NALP4 (NLRP4) (NM_134444) Human Untagged Clone

CAT#: SC305988

NLRP4 (untagged)-Human NLR family, pyrin domain containing 4 (NLRP4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NALP4
• Synonyms: CLR19.5; CT58; NALP4; PAN2; PYPAF4; RNH2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_134444 edited
  GGAGGAGCTGGAGAACATAGACAGGGATGAGGTTTTATTTATTTATTGTTCCTGGTCACT
  GTCTCTTTGAGGATTGGTATCTCTGCTCCAGAAAAGATGGCAGCCTCTTTCTTCTCTGAT
  TTTGGTCTTATGTGGTATCTGGAGGAGCTCAAAAAGGAGGAGTTCAGGAAATTTAAAGAA
  CATCTCAAGCAAATGACTTTGCAGCTTGAACTCAAGCAGATTCCCTGGACTGAGGTCAAA
  AAAGCATCCCGGGAAGAACTTGCAAACCTCTTGATCAAGCACTATGAAGAACAACAAGCT
  TGGAACATAACCTTAAGAATCTTTCAAAAGATGGATAGAAAGGATCTCTGCATGAAGGTC
  ATGAGGGAGAGAACAGGATACACAAAGACCTATCAAGCTCACGCAAAGCAGAAATTCAGC
  CGCTTATGGTCCAGCAAGTCTGTCACTGAGATTCACCTATACTTTGAGGAGGAAGTCAAG
  CAAGAAGAATGTGACCATTTGGACCGCCTTTTTGCTCCCAAGGAAGCTGGGAAACAGCCA
  CGTACAGTGATCATTCAAGGACCACAAGGAATTGGAAAAACGACACTCCTGATGAAGCTG
  ATGATGGCCTGGTCGGACAACAAGATCTTTCGGGATAGGTTCCTGTACACATTCTATTTC
  TGCTGCAGAGAACTGAGGGAGTTGCCGCCAACGAGTTTGGCTGACTTGATTTCCAGAGAG
  TGGCCTGACCCCGCTGCTCCTATAACAGAGATCGTGTCTCAACCGGAGAGACTCTTGTTC
  GTCATCGACAGCTTCGAAGAGCTGCAGGGCGGCTTGAACGAACCCGATTCGGATCTGTGT
  GGTGACTTGATGGAGAAACGGCCGGTGCAGGTGCTTCTGAGCAGTTTGCTGAGGAAGAAG
  ATGCTCCCGGAGGCCTCCCTGCTCATCGCTATCAAACCCGTGTGCCCGAAGGAGCTCCGG
  GATCAGGTGACGATCTCAGAAATCTACCAGCCCCGGGGATTCAACGAGAGTGATAGGTTA
  GTGTATTTCTGCTGTTTCTTCAAAGACCCGAAAAGAGCCATGGAAGCCTTCAATCTTGTA
  AGAGAAAGTGAACAGCTGTTTTCCATATGCCAAATCCCGCTCCTCTGCTGGATCCTGTGT
  ACCAGTCTGAAGCAAGAGATGCAGAAAGGAAAAGACCTGGCCCTGACCTGCCAGAGCACT
  ACCTCTGTGTACTCCTCTTTCGTCTTTAACCTGTTCACACCTGACGGTGCCGAGGGCCCG
  ACTCAGCAAACCCAGCACCAGCTGAAGGCCCTGTGCTCCCTGGCTGCAGAGGGTATGTGG
  ACAGACACATTTGAGTTTTGTGAAGACGACCTCCGGAGAAATGGGGTTGTTGACGCTGAC
  ATCCCTGCGCTGCTGGGCACCAAGATACTTCTGAAGTACGGGGAGCGTGAGAGCTCCTAC
  GTGTTCCTCCACGTGTGTATCCAGGAGTTCTGTGCCGCCTTGTTCTATTTGCTCAAGAGC
  CACCTTGATCATCCTCACCCAGCTGTGAGATGTGTACAGGAATTGCTAGTTGCCAATTTT
  GAAAAAGCAAGGAGAGCACATTGGATTTTTTTGGGGTGTTTTCTAACTGGCCTTTTAAAT
  AAAAAGGAACAAGAAAAACTGGATGCGTTTTTTGGCTTCCAACTGTCCCAAGAGATAAAG
  CAGCAAATTCACCAGTGCCTGAAGAGCTTAGGGGAGCGTGGCAATCCTCAGGGACAGGTG
  GATTCCTTGGCGATATTTTACTGTCTCTTTGAAATGCAGGATCCTGCCTTTGTGAAGCAG
  GCAGTGAACCTCCTCCAAGAAGCTAACTTTCATATTATTGACAACGTGGACTTGGTGGTT
  TCTGCCTACTGCTTAAAATACTGCTCCAGCTTGAGGAAACTCTGTTTTTCCGTTCAAAAT
  GTCTTTAAGAAAGAGGATGAACACAGCTCTACGTCGGATTACAGCCTCATCTGTTGGCAT
  CACATCTGCTCTGTGCTCACCACCAGCGGGCACCTCAGAGAGCTCCAGGTGCAGGACAGC
  ACCCTCAGCGAGTCGACCTTTGTGACCTGGTGTAACCAGCTGAGGCATCCCAGCTGTCGC
  CTTCAGAAGCTTGGAATAAATAACGTTTCCTTTTCTGGCCAGAGTGTTCTGCTCTTTGAG
  GTGCTCTTTTATCAGCCAGACTTGAAATACCTGAGCTTCACCCTCACGAAACTCTCTCGT
  GATGACATCAGGTCCCTCTGTGATGCCTTGAACTACCCAGCAGGCAACGTCAAAGAGCTA
  GCGCTGGTAAATTGTCACCTCTCACCCATTGATTGTGAAGTCCTTGCTGGCCTTCTAACC
  AACAACAAGAAGCTGACGTATCTGAATGTATCCTGCAACCAGTTAGACACAGGCGTGCCC
  CTTTTGTGTGAAGCCATGTGCAGCCCAGACACGGTCCTGGTATACCTGATGTTGGCTTTC
  TGCCACCTCAGCGAGCAGTGCTGCGAATACATCTCTGAAATGCTTCTGCGTAACAAGAGC
  GTGCGCTATCTAGACCTCAGTGCCAATGTCCTGAAGGACGAAGGACTGAAAACTCTCTGC
  GAGGCCTTGAAACATCCGGACTGCTGCCTGGATTCACTGTGTTTGGTAAAATGTTTTATC
  ACTGCTGCTGGCTGTGAAGACCTCGCCTCTGCTCTCATCAGCAATCAAAACCTGAAGATT
  CTGCAAATTGGGTGCAATGAAATCGGAGATGTGGGTGTGCAGCTGTTGTGTCGGGCTCTG
  ACGCATACGGATTGCCGCTTAGAGATTCTTGGGTTGGAAGAATGTGGGTTAACGAGCACC
  TGCTGTAAGGATCTCGCGTCTGTTCTCACCTGCAGTAAGACCCTGCAGCTGCTCAACCTG
  ACCTTGAACACCTTGGACCACACAGGGGTGGTTGTACTCTGTGAGGCCCTGAGACACCCA
  GAGTGTGCCCTGCAGGTGCTCGGGCTGAGAAAAACTGATTTTGATGAGGAAACCCAGGCA
  CTGCTGACGGCTGAGGAAGAGAGAAATCCTAACCTGACCATCACAGACGACTGTGACACA
  ATCACAAGGGTAGAGATCTGATTGCGAGGAACCTGGGCTCTGACTCGAACACCTGCAAAG
  GACAGGGACTGGGACCGTTACTTACATGACACTGCACCCAGGAGATACAAATCATTGACA
  CTCTGAGTTGTGAGATTTCTGGCACCCCATTCATAGATTTGATATGATACACGTGGTTTT
  TATGTGCTCTGTGGCCTTGGATGAGTCACTGAAAGGCCTTCATGGTCTCTCGGTCTCACA
  AGGACCTCTTAACCCCTCAATAAAGTGTTACATTTCTAAACATTAAAAGAAAAAAAAAAA
  AAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_134444
• Insert Size: 3400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain 4 SNPs compared with reference sequence NM_134444.3.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134444.3, NP_604393.1
• RefSeq Size: 3339 bp
• RefSeq ORF: 2985 bp
• Locus ID: 147945
• UniProt ID: Q96MN2
• Cytogenetics: 19q13.43
• Gene Summary: The protein encoded by this gene is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family, and is predicted to contain an N-terminal pyrin effector domain (PYD), a centrally-located nucleotide-binding and oligomerization domain (NACHT) and C-terminal leucine-rich repeats (LRR). This gene product has a demonstrated role as a negative regulator of autophagy and type I interferon signaling pathways as a result of protein interactions with its NACHT domain. The PYD domain has also been shown to be important in the inhibition of NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells). [provided by RefSeq, Dec 2016]