SKP1 (NM_006930) Human Untagged Clone

CAT#: SC126980

SKP1 (untagged)-Human S-phase kinase-associated protein 1 (SKP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SKP1
• Synonyms: EMC19; OCP-II; OCP2; p19A; SKP1A; TCEB1L
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126980 sequence for NM_006930 edited (data generated by NextGen Sequencing)
  ATGCCTTCAATTAAGTTGCAGAGTTCTGATGGAGAGATATTTGAAGTTGATGTGGAAATT
  GCCAAACAATCTGTGACTATTAAGACCATGTTGGAAGATTTGGGAATGGATGATGAAGGA
  GATGATGACCCAGTTCCTCTACCAAATGTGAATGCAGCAATATTAAAAAAGGTCATTCAG
  TGGTGCACCCACCACAAGGATGACCCTCCTCCTCCTGAAGATGATGAGAACAAAGAAAAG
  CGAACAGATGATATCCCTGTTTGGGACCAAGAATTCCTGAAAGTTGACCAAGGAACACTT
  TTTGAACTCATTCTGGCTGCAAACTACTTAGACATCAAAGGTTTGCTTGATGTTACATGC
  AAGACTGTTGCCAATATGATCAAGGGGAAAACTCCTGAGGAGATTCGCAAGACCTTCAAT
  ATCAAAAATGACTTTACTGAAGAGGAGGAAGCCCAGGTAGGTAGCACACAGTTTTGTCTT
  TGA
  
  Clone variation with respect to NM_006930.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006930 unedited
  TGTATACGACTCATATAGGCGGCNCGCGAATTCGCACGAGGGCTGTAGTGGCTTCGTCTT
  CGGTTTTTCTCTTCCTTCGCTAACGCCTCCCGGCTCTCGTCAGCCTCCCGCCGGCCGTCT
  CCTTAACACCGAACACCATGCCTTCAATTAAGTTGCAGAGTTCTGATGGAGAGATATTTG
  AAGTTGATGTGGAAATTGCCAAACAATCTGTGACTATTAAGACCATGTTGGAAGATTTGG
  GAATGGATGATGAAGGAGATGATGACCCAGTTCCTCTACCAAATGTGAATGCAGCAATAT
  TAAAAAAGGTCATTCAGTGGTGCACCCACCACAAGGATGACCCTCCTCCTCCTGAAGATG
  ATGAGAACAAAGAAAAGCGAACAGATGATATCCCTGTTTGGGACCAAGAATTCCTGAAAG
  TTGACCAAGGAACACTTTTTGAACTCATTCTGGCTGCAAACTACTTAGACATCAAAGGTT
  TGCTTGATGTTACATGCAAGACTGTTGCCAATATGATCAAGGGGAAAACTCCTGAGGAGA
  TTCGCAAGACCTTCAATATCAAAAATGACTTTACTGAAGAGGAGGAAGCCCAGGTAGGTA
  GCACACAGTTTTGTCTTTGATCACATGCTCTTAAAAAGGGAGTAACACCTATAACATGCC
  AGCAAAAGAGGTGACTGTATCAAGTAGTCTTGATACTTTAGGGATAATATGTACTTAATG
  TGTCTTAAACATACTCTTACTGGTCTGTCTTTATGAATCTGAACACTTAGATGATACTGC
  ATTTTAAGCATAAGAACATTAGTTTTAATGAGAAGAGCTCTTGNTATGTATAAACAGTAC
  TAGTTNTGCACCCTGCACTTCTGTATAGACATACTATCTGACTCAAAAAGCAGCCTAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006930 unedited
  CGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTATAGAGAACCCA
  CAGTTCAGTTTTATTCAGAGCAAAGAAAAAAGAAACTTTCCATCATACTAAAAAAAACTT
  GGCCGTAAGGTTTGGGATCTGTGCTCAAACTACACATGCAATGAGGACAATATTCTGCTA
  ATACAATTGACTTGCTGCTGCATTTGTCTACTGTTTGTCTAATATTAACAATTATAAACA
  GAGCAGTGCAACTAGTATTTGGAACAATCCTTACAGTGTTACAGTGTCAGGCACAACATT
  TCACTTCTCTTCACACCACTGGTTCTCTTTGCGTACCTAAAAGAGATGGATATAGGGAAG
  TACCTTAACTTGAGTAGTCTTTCCTAAGACTTATAAATACTACCTACTGATTCATCGATA
  GCCAGTGAGTTGAGTCAGAAGAACAGATGGAAAATTAGAACAACACCAGGTTAAGACTAT
  ATTTCCGGATTTCCTTTCTTTGCCTCCCATGGTTTTTATTCTGCCCTGCTGAAATTATGA
  ACAAAGCAAAGGCAATGGGCAATTTACTAAAGTTGACAGAGCAAATTCAGTTAATTCATT
  CACATTATTTCAGTCTGTAGTCATGTCAACAAAATCATTCTGATCTATTCTTTCATTTCC
  TCCCCTCATTAAGTTGCTCTAAAGTACCCTAGTATATACTTCTCTCTAGGGCCTGCTTTT
  CTGATGTCAGATATGTATGGTCTATACAGAAAGTTGCAGGTGCCAAAACTAGTAACTGTT
  AATACATTAANCAAGAGCTCTTCTCATTGAAAACTAATNGNTCTTATGCTTAAATGCANG
  TATCATCTAGTGTTCAGATTCATAAGACAGAACAGTAGAGTTGTTTAAGAACATTNAGTA
  CATATATCCCTAAGTATCAGACTACTGAACAGCACCTCTTTGCTGGATGNTATAGTGTAC
  N
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006930
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006930.2, NP_008861.2
• RefSeq Size: 2172 bp
• RefSeq ORF: 483 bp
• Locus ID: 6500
• UniProt ID: P63208
• Cytogenetics: 5q31.1
• Domains: Skp1
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway
• Gene Summary: This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript variant but encodes the shorter isoform (a).