TJP1 (NM_003257) Human Untagged Clone

CAT#: SC127835

TJP1 (untagged)-Human tight junction protein 1 (zona occludens 1) (TJP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TJP1
• Synonyms: ZO-1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003257, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCGCCAGAGCTGCGGCCGCCAAGAGCACAGCAATGGAGGAAACAGCTATATGGGAACAACATACAG
  TGACGCTTCACAGGGCTCCTGGATTTGGATTTGGAATTGCAATATCTGGTGGACGAGATAATCCTCATTT
  TCAGAGTGGGGAAACGTCAATAGTGATTTCAGATGTGCTGAAAGGAGGACCAGCTGAAGGACAGCTACAG
  GAAAATGACCGAGTTGCAATGGTTAACGGAGTTTCAATGGATAATGTTGAACATGCTTTTGCTGTTCAGC
  AACTAAGGAAAAGTGGGAAAAATGCAAAAATTACAATTAGAAGGAAGAAGAAAGTTCAAATACCAGTAAG
  TCGTCCTGATCCTGAACCAGTATCTGATAATGAAGAAGATAGTTATGATGAGGAAATACATGATCCAAGA
  AGTGGCCGGAGTGGTGTGGTTAACAGAAGGAGTGAGAAGATTTGGCCGAGGGATAGAAGTGCAAGTAGAG
  AGAGGAGCTTGTCCCCGCGGTCAGACAGGCGGTCAGTGGCTTCCAGCCAGCCTGCTAAACCTACTAAAGT
  CACACTGGTGAAATCCCGGAAAAATGAAGAATATGGTCTTCGATTGGCAAGCCATATATTTGTTAAGGAA
  ATTTCACAAGATAGTTTGGCAGCAAGAGATGGCAATATTCAAGAAGGTGATGTTGTATTGAAGATAAATG
  GTACTGTGACAGAAAATATGTCATTGACAGATGCAAAGACATTGATAGAAAGGTCTAAAGGCAAATTAAA
  AATGGTAGTTCAAAGAGATGAACGGGCTACGCTATTGAATGTCCCTGATCTTTCTGACAGCATCCACTCT
  GCTAATGCCTCTGAGAGAGACGACATTTCAGAAATTCAGTCACTGGCATCAGATCATTCTGGTCGATCAC
  ACGATAGGCCTCCCCGCCGCAGCCGGTCACGATCTCCTGACCAGCGGTCAGAGCCTTCTGATCATTCCAG
  GCACTCGCCGCAGCAGCCAAGCAATGGCAGTCTCCGGAGTAGAGATGAAGAGAGAATTTCTAAACCTGGG
  GCTGTCTCAACTCCTGTAAAGCATGCTGATGATCACACACCTAAAACAGTGGAAGAAGTTACAGTTGAAA
  GAAATGAGAAACAAACACCTTCTCTTCCAGAACCAAAGCCTGTGTATGCCCAAGTTGGGCAACCAGATGT
  GGATTTACCTGTCAGTCCATCTGATGGTGTCCTACCTAATTCAACTCATGAAGATGGGATTCTTCGGCCC
  AGCATGAAATTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGGCTGGCTGGTGGAAATGATGTTG
  GAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTGCAGCCAAGGAAGGCTTAGAGGAAGGTGATCAAAT
  TCTCAGGGTAAACAACGTAGATTTTACAAATATCATAAGAGAAGAAGCCGTCCTTTTCCTGCTTGACCTC
  CCTAAAGGAGAAGAAGTGACCATATTGGCTCAGAAGAAGAAGGATGTTTATCGTCGCATTGTAGAATCAG
  ATGTAGGAGATTCTTTCTATATTAGAACCCATTTTGAATATGAAAAGGAATCTCCCTATGGACTTAGTTT
  TAACAAAGGAGAGGTGTTCCGTGTTGTGGATACCTTGTACAATGGAAAACTGGGCTCTTGGCTTGCTATT
  CGAATTGGTAAAAATCATAAGGAGGTAGAACGAGGCATCATCCCTAATAAGAACAGAGCTGAGCAGCTAG
  CCAGTGTACAGTATACACTTCCAAAAACAGCAGGCGGAGACCGTGCTGACTTCTGGAGATTCAGAGGTCT
  TCGCAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGAGGATTTGTCCGCTCAGCCTGTTCAAACAAAGTTT
  CCAGCTTATGAAAGAGTGGTTCTTCGAGAAGCTGGATTTCTGAGGCCTGTAACCATTTTTGGACCAATAG
  CTGATGTTGCCAGAGAAAAGCTGGCAAGAGAAGAACCAGATATTTATCAAATTGCAAAGAGTGAACCACG
  AGACGCTGGAACTGACCAACGTAGCTCTGGCATTATTCGCCTGCATACAATAAAGCAAATCATAGATCAA
  GACAAACATGCTTTATTAGATGTAACACCAAATGCAGTTGATCGTCTTAACTATGCCCAGTGGTATCCAA
  TTGTTGTATTTCTTAACCCTGATTCTAAGCAAGGAGTAAAAACAATGAGAATGAGGTTATGTCCAGAATC
  TCGGAAAAGTGCCAGGAAGTTATACGAGCGATCTCATAAACTTCGTAAAAATAATCACCATCTTTTTACA
  ACTACAATTAACTTAAATTCAATGAATGATGGTTGGTATGGTGCGCTGAAAGAAGCAATTCAACAACAGC
  AAAACCAGCTGGTATGGGTTTCCGAGGGAAAGGCGGATGGTGCTACAAGTGATGACCTTGATTTGCATGA
  TGATCGTCTGTCCTACCTGTCAGCTCCAGGTAGTGAATACTCAATGTATAGCACGGACAGTAGACACACT
  TCTGACTATGAAGACACAGACACAGAAGGCGGGGCCTACACTGATCAAGAACTAGATGAAACTCTTAATG
  ATGAGGTTGGGACTCCACCGGAGTCTGCCATTACACGGTCCTCTGAGCCTGTAAGAGAGGACTCCTCTGG
  AATGCATCATGAAAACCAAACATATCCTCCTTACTCACCACAAGCGCAGCCACAACCAATTCATAGAATA
  GACTCCCCTGGATTTAAGCCAGCCTCTCAACAGAAAGCAGAAGCTTCATCTCCAGTCCCTTACCTTTCGC
  CTGAAACAAACCCAGCATCATCAACCTCTGCTGTTAATCATAATGTAAATTTAACTAATGTCAGACTGGA
  GGAGCCCACCCCAGCTCCTTCCACCTCTTACTCACCACAAGCTGATTCTTTAAGAACACCAAGTACTGAG
  GCAGCTCACATAATGCTAAGAGATCAAGAACCATCATTGTCGTCGCATGTAGATCCAACAAAGGTGTATA
  GAAAGGATCCATATCCCGAGGAAATGATGAGGCAGAACCATGTTTTGAAACAGCCAGCCGTTAGTCACCC
  AGGGCACAGGCCAGACAAAGAGCCTAATCTGACCTATGAACCCCAACTCCCATACGTAGAGAAACAAGCC
  AGCAGAGACCTCGAGCAGCCCACATACAGATACGAGTCCTCAAGCTATACGGACCAGTTTTCTCGAAACT
  ATGAACATCGTCTGCGATACGAAGATCGCGTCCCCATGTATGAAGAACAGTGGTCATATTATGATGACAA
  ACAGCCCTACCCATCTCGGCCACCTTTTGATAATCAGCACTCTCAAGACCTTGACTCCAGACAGCATCCC
  GAAGAGTCCTCAGAACGAGGGTACTTTCCACGTTTTGAAGAGCCAGCCCCTCTGTCTTACGACAGCAGAC
  CACGTTACGAACAGGCACCTAGAGCATCCGCCCTGCGGCACGAAGAGCAGCCAGCTCCTGGGTATGACAC
  ACATGGTAGACTCAGACCGGAAGCCCAGCCCCACCCTTCAGCAGGGCCCAAGCCTGCAGAGTCCAAGCAG
  TATTTTGAGCAATATTCACGCAGTTACGAGCAAGTACCACCCCAAGGATTTACCTCTAGAGCAGGTCATT
  TTGAGCCTCTCCATGGTGCTGCAGCTGTCCCTCCGCTGATACCTTCATCTCAGCATAAGCCAGAAGCTCT
  GCCTTCAAACACCAAACCACTGCCTCCACCCCCAACTCAAACCGAAGAAGAGGAAGATCCAGCAATGAAG
  CCACAGTCTGTACTCACCAGAGTTAAGATGTTTGAAAACAAAAGATCTGCATCCTTAGAGACCAAGAAGG
  ATGTAAATGACACTGGCAGTTTTAAGCCTCCAGAAGTAGCATCTAAACCTTCAGGTGCTCCCATCATTGG
  TCCCAAACCCACTTCTCAGAATCAATTCAGTGAACATGACAAAACTCTGTACAGGATCCCAGAACCTCAA
  AAACCTCAACTGAAGCCACCTGAAGATATTGTTCGGTCCAATCATTATGACCCTGAAGAAGATGAAGAAT
  ATTATCGAAAACAGCTGTCATACTTTGACCGAAGAAGTTTTGAGAATAAGCCTCCTGCACACATTGCCGC
  CAGCCATCTCTCCGAGCCTGCAAAGCCAGCGCATTCTCAGAATCAATCAAATTTTTCTAGTTATTCTTCA
  AAGGGAAAGCCTCCTGAAGCTGATGGTGTGGATAGATCATTTGGCGAGAAACGCTATGAACCCATCCAGG
  CCACTCCCCCTCCTCCTCCATTGCCCTCGCAGTATGCCCAGCCATCTCAGCCTGTCACCAGCGCGTCTCT
  CCACATACATTCTAAGGGAGCACATGGTGAAGGTAATTCAGTGTCATTGGATTTTCAGAATTCCTTAGTG
  TCCAAACCAGACCCACCTCCATCTCAGAATAAGCCAGCAACTTTCAGACCACCAAACCGAGAAGATACTG
  CTCAGGCAGCTTTCTATCCCCAGAAAAGTTTTCCAGATAAAGCCCCAGTTAATGGAACTGAACAGACTCA
  GAAAACAGTCACTCCAGCATACAATCGATTCACACCAAAACCATATACAAGTTCTGCCCGACCATTTGAA
  CGCAAGTTTGAAAGTCCTAAATTCAATCACAATCTTCTGCCAAGTGAAACTGCACATAAACCTGACTTGT
  CTTCAAAAACTCCCACTTCTCCAAAAACTCTTGTGAAATCGCACAGTTTGGCACAGCCTCCTGAGTTTGA
  CAGTGGAGTTGAAACTTTCTCTATCCATGCAGAGAAGCCTAAATATCAAATAAATAATATCAGCACAGTG
  CCTAAAGCTATTCCTGTGAGTCCTTCAGCTGTGGAAGAGGATGAAGATGAAGATGGTCATACTGTGGTGG
  CCACAGCCCGAGGCATATTTAACAGCAATGGGGGCGTGCTGAGTTCCATAGAAACTGGTGTTAGTATAAT
  TATCCCTCAAGGAGCCATTCCCGAAGGAGTTGAGCAGGAAATCTATTTCAAGGTCTGCCGGGACAACAGC
  ATCCTTCCACCTTTAGATAAAGAGAAAGGTGAAACACTGCTGAGTCCTTTGGTGATGTGTGGTCCCCATG
  GCCTCAAGTTCCTGAAGCCTGTGGAGCTGCGCTTACCACACTGTGATCCTAAAACCTGGCAAAACAAGTG
  TCTTCCCGGAGATCCAAATTATCTCGTTGGAGCAAACTGTGTTTCTGTCCTTATTGACCACTTTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003257 unedited
  GGCTTTTGTATACGACTCCTATAGGCGGCCGCGAATTCGGCACGAGGGNANAAACTCGGC
  TCCAGTGAGGGTTCGGGGGCTGGAAGCCGGCTCTCAGCGGGTCGGGGCTTGGGGTGCCAC
  CTCCTGCTGGCCGGGAGCTGCTGTCTTTGGAGGAGTGGTTGGTCCCCGGCGAAACCCTGT
  AGTTTCGATCTGATGTCACTCCCTGCGGTATGCGCACGCCAGCGATAAGGCTTTGAGACT
  GCAAAACACTCCACTCAGCCTGTGAGGCGTAGTAGAGCACAGCAATGGAGGAAACAGCTA
  TATGGGAACAACATACAGTGACGCTTCACAGGGCTCCTGGATTTGGATTTGGAATTGCAA
  TATCTGGTGGACGAGATAATCCTCATTTTCAGAGTGGGGAAACGTCAATAGTGATTTCAG
  ATGTGCTGAAAGGAGGACCAGCTGAAGGACAGCTACAGGAAAATGACCGAGTTGCAATGG
  TTAACGGAGTTTCAATGGATAATGTTGAACATGCTTTTGCTGTTCAGCAACTAAGGAAAA
  GTGGGAAAAATGCAAAAATTACAATTAGAAGGAAGAAGAAAGTTCANATACCAGTAAGTC
  GTCCTGATCCTGAACCAGTATCTGATAATGAAGAAGATAGTTATGATGAGGAAATACATG
  ATCCAAGAAGTGGCCGGAGTGGTGTGGTTAACAGAAGGAGTGAGAAGATTTGGCCGAGGA
  TAGAAGTGCAAGTAGAGAGAGGAGCTTGTCCCCGCGGTCAGACAGGCGGTCAGTGGCTTC
  CAGCCAGCCTGCTAAACCCTACTAAGTCACACTGGTGAAATCCCGNANAAATGAGATATG
  GTCTTCGATTGGCAGCCATATATTGNTAAGGAAATTCACAAAAAGTTTGGGCAGCAGAGA
  TGGCATATTCAGAGGGGATGTGTATTGAGAATATGTACTGGACGGAAAATGTCATGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003257 unedited
  TTTAACANNTTTGACTTGNNACCGCGGCCGCTTACNANNGATCGGTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTATTTTAAAAATTTTATTTTGGGAGATTAGAATTTGAGATTTTTAAT
  AACGGCAAAAGAAATCAGTCACACCTAATGATTAACAGAATGTAGTGGTGTATTATCTAA
  ACAGAAATCGTGCTGATGTGCCATAATAAATTGTCTATTAGTAAAAAAAACACTTTAGGG
  CACAGCATTGTATCACAAATTACAGTAGGGATACTTTGCAAGAATTTAATCAAACTAGAG
  AATTCTGAGTAACTGTATCTTTTAAATGCAGCACTTAAAAATGTAACAACTCTGTGCATC
  CTTTTTCTTAAAAAAAATGACCTTGCATGTGTCATAGAAACGCTGCTTTATTGCTGCAGA
  GGTCAAAGTTCAAGGCTCAAGAGGTACAGGAGAGAATACAAAGGTAGCCTTTAGAAACGT
  GGTCTTGTTTATGTATAAAAAAGGTAAAGTTTATAAAAGTTAATTTACAAACCAAGAACA
  AAAGTGGTATGCACGCATTATGTACAAGCATCCCTTAAAACATCAAATTTTTCAAATGCA
  TAGCCCCGAAGAACCGAAAACCCCCCCCGGCCCCTTTGTCAAAAAAAAAAAAAAAGGGAA
  AGGAAAGGAAAGGAAAAATTTTCCCCAAATCCACCCACTATTTTTTTCTGGGGGGATAAC
  CATTTTTTGAAACCCCCCAAGGGCCCAAGGTCCTCCAGTTCTTGGCATGGACCCGTTTTT
  CAATAAATGCCCCAAAACCCTTTAAACCTTTGTTACCCAGGGGGGAATGGGCCCAAAAAT
  TTAAAAAACTGGGGGGTAGAGGCTTTCCCGCCGGGTAAAATTTTCTCGGGGGAAAAAGGG
  AGAAATA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003257
• Insert Size: 5000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003257.1, NP_003248.1
• RefSeq Size: 7171 bp
• RefSeq ORF: 5211 bp
• Locus ID: 7082
• UniProt ID: Q07157
• Cytogenetics: 15q13.1
• Domains: ZU5, PDZ, Guanylate_kin, GuKc
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Epithelial cell signaling in Helicobacter pylori infection, Gap junction, Tight junction, Vibrio cholerae infection
• Gene Summary: This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (1, also known as ZO-1alpha+) encodes isoform a, which is found in most epithelial cell junctions.