Leucyl tRNA synthetase (LARS) (NM_020117) Human Untagged Clone

CAT#: SC127382

LARS (untagged)-Human leucyl-tRNA synthetase (LARS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Leucyl tRNA synthetase
• Synonyms: hr025Cl; HSPC192; ILFS1; LARS; LEURS; LEUS; LFIS; LRS; PIG44; RNTLS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127382 sequence for NM_020117 edited (data generated by NextGen Sequencing)
  ATGGCGGAAAGAAAAGGAACAGCCAAAGTGGACTTTTTGAAGAAGATTGAGAAAGAAATC
  CAACAGAAATGGGATACTGAGAGAGTGTTTGAGGTCAATGCATCTAATTTAGAGAAACAG
  ACCAGCAAGGGCAAGTATTTTGTAACCTTCCCATATCCATATATGAATGGACGCCTTCAT
  TTGGGACACACGTTTTCTTTATCCAAATGTGAGTTTGCTGTAGGGTACCAGCGATTGAAA
  GGAAAATGTTGTCTGTTTCCCTTTGGCCTGCACTGTACTGGAATGCCTATTAAGGCATGT
  GCTGATAAGTTGAAAAGAGAAATAGAGCTGTATGGTTGCCCCCCTGATTTTCCAGATGAA
  GAAGAGGAAGAGGAAGAAACCAGTGTTAAAACAGAAGATATAATAATTAAGGATAAAGCT
  AAAGGAAAAAAGAGTAAAGCTGCTGCTAAAGCTGGATCTTCTAAATACCAGTGGGGCATT
  ATGAAATCCCTTGGYCTGTCTGATGAAGAGATAGTAAAATTTTCTGAAGCAGAACATTGG
  CTTGATTATTTCCCGCCACTGGCTATTCAGGATTTAAAAAGAATGGGTTTGAAGGTAGAC
  TGGCGTCGTTCCTTCATCACCACTGATGTTAATCCTTACTATGATTCMTTTGTCAGATGG
  CAATTTTTAACATTAAGAGAAAGAAACAAAATTAAATTTGGGAAGCGGTATACAATTTAC
  TCTCCGAAAGATGGACAGCCTTGCATGGATCATGATAGACAAACTGGAGAGGGTGTTGGA
  CCTCAGGAATATACTTTACTCAAATTGAAGGTGCTTGAGCCATACCCATCTAAATTAAGT
  GGCCTGAAAGGTAAAAATATTTTCTTGGTGGCTGCTACTCTCAGACCTGAGACCATGTTT
  GGGCAGACAAATTGTTGGGTTCGTCCTGATATGAAGTACATTGGATTTGAGACGGTGAAT
  GGTGATATATTCATCTGTACCCAAAAAGCAGCCAGGAATATGTCATACCAGGGCTTTACC
  AAAGACAATGGCGTGGTGCCTGTTGTTAAGGAATTAATGGGGGAGGAAATTCTTGGTGCA
  TCACTTTCTGCACCTTTAACATCATACAAGGTGATCTATGTTCTCCCAATGCTAACTATT
  AAGGAGGATAAAGGCACTGGTGTGGTTACAAGTGTTCCTTCCGACTCCCCTGATGATATT
  GCTGCCCTCAGAGACTTGAAGAAAAAGCAAGCCTTACGAGCAAAATATGGAATTAGAGAT
  GACATGGTCTTGCCATTTGAGCCGGTGCCAGTCATTGAAATCCCAGGTTTTGGAAATCTT
  TCTGCTGTAACCATTTGTGATGAGTTGAAAATTCAGAGCCAGAATGACCGGGAAAAACTT
  GCAGAAGCAAAGGAGAAGATATATCTAAAAGGATTTTATGAGGGTATCATGTTGGTGGAT
  GGATTTAAAGGACAGAAGGTTCAAGATGTAAAGAAGACTATTCAGAAAAAGATGATTGAC
  GCTGGAGATGCACTTATTTACATGGAACCAGAGAAACAAGTGATGTCCAGGTCGTCAGAT
  GAATGTGTTGTGGCTCTGTGTGACCAGTGGTACTTGGATTATGGAGAAGAGAATTGGAAG
  AAACAGACATCTCAGTGCTTGAAGAACCTGGAAACATTCTGTGAGGAGACCAGGAGGAAT
  TTTGAAGCCACCTTAGGTTGGCTACAAGAACATGCTTGCTCAAGAACTTATGGTCTAGGC
  ACTCACYTGCCTTGGGATGAGCAGTGGCTGATTGAATCACTTTCTGACTCCACTATTTAC
  ATGGCATTTTACACAGTTGCACACCTATTGCAGGGGGGTAACTTGCATGGACAGGCAGAG
  TCTCCGCTGGGCATTAGACCGCAACAGATGACCAAGGAAGTTTGGGATTATGTTTTCTTC
  AAGGAGGCTCCATTTCCTAAGACTCAGATTGCAAAGGAAAAATTAGATCAGTTAAAGCAG
  GAGTTTGAATTCTGGTATCCTGTTGATCTTCGCGTCTCTGGCAAGGATCTTGTTCCAAAT
  CATCTTTCATATTACCTTTATAATCATGTGGCTATGTGGCCGGAACAAAGTGAYAAATGG
  CCTACAGCTGTGAGAGCAAATGGACATCTCCTCCTGAACTCTGAGAAGATGTCAAAATCC
  ACAGGCAACTTCCTCACTTTGACCCAAGCTATTGACAAATTTTCAGCAGATGGAATGCGT
  TTGGCTCTGGCTGATGCTGGTGACACTGTAGAAGATGCCAACTTTGTGGAAGCCATGGCA
  GATGCAGGTATTCTCCGTCTGTACACCTGGGTAGAGTGGGTGAAAGAAATGGTTGCCAAC
  TGGGACAGCCTAAGAAGTGGTCCTGCCAGCACTTTCAATGATAGAGTTTTTGCCAGTGAA
  TTGAATGCAGGAATTATAAAAACAGATCAAAACTATGAAAAGATGATGTTTAAAGAAGCT
  TTGAAAACAGGGTTTTTTGAGTTTCAGGCCGCAAAAGATAAGTACCGTGAATTGGCTGTG
  GAAGGGATGCACAGAGAACTTGTGTTCCGGTTTATTGAAGTTCAGACACTTCTCCTCGCT
  CCATTCTGTCCACATTTGTGTGAGCACATCTGGACACTCCTGGGAAAGCCTGACTCAATT
  ATGAATGCTTCATGGCCTGTGGCAGGTCCTGTKRATGAAGTTTTAATACACTCCTCACAG
  TATCTTATGGAAGTAACACATGACCTTAGACTACGACTCAAGAACTATATGATGCCAGCT
  AAAGGGAAGAAGACYGACAAACAACCCCTGCAGAAGCCCTCACATTGCACCATCTATGTG
  GCAAAGAACTATCCACCTTGGCAACATACCACCCTGTCTGTTCTACGTAAACACTTTGAG
  GCCAATAACGGAAAACTGCCTGACAACAAAGTCATTGCTAGTGAACTAGGCAGTATGCCA
  GAACTGAAGAAATACATGAAGAAAGTCATGCCATTTGTTGCCATGATTAAGGAAAATCTG
  GAGAAGATGGGGCCTCGTATTCTGGATTTGCAATTAGAATTTGATGAAAAGGCTGTGCTT
  ATGGAGAATATAGTCTATCTGACTAATTCGCTTGAGCTAGAACACATAGAAGTCAAGTTT
  GCCTCCGAAGCAGAAGATAAAATCAGGGAAGACTGCTGTCCTGGGAAACCACTTAATGTT
  TTTAGAATAGAACCTGGTGTGTCCGTTTCTCTGGTGAATCCCCAGCCATCCAATGGCCAC
  TTCTCAACCAAAATTGAAATCAGGCAAGGAGATAACTGTGATTCCATAATCAGGCGTTTA
  ATGAAAATGAATCGAGGAATTAAAGACCTTTCCAAAGTGAAACTGATGAGATTTGATGAT
  CCACTGTTGGGGCCTCGACGAGTTCCTGTCCTGGGAAAGGAGTACACCGAGAAGACCCCC
  ATTTCTGAGCATGCTGTTTTCAATGTGGACCTCATGAGCAAGAAAATTCATCTGACTGAG
  AATGGGATAAGGGTGGATATTGGCGATACAATAATCTATCTGGTTCATTAA
  
  Clone variation with respect to NM_020117.9
  495 c=>y;648 a=>m;1747 c=>y;2094 c=>y;2673 t=>k;2674 a=>r;2775 t=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020117 unedited
  TGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGTTTCGTGGGAGGCCT
  GGCATCTCCGAGAGGGAGGGGAAAGGTAACCACTCCTTTGTGGAGGTCGCCAGGGTCATT
  GTCGTGGATTTGCACAGTCGGCTGGGCGGTGCAATGGCGGAAAGAAAAGGAACAGCCAAA
  GTGGACTTTTTGAAGAAGATTGAGAAAGAAATCCAACAGAAATGGGATACTGAGAGAGTG
  TTTGAGGTCAATGCATCTAATTTAGAGAAACAGACCAGCAAGGGCAAGTATTTTGTAACC
  TTCCCATATCCATATATGAATGGACGCCTTCATTTGGGACACACGTTTTCTTTATCCAAA
  TGTGAGTTTGCTGTAGGGTACCAGCGATTGAAAGGAAAATGTTGTCTGTTTCCCTTTGGC
  CTGCACTGTACTGGAATGCCTATTAAGGCATGTGCTGATAAGTTGAAAAGAGAAATAGAG
  CTGTATGGTTGCCCCCCTGATTTTCCAGATGAAGAAGAGGAAGAGGAAGAAACCAGTGTT
  AAAACAGAAGATATAATAATTAAGGATAAAGCTAAAGGAAAAAAGAGTAAAGCTGCTGCT
  AAAGCTGGATCTTCTAAATACCAGTGGGGCATTATGAAATCCCTTTGGTCTGTCTGATGA
  AGAGATAGTAAAATTTTCTGAAGCAGAACATTGGCTTGATTATTTCCCGCCACTGGCTAT
  TCAGGATTAAAAAAAATGGGTTTTGAAGGTAGACTGGGGCGTCGTTCCTTCATCACCACT
  GATGGTAATCCCTACTATGATTCCTTTGGNCAGATGGGCATTTTTACATTAAGAGAAAGA
  AACAAATTAATTTGGGNAAAGCGGTATACATTTACTCTTCGAAGATGGACANNCTGCATG
  GACATGATAAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020117 unedited
  TGTCTATGGCCCGCGGGCCGCAATCTAGNGTCGAGTTTTTTTTTTTTTTTTTTCAGCATG
  ACCTCTATTTTATTGATTGATTGATGGATTGATTGAGACAGAGTTTCACAGCTGGAGTGC
  AATGGCACGGTTTCAGCTCACTGCAACCTCGGTCTCCCAGGTTCAAGCGATTCTCCTGCC
  TCACACTCCCAAGCAGCTGGGATTACAGGTACCCGCCACCATGCCAAGCTAATTTTTGTA
  TTTTTAGTAGAGACGGGGTTTCACCATGTTGGTCAGGCTGGTCTCAAACTCCTGACCTCA
  GGTGACTGCCCACCTTGGCCTCCCAAAGTGCTGGGATTAAATAGGTGTGAGCCACCACAC
  CCGACCAGCATGACCTTTAAACACAATTGGACTTAAGACAAATTTAGTAAATGCAGATTT
  CTGGGGGGGAAGGGGGGAATCTTCTTTTAAAATGCCCATATTTAGTGTAGGTGAAATATA
  CTGCTTATAAACTATTGATATTAGGCTTGAAGAAGCCATTATTGGTTCTTAGTTGAATAA
  TTAAAGCTTTAAAAAGTCTTATAAAATGCAGGACCTATTTGTTCTTTATACAAAATACTT
  AGTAATTGAAGTCAAGTCAAAATATTCTCATTAGTTCTTTTAAATCATCATGAAGTTCTC
  TTCATTTAAAAAAAACAAGCTGCTAATTTACTACTTTTCTTTTTTTAAAAAAATACAGCT
  AACTCCATAAATGAATATACTATAAAAAGCTGTTAGGTCCACCTTACCCTTCATCCAAGA
  NTAAAGCACACCTCATAAAAAAGCCTACTGACACTTTTATGTTCATCTTAAAAACAGAAC
  TCCTAGGAAATACACAATTTGTTTTTAAAAAGAGTTGGGCAAAACACTTTTCCTGCACCC
  CCAAGGAAGGCAAAAAATCTATTAGTCCAGGATCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020117
• Insert Size: 4730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020117.8, NP_064502.8
• RefSeq Size: 4248 bp
• RefSeq ORF: 3531 bp
• Locus ID: 51520
• UniProt ID: Q9P2J5
• Cytogenetics: 5q32
• Domains: tRNA-synt_1
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
• Gene Summary: This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. A mutation in this gene was found in affected individuals with infantile liver failure syndrome 1. Alternatively spliced transcript variants of this gene have been observed. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.