NALP4 (NLRP4) (NM_134444) Human Tagged ORF Clone

CAT#: RC207991

NLRP4 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 4 (NLRP4)

Product Images

Circular map for RC207991

• 

  Western blot validation of overexpression lysate (Cat# LY408727) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207991 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NLRP4 protein (Cat# TP307991). The protein was produced from HEK293T cells transfected with NLRP4 cDNA clone (Cat# RC207991) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NALP4
• Synonyms: CLR19.5; CT58; NALP4; PAN2; PYPAF4; RNH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207991 representing NM_134444
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCTCTTTCTTCTCTGATTTTGGTCTTATGTGGTATCTGGAGGAGCTCAAAAAGGAGGAGTTCA
  GGAAATTTAAAGAACATCTCAAGCAAATGACTTTGCAGCTTGAACTCAAGCAGATTCCCTGGACTGAGGT
  CAAAAAAGCATCCCGGGAAGAACTTGCAAACCTCTTGATCAAGCACTATGAAGAACAACAAGCTTGGAAC
  ATAACCTTAAGAATCTTTCAAAAGATGGATAGAAAGGATCTCTGCATGAAGGTCATGAGGGAGAGAACAG
  GATACACAAAGACCTATCAAGCTCACGCAAAGCAGAAATTCAGCCGCTTATGGTCCAGCAAGTCTGTCAC
  TGAGATTCACCTATACTTTGAGGAGGAAGTCAAGCAAGAAGAATGTGACCATTTGGACCGCCTTTTTGCT
  CCCAAGGAAGCTGGGAAACAGCCACGTACAGTGATCATTCAAGGACCACAAGGAATTGGAAAAACGACAC
  TCCTGATGAAGCTGATGATGGCCTGGTCGGACAACAAGATCTTTCGGGATAGGTTCCTGTACACATTCTA
  TTTCTGCTGCAGAGAACTGAGGGAGTTGCCGCCAACGAGTTTGGCTGACTTGATTTCCAGAGAGTGGCCT
  GACCCCGCTGCTCCTATAACAGAGATCGTGTCTCAACCGGAGAGACTCTTGTTCGTCATCGACAGCTTCG
  AAGAGCTGCAGGGCGGCTTGAACGAACCCGATTCGGATCTGTGTGGTGACTTGATGGAGAAACGGCCGGT
  GCAGGTGCTTCTGAGCAGTTTGCTGAGGAAGAAGATGCTCCCGGAGGCCTCCCTGCTCATCGCTATCAAA
  CCCGTGTGCCCGAAGGAGCTCCGGGATCAGGTGACGATCTCAGAAATCTACCAGCCCCGGGGATTCAACG
  AGAGTGATAGGTTAGTGTATTTCTGCTGTTTCTTCAAAGACCCGAAAAGAGCCATGGAAGCCTTCAATCT
  TGTAAGAGAAAGTGAACAGCTGTTTTCCATATGCCAAATCCCGCTCCTCTGCTGGATCCTGTGTACCAGT
  CTGAAGCAAGAGATGCAGAAAGGAAAAGACCTGGCCCTGACCTGCCAGAGCACTACCTCTGTGTACTCCT
  CTTTCGTCTTTAACCTGTTCACACCTGACGGTGCCGAGGGCCCGACTCAGCAAACCCAGCACCAGCTGAA
  GGCCCTGTGCTCCCTGGCTGCAGAGGGTATGTGGACAGACACATTTGAGTTTTGTGAAGACGACCTCCGG
  AGAAATGGGGTTGTTGACGCTGACATCCCTGCGCTGCTGGGCACCAAGATACTTCTGAAGTACGGGGAGC
  GTGAGAGCTCCTACGTGTTCCTCCACGTGTGTATCCAGGAGTTCTGTGCCGCCTTGTTCTATTTGCTCAA
  GAGCCACCTTGATCATCCTCACCCAGCTGTGAGATGTGTACAGGAATTGCTAGTTGCCAATTTTGAAAAA
  GCAAGGAGAGCACATTGGATTTTTTTGGGGTGTTTTCTAACTGGCCTTTTAAATAAAAAGGAACAAGAAA
  AACTGGATGCGTTTTTTGGCTTCCAACTGTCCCAAGAGATAAAGCAGCAAATTCACCAGTGCCTGAAGAG
  CTTAGGGGAGCGTGGCAATCCTCAGGGACAGGTGGATTCCTTGGCGATATTTTACTGTCTCTTTGAAATG
  CAGGATCCTGCCTTTGTGAAGCAGGCAGTGAACCTCCTCCAAGAAGCTAACTTTCATATTATTGACAACG
  TGGACTTGGTGGTTTCTGCCTACTGCTTAAAATACTGCTCCAGCTTGAGGAAACTCTGTTTTTCCGTTCA
  AAATGTCTTTAAGAAAGAGGATGAACACAGCTCTACGTCGGATTACAGCCTCATCTGTTGGCATCACATC
  TGCTCTGTGCTCACCACCAGCGGGCACCTCAGAGAGCTCCAGGTGCAGGACAGCACCCTCAGCGAGTCGA
  CCTTTGTGACCTGGTGTAACCAGCTGAGGCATCCCAGCTGTCGCCTTCAGAAGCTTGGAATAAATAACGT
  TTCCTTTTCTGGCCAGAGTGTTCTGCTCTTTGAGGTGCTCTTTTATCAGCCAGACTTGAAATACCTGAGC
  TTCACCCTCACGAAACTCTCTCGTGATGACATCAGGTCCCTCTGTGATGCCTTGAACTACCCAGCAGGCA
  ACGTCAAAGAGCTAGCGCTGGTAAATTGTCACCTCTCACCCATTGATTGTGAAGTCCTTGCTGGCCTTCT
  AACCAACAACAAGAAGCTGACGTATCTGAATGTATCCTGCAACCAGTTAGACACAGGCGTGCCCCTTTTG
  TGTGAAGCCATGTGCAGCCCAGACACGGTCCTGGTATACCTGATGTTGGCTTTCTGCCACCTCAGCGAGC
  AGTGCTGCGAATACATCTCTGAAATGCTTCTGCGTAACAAGAGCGTGCGCTATCTAGACCTCAGTGCCAA
  TGTCCTGAAGGACGAAGGACTGAAAACTCTCTGCGAGGCCTTGAAACATCCGGACTGCTGCCTGGATTCA
  CTGTGTTTGGTAAAATGTTTTATCACTGCTGCTGGCTGTGAAGACCTCGCCTCTGCTCTCATCAGCAATC
  AAAACCTGAAGATTCTGCAAATTGGGTGCAATGAAATCGGAGATGTGGGTGTGCAGCTGTTGTGTCGGGC
  TCTGACGCATACGGATTGCCGCTTAGAGATTCTTGGGTTGGAAGAATGTGGGTTAACGAGCACCTGCTGT
  AAGGATCTCGCGTCTGTTCTCACCTGCAGTAAGACCCTGCAGCTGCTCAACCTGACCTTGAACACCTTGG
  ACCACACAGGGGTGGTTGTACTCTGTGAGGCCCTGAGACACCCAGAGTGTGCCCTGCAGGTGCTCGGGCT
  GAGAAAAACTGATTTTGATGAGGAAACCCAGGCACTGCTGACGGCTGAGGAAGAGAGAAATCCTAACCTG
  ACCATCACAGACGACTGTGACACAATCACAAGGGTAGAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207991 representing NM_134444
  Red=Cloning site Green=Tags(s)
  
  MAASFFSDFGLMWYLEELKKEEFRKFKEHLKQMTLQLELKQIPWTEVKKASREELANLLIKHYEEQQAWN
  ITLRIFQKMDRKDLCMKVMRERTGYTKTYQAHAKQKFSRLWSSKSVTEIHLYFEEEVKQEECDHLDRLFA
  PKEAGKQPRTVIIQGPQGIGKTTLLMKLMMAWSDNKIFRDRFLYTFYFCCRELRELPPTSLADLISREWP
  DPAAPITEIVSQPERLLFVIDSFEELQGGLNEPDSDLCGDLMEKRPVQVLLSSLLRKKMLPEASLLIAIK
  PVCPKELRDQVTISEIYQPRGFNESDRLVYFCCFFKDPKRAMEAFNLVRESEQLFSICQIPLLCWILCTS
  LKQEMQKGKDLALTCQSTTSVYSSFVFNLFTPDGAEGPTQQTQHQLKALCSLAAEGMWTDTFEFCEDDLR
  RNGVVDADIPALLGTKILLKYGERESSYVFLHVCIQEFCAALFYLLKSHLDHPHPAVRCVQELLVANFEK
  ARRAHWIFLGCFLTGLLNKKEQEKLDAFFGFQLSQEIKQQIHQCLKSLGERGNPQGQVDSLAIFYCLFEM
  QDPAFVKQAVNLLQEANFHIIDNVDLVVSAYCLKYCSSLRKLCFSVQNVFKKEDEHSSTSDYSLICWHHI
  CSVLTTSGHLRELQVQDSTLSESTFVTWCNQLRHPSCRLQKLGINNVSFSGQSVLLFEVLFYQPDLKYLS
  FTLTKLSRDDIRSLCDALNYPAGNVKELALVNCHLSPIDCEVLAGLLTNNKKLTYLNVSCNQLDTGVPLL
  CEAMCSPDTVLVYLMLAFCHLSEQCCEYISEMLLRNKSVRYLDLSANVLKDEGLKTLCEALKHPDCCLDS
  LCLVKCFITAAGCEDLASALISNQNLKILQIGCNEIGDVGVQLLCRALTHTDCRLEILGLEECGLTSTCC
  KDLASVLTCSKTLQLLNLTLNTLDHTGVVVLCEALRHPECALQVLGLRKTDFDEETQALLTAEEERNPNL
  TITDDCDTITRVEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134444
• ORF Size: 2982 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134444.5
• RefSeq Size: 3339 bp
• RefSeq ORF: 2985 bp
• Locus ID: 147945
• UniProt ID: Q96MN2
• Cytogenetics: 19q13.43
• MW: 113.2 kDa
• Gene Summary: The protein encoded by this gene is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family, and is predicted to contain an N-terminal pyrin effector domain (PYD), a centrally-located nucleotide-binding and oligomerization domain (NACHT) and C-terminal leucine-rich repeats (LRR). This gene product has a demonstrated role as a negative regulator of autophagy and type I interferon signaling pathways as a result of protein interactions with its NACHT domain. The PYD domain has also been shown to be important in the inhibition of NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells). [provided by RefSeq, Dec 2016]