NrCAM (NM_001037132) Human Untagged Clone

CAT#: SC302851

NRCAM (untagged)-Human neuronal cell adhesion molecule (NRCAM), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NRCAM
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001037132 edited
  ATGCAGCTTAAAATAATGCCGAAAAAGAAGCGCTTATCTGCGGGCAGAGTGCCCCTGATT
  CTCTTCCTGTGCCAGATGATTAGTGCACTGGAAGTACCTCTTGATCCAAAACTTCTTGAA
  GACTTGGTACAGCCTCCAACCATCACCCAACAGTCTCCAAAAGATTACATTATTGACCCT
  CGGGAGAATATTGTAATCCAGTGTGAAGCCAAAGGGAAACCGCCCCCAAGCTTTTCCTGG
  ACCCGTAATGGGACTCATTTTGACATCGATAAAGACCCTCTGGTCACCATGAAGCCTGGC
  ACAGGAACGCTCATAATTAACATCATGAGCGAAGGGAAAGCTGAGACCTATGAAGGAGTC
  TATCAGTGTACAGCAAGGAACGAACGCGGAGCTGCAGTTTCTAATAACATTGTTGTCCGC
  CCATCCAGATCACCATTGTGGACCAAAGAAAAACTTGAACCAATCACACTTCAAAGTGGT
  CAGTCTTTAGTACTTCCCTGCAGACCCCCAATTGGATTACCACCACCTATAATATTTTGG
  ATGGATAATTCCTTTCAAAGACTTCCACAAAGTGAGAGAGTTTCTCAAGGTTTGAATGGG
  GACCTTTATTTTTCCAATGTCCTCCCAGAGGACACCCGCGAAGACTATATCTGTTATGCT
  AGATTTAATCATACTCAAACCATACAGCAGAAGCAACCTATTTCTGTGAAGGTGATTTCA
  GTGGATGAATTGAATGACACTATAGCTGCTAATTTGAGTGACACTGAGTTTTATGGTGCT
  AAATCAAGTAGAGAGAGGCCACCAACATTTTTAACTCCAGAAGGCAATGCAAGTAACAAA
  GAGGAATTAAGAGGAAATGTGCTTTCACTGGAGTGCATTGCAGAAGGACTGCCTACCCCA
  ATTATTTACTGGGCAAAGGAAGATGGAATGCTACCCAAAAACAGGACAGTTTATAAGAAC
  TTTGAGAAAACCTTGCAGATCATTCATGTTTCAGAAGCAGACTCTGGAAATTACCAATGT
  ATAGCAAAAAATGCATTAGGAGCCATCCACCATACCATTTCTGTTAGAGTTAAAGCGGCT
  CCATACTGGATCACAGCCCCTCAAAATCTTGTGCTGTCCCCAGGAGAGGATGGGACCTTG
  ATCTGCAGAGCTAATGGCAACCCCAAACCCAGAATTAGCTGGTTAACAAATGGAGTCCCA
  ATAGAAATTGCCCCTGATGACCCCAGCAGAAAAATAGATGGCGATACCATTATTTTTTCA
  AATGTTCAAGAAAGATCAAGTGCAGTATATCAGTGCAATGCCTCTAATGAATATGGATAT
  TTACTGGCAAACGCATTTGTAAATGTGCTGGCTGAGCCACCACGAATCCTCACACCTGCA
  AACACACTCTACCAGGTCATTGCAAACAGGCCTGCTTTACTAGACTGTGCCTTCTTTGGG
  TCTCCTCTCCCAACCATCGAGTGGTTTAAAGGAGCTAAAGGAAGTGCTCTTCATGAAGAT
  ATTTATGTTTTACATGAAAATGGAACTTTGGAAATTCCTGTGGCCCAAAAGGACAGTACA
  GGAACTTATACGTGTGTTGCAAGGAATAAATTAGGGATGGCAAAGAATGAAGTTCACTTA
  GAAATCAAAGATGCTACATGGATCGTTAAACAGCCCGAATATGCAGTTGTGCAAAGAGGG
  AGCATGGTGTCCTTTGAATGCAAAGTGAAACATGATCACACCTTATCCCTCACTGTCCTG
  TGGCTGAAGGACAACAGGGAACTGCCCAGTGATGAAAGGTTCACTGTTGACAAGGATCAT
  CTAGTGGTAGCTGATGTCAGTGACGATGACAGCGGGACCTACACGTGTGTGGCCAACACC
  ACTCTGGACAGCGTCTCCGCCAGCGCTGTGCTTAGCGTTGTTGCTCCTACTCCAACTCCA
  GCTCCCGTTTACGATGTCCCAAATCCTCCCTTTGACTTAGAACTGACAGATCAACTTGAC
  AAAAGTGTTCAGCTGTCATGGACCCCAGGCGATGACAACAATAGCCCCATTACAAAATTC
  ATCATCGAATATGAAGATGCAATGCACAAGCCAGGGCTGTGGCACCACCAAACTGAAGTT
  TCTGGAACACAGACCACAGCCCAGCTGAAGCTGTCTCCTTACGTGAACTACTCCTTCCGC
  GTGATGGCAGTGAACAGCATTGGGAAGAGCTTGCCCAGCGAGGCCTCTGAGCAGTATTTG
  ACGAAAGCCTCAGAACCAGATAAAAACCCCACAGCTGTGGAAGGACTGGGATCAGAGCCT
  GATAATTTGGTGATTACGTGGAAGCCCTTGAATGGTTTCGAATCTAATGGGCCAGGCCTT
  CAGTACAAAGTTAGCTGGCGCCAGAAAGATGGTGATGATGAATGGACATCTGTGGTTGTG
  GCAAATGTATCCAAATATATTGTCTCAGGCACGCCAACCTTTGTTCCATACCTGATCAAA
  GTTCAGGCCCTGAATGACATGGGGTTTGCCCCCGAGCCAGCTGTAGTCATGGGACATTCT
  GGAGAAGACCTCCCAATGGTGGCTCCTGGGAACGTGCGTGTGAATGTGGTGAACAGTACC
  TTAGCCGAGGTGCACTGGGACCCAGTACCTCTGAAAAGCATCCGAGGACACCTACAAGGC
  TATCGGATTTACTATTGGAAGACCCAGAGTTCATCTAAAAGAAACAGACGTCACATTGAG
  AAAAAGATCCTCACCTTCCAAGGCAGCAAGACTCATGGCATGTTGCCGGGGCTAGAGCCC
  TTTAGCCACTACACACTGAATGTCCGAGTGGTCAATGGGAAAGGGGAGGGCCCAGCCAGC
  CCTGACAGAGTCTTTAATACTCCAGAAGGAGTCCCCAGTGCTCCCTCGTCTTTGAAGATT
  GTGAATCCAACACTGGACTCTCTCACTTTGGAATGGGATCCACCGAGCCACCCGAATGGC
  ATTTTGACAGAGTACACCTTAAAGTATCAGCCAATTAACAGCACACATGAATTAGGCCCT
  CTGGTAGATTTGAAAATTCCTGCCAACAAGACACGGTGGACTTTAAAAAATTTAAATTTC
  AGCACTCGATATAAGTTTTATTTCTATGCACAAACATCAGCAGGATCAGGAAGTCAAATT
  ACAGAGGAAGCAGTAACAACTGTGGATGAAGCTGGTATTCTTCCACCTGATGTAGGTGCA
  GGCAAAGTTCAAGCAGTAAATCCCAGGATCAGCAATCTTACTGCTGCAGCTGCTGAGACC
  TATGCCAATATCAGTTGGGAATATGAGGGACCAGAGCATGTGAACTTTTATGTTGAATAT
  GGTGTAGCAGGCAGCAAAGAAGAATGGAGAAAAGAAATTGTAAATGGTTCTCGGAGCTTC
  TTTGGGTTAAAGGGTCTAATGCCAGGAACAGCATACAAAGTTCGAGTTGGTGCTGTGGGG
  GACTCTGGTTTTGTGAGTTCAGAGGATGTGTTTGAGACAGGCCCAGCGATGGCAAGCCGG
  CAGGTGGATATTGCAACTCAGGGCTGGTTCATTGGTCTGATGTGTGCTGTTGCTCTCCTT
  ATCTTAATTTTGCTGATTGTTTGCTTCATCAGAAGAAACAAGGGTGGTAAATATCCAGTT
  AAAGAAAAGGAAGATGCCCATGCTGACCCTGAAATCCAGCCTATGAAGGAAGATGATGGG
  ACATTTGGAGAATACAGTGATGCAGAAGACCACAAGCCTTTGAAAAAAGGAAGTCGAACT
  CCTTCAGACAGGACTGTGAAAAAAGAAGATAGTGACGACAGCCTAGTTGACTATGGAGAA
  GGGGTTAATGGCCAGTTCAATGAGGATGGCTCCTTTATTGGACAATACAGTGGTAAGAAA
  GAGAAAGAGCCGGCTGAAGGAAACGAAAGCTCAGAGGCACCTTCTCCTGTCAACGCCATG
  AATTCCTTTGTTTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001037132
• Insert Size: 6700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037132.1, NP_001032209.1
• RefSeq Size: 6659 bp
• RefSeq ORF: 3915 bp
• Locus ID: 4897
• UniProt ID: Q92823
• Cytogenetics: 7q31.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longest isoform (A).