CK1 epsilon (CSNK1E) (NM_001894) Human Untagged Clone

CAT#: SC126810

CSNK1E (untagged)-Human casein kinase 1, epsilon (CSNK1E), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CK1 epsilon
• Synonyms: CKIe; CKIepsilon; HCKIE
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126810 sequence for NM_001894 edited (data generated by NextGen Sequencing)
  ATGGAGCTACGTGTGGGGAACAAGTACCGCCTGGGACGGAAGATCGGGAGCGGGTCCTTC
  GGAGATATCTACCTGGGTGCCAACATCGCCTCTGGTGAGGAAGTCGCCATCAAGCTGGAG
  TGTGTGAAGACAAAGCACCCCCAGCTGCACATCGAGAGCAAGTTCTACAAGATGATGCAG
  GGTGGCGTGGGGATCCCGTCCATCAAGTGGTGCGGAGCTGAGGGCGACTACAACGTGATG
  GTCATGGAGCTGCTGGGGCCTAGCCTCGAGGACCTGTTCAACTTCTGTTCCCGCAAATTC
  AGCCTCAAGACGGTGCTGCTCTTGGCCGACCAGATGATCAGCCGCATCGAGTATATCCAC
  TCCAAGAACTTCATCCACCGGGACGTCAAGCCCGACAACTTCCTCATGGGGCTGGGGAAG
  AAGGGCAACCTGGTCTACATCATCGACTTCGGCCTGGCCAAGAAGTACCGGGACGCCCGC
  ACCCACCAGCACATTCCCTACCGGGAAAACAAGAACCTGACCGGCACGGCCCGCTACGCT
  TCCATCAACACGCACCTGGGCATTGAGCAAAGCCGTCGAGATGACCTGGAGAGCCTGGGC
  TACGTGCTCATGTACTTCAACCTGGGCTCCCTGCCCTGGCAGGGGCTCAAAGCAGCCACC
  AAGCGCCAGAAGTATGAACGGATCAGCGAGAAGAAGATGTCAACGCCCATCGAGGTCCTC
  TGCAAAGGCTATCCCTCCGAATTCTCAACATACCTCAACTTCTGCCGCTCCCTGCGGTTT
  GACGACAAGCCCGACTACTCTTACCTACGTCAGCTCTTCCGCAACCTCTTCCACCGGCAG
  GGCTTCTCCTATGACTACGTCTTTGACTGGAACATGCTGAAATTCGGTGCAGCCCGGAAT
  CCCGAGGATGTGGACCGGGAGCGGCGAGAACACGAACGCGAGGAGAGGATGGGGCAGCTA
  CGGGGGTCCGCGACCCGAGCCCTGCCCCCTGGCCCACCCACGGGGGCCACTGCCAACCGG
  CTCCGCAGTGCCGCCGAGCCCGTGGCTTCCACGCCAGCCTCCCGCATCCAGCCGGCTGGC
  AATACTTCTCCCAGAGCGATCTCGCGGGTCGACCGGGAGAGGAAGGTGAGTATGAGGCTG
  CACAGGGGTGCGCCCGCCAACGTCTCCTCCTCAGACCTCACTGGGCGGCAAGAGGTCTCC
  CGGATCCCAGCCTCACAGACAAGTGTGCCATTTGACCATCTCGGGAAGTGA
  
  Clone variation with respect to NM_001894.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001894 unedited
  NGGTCGCAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGGCAGG
  AGGCCGGGAGGAGGGAGGCGGCGGCGGCGGCGGCGGCGGCGGCGAGAGCCCAGAGCCAGA
  GCCCGGCCGGGGCCGAGCGGAGCGCGGCGGCGGCGGCGGCGGCGGCGGCTGGGCCGGGAG
  AGGCTGGCGCGCCGGGCGGCTCCGCGAATCCTCCGGCATCCGCCCCGGCGGGCCGCCCCC
  GCCCGCGGCAGCCCCCCGAGCAGTGGCCCGGCATCGGCGCCTTCCCGGCGGGCAAGAGTG
  AGCCATGGAGCTACGTGTGGGGAACAAGTACCGCCTGGGACGGAAGATCGGGAGCGGGTC
  CTTCGGAGATATCTACCTGGGTGCCAACATCGCCTCTGGTGAGGAAGTCGCCATCAAGCT
  GGAGTGTGTGAAGACAAAGCACCCCCAGCTGCACATCGAGAGCAAGTTCTACAAGATGAT
  GCAGGGTGGCGTGGGGATCCCGTCCATCAAGTGGTGCGGAGCTGAGGGCGACTACAACGT
  GATGGTCATGGAGCTGCTGGGGCCTAGCCTCGAGGACCTGTTCAACTTCTGTTCCCGCAA
  ATTCAGCCTCAAGACGGTGCTGCTCTTGGCCGACCAGATGATCAGCCGCATCGAGTATAT
  CCACTCCAAGAACTTCATCCACCGGGACGTCAAGCCCCGACACTTTCCTCATGGGGCTGG
  GGAAGAANGGGCACCTGGTCTACATCATCGACTTCGGCCTGGCCAAGAAGTACCGGGACG
  CCCGCACCACCAGCACATTCCCTACCGGGAAAACAAGAACCCTGACCGGCACGGCCCGCT
  ACGCTTCCCATCACACGCACCTGGGCATTGAGCANAGCCGTCCAGATGACCTGGAGAGCC
  TNGGCTACGTGCTCATGTACTTCAACCTGGGCTCCCTGCCCTGGCAGGNGCTCAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001894 unedited
  GTCATTGGGCGATGTGCAACTTCCCAGGNCCAGGNAAAGCACTGGGNGAGGGTCACAGGG
  CATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAG
  TTTTTTTTTTTTTTTTTTTTTTAAAGAAAATACAGTNGAAGACACTAAGCAAACACTGGT
  CCAATGGGGGCTCTCCTCACTTCCCGAGATGGTCAAATGGCACACTTGTCTGTGAGGCTG
  GGATCCGGGAGACCTCTTGCCGCCCAGTGAGGTCTGAGGAGGAGACGTTGGCGGGCGCAC
  CCCTGTGCAGCCTCATACTCACCTTCCTCTCCCGGTCGACCCGCGAGATCGCTCTGGGAG
  AAGTATTGCCAGCCGGCTGGATGCGGGAGGCTGGCGTGGAAGCCACGGGCTCGGCGGCAC
  TGCGGAGCCGGTTGGCAGTGGCCCCCGTGGGTGGGCCAGGGGGCAGGGCTCGGGTCGCGG
  ACCCCCGTAGCTGCCCCATCCTCTCCTCGCGTTCGTGTTCTCGCCGCTCCCGGTCCAAAT
  CCTCGGGATTCCGGGCTGCACCCTAATTCATCCTGGGGCCCGTCACAGGGAAGGGGCAGG
  GGGCTGGCAAGAAAGGGCCCCCATGAATTTCACCATGTTCCAATCAAAAACGTAGTCATA
  GAAAAACCCCTGCCGGTGGAATAGGGTGCGGAAGAACTCTACTTACGTAAAAGTATCCGG
  CTTTGGTCTCCAAACCCCAGGAGGCGGCAAAAATTGGAGTATGTTTAAAATTCCGGAGGA
  AAACCTTTGCAAAAGAACCTCAATGGGCGTTGAAATCTTTCTTCCCCCGCATCCCCTTCA
  ATATTTTGGCTCCTTGGCGGCCTGTGTTGCACCCCCCGCCACAGGCAGCGAAGCCCAATT
  TTAGAATACATAGAAACCCTGTACCCCACCGCTCTTTGGCACCACACGAAGGCATCTGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001894
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001894.4, NP_001885.1
• RefSeq Size: 2670 bp
• RefSeq ORF: 1251 bp
• Locus ID: 1454
• UniProt ID: P49674
• Cytogenetics: 22q13.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Circadian rhythm - mammal, Hedgehog signaling pathway, Wnt signaling pathway
• Gene Summary: The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.