTTCTGGCTTCACAACCTCTGTGACGTCTACCTGGAGGCTGTGAAGCCCGTG
  CTGTGGCACTCGCCCCGCCCCCTGGGGCCCCCTCAGGTCCTGTTCTCCTGCGCTGACCTCGGCCTCCGC
  CTCCTGGCCCCACTGATGCCCTTCCTGGCTGAAGAGCTCTGGCAGAGGCTGCCCCCCAGGCCTGGTTGC
  CCCCCTGCCCCCAGCATCTCGGTTGCCCCCTACCCCAGCGCCTGCAGCTTGGAGCACTGGCGCCAGCCA
  GAGCTGGAGCGGCGCTTCTCCCGGGTCCAAGAGGTCGTGCAGGTGCTAAGGGCTCTCCAAGCCACGTAC
  CAGCTCACCAAAGCCCGGCCCCGAGTGCTGCTGCAGAGCTCAGAGCCTGGGGACCAGGGCCTCTTCGAG
  GCCTTCTTGGAGCCCCTGGGCACCCTGGGCTACTGTGGGGCTGTGGGCCTGTTACCCCCAGGCGCAGCA
  GCTCCCTCCGGCTGGGCCCAGGCTCCACTCAGTGACACGGCTCAAGTCTACATGGAGCTGCAGGGCCTG
  GTGGACCCGCAGATCCAGCTACCTCTGTTAGCCGCCCGAAGGTACAAGTTGCAGAAGCAGCTTGATAGC
  CTCACAGCCAGGACCCCATCAGAAGGGGAGGCAGGGACTCAGAGGCAACAAAAGCTTTCTTCCCTCCAG
  CTGGAATTGTCAAAACTGGACAAGGCAGCCTCTCACCTCCGGCAGCTGATGGATGAGCCTCCAGCCCCA
  GGGAGCCCGGAGCTCTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_020442
• Insert Size: 3192 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020442.3
• RefSeq Size: 3622 bp
• RefSeq ORF: 3192 bp
• Locus ID: 57176
• UniProt ID: Q5ST30
• Cytogenetics: 6p21.33
• Domains: tRNA-synt_1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
• MW: 118.4 kDa
• Gene Summary: This gene encodes a mitochondrial aminoacyl-tRNA synthetase, which catalyzes the attachment of valine to tRNA(Val) for mitochondrial translation. Mutations in this gene cause combined oxidative phosphorylation deficiency-20, and are also associated with early-onset mitochondrial encephalopathies. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2014]
  Transcript Variant: This variant (2) contains alternate 5' exon structure, and it thus differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) is shorter at the N-terminus, compared to isoform 1.