OVCA1 (DPH1) (NM_001383) Human Untagged Clone

CAT#: SC317727

DPH1 (untagged)-Human DPH1 homolog (S. cerevisiae) (DPH1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OVCA1
• Synonyms: DEDSSH; DPH2L; DPH2L1; OVCA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317727 representing NM_001383.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCGCAGGCAGGTGATGGCGGCGCTGGTCGTATCCGGGGCAGCGGAGCAGGGCGGCCGAGACGGCCCT
  GGCAGAGGTCGGGCCCCTCGGGGCCGCGTGGCCAATCAGATCCCCCCTGAGATCCTGAAGAACCCTCAG
  CTGCAGGCAGCAATCCGGGTCCTGCCTTCCAACTACAACTTTGAGATCCCCAAGACCATCTGGAGGATC
  CAACAAGCCCAGGCCAAGAAGGTGGCCTTGCAAATGCCGGAAGGCCTCCTCCTCTTTGCCTGTACCATT
  GTGGATATCTTGGAAAGGTTCACGGAGGCCGAAGTGATGGTGATGGGTGACGTGACCTACGGGGCTTGC
  TGTGTGGATGACTTCACAGCGAGGGCCCTGGGAGCTGACTTCTTGGTGCACTACGGCCACAGTTGCCTG
  ATTCCCATGGACACCTCGGCCCAAGACTTCCGGGTGCTGTACGTCTTTGTGGACATCCGGATAGACACT
  ACACACCTCCTGGACTCTCTCCGCCTCACCTTTCCCCCAGCCACTGCCCTTGCCCTGGTCAGCACCATT
  CAGTTTGTGTCGACCTTGCAGGCAGCCGCCCAGGAGCTGAAAGCCGAGTATCGTGTGAGTGTCCCACAG
  TGCAAGCCCCTGTCCCCTGGAGAGATCCTGGGCTGCACATCCCCCCGACTGTCCAAAGAGGTGGAGGCC
  GTTGTGTATCTTGGAGATGGCCGCTTCCATCTGGAGTCTGTCATGATTGCCAACCCCAATGTCCCCGCT
  TACCGGTATGACCCATATAGCAAAGTCCTATCCAGAGAACACTATGACCACCAGCGCATGCAGGCTGCT
  CGCCAAGAAGCCATAGCCACTGCCCGCTCAGCTAAGTCCTGGGGCCTTATTCTGGGCACTTTGGGCCGC
  CAGGGCAGTCCTAAGATCCTGGAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGG
  CTGCTGCTCTCTGAGATCTTCCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTG
  GCATGTCCACGTCTCTCCATTGACTGGGGCACAGCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGCG
  GCCGTGGCTCTGAGGGACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTG
  GGGCCCTGGACGGTGAACCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAG
  GTGCAGGAGGGGTCCGCGCGTCCCCCTTCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGACGAGAAG
  GTGGCGCCGCTGGCTCCTTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001383
• Insert Size: 1332 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001383.3
• RefSeq Size: 2200 bp
• RefSeq ORF: 1332 bp
• Locus ID: 1801
• UniProt ID: Q9BZG8
• Cytogenetics: 17p13.3
• Domains: Diphthamide_syn
• MW: 48.8 kDa
• Gene Summary: The protein encoded by this gene is an enzyme involved in the biosynthesis of diphthamide, a modified histidine found only in elongation factor-2 (EEF2). Diphthamide residues in EEF2 are targeted for ADP-ribosylation by diphtheria toxin and Pseudomonas exotoxin A. Defects in this gene have been associated with both ovarian cancer and autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies. [provided by RefSeq, Oct 2016]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.