HLA DP (HLA-DPA1) (BC009956) Human Untagged Clone

CAT#: SC126195

HLA (untagged)-Human major histocompatibility complex, class II, DP alpha 1, mRNA (cDNA clone MGC:14114 IMAGE:4309471), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLA DP
• Synonyms: DP(W3); DP(W4); HLA-DP1A; HLADP; HLASB; PLT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC009956 edited
  GCTCACAGTCATCAATTATAGACCCCACAACATGCGCCCTGAAGACAGAATGTTCCATAT
  CAGAGCTGTGATCTTGAGAGCCCTCTCCTTGGCTTTCCTGCTGAGTCTCCGAGGAGCTGG
  GGCCATCAAGGCGGACCATGTGTCAACTTATGCCGCGTTTGTACAGACCCATAGACCAAC
  AGGGGAGTTTATGTTTGAATTTGATGAAGATGAGCAGTTCTATGTGGATCTGGATAAAAA
  GGAGACCGTCTGGCATCTGGAGGAGTTTGGCCGAGCCTTTTCCTTTGAGGCTCAGGGCGG
  GCTGGCTAACATTGCTATATTGAACAACAACTTGAATACCTTGATCCAGCGTTCCAACCA
  CACTCAGGCCGCCAATGATCCCCCTGAGGTGACCGTGTTTCCCAAGGAGCCTGTGGAGCT
  GGGCCAGCCCAACACCCTCATCTGCCACATTGACAGGTTCTTCCCACCAGTGCTCAACGT
  CACATGGCTGTGCAATGGGGAGCCAGTCACTGAGGGTGTCGCTGAGAGCCTCTTCCTGCC
  CAGAACAGATTACAGCTTCCACAAGTTCCATTACCTGACCTTTGTGCCCTCAGCAGAGGA
  CGTCTATGACTGCAGGGTGGAGCACTGGGGCTTGGACCAGCCGCTCCTCAAGCACTGGGA
  GGCCCAAGAGCCAATCCAGATGCCTGAGACAACGGAGACTGTGCTCTGTGCCCTGGGCCT
  GGTGCTGGGCCTAGTGGGCATCATCGTGGGCACCGTCCTCATCATAAAGTCTCTGCGTTC
  TGGCCATGACCCCCGGGCCCAGGGGCCCCTGTGAAATACTGTAAAGGTGACAAAATATCT
  GAACAGAAGAGGACTTAGGAGAGATCTGAACTCCAGCTGCCCTACAAACTCCGTCACAGC
  TTTTCTTCTCACTTCATGTGAAAACTACCCCAGTGGCTGACTGAATTGCTGACCCTTCAA
  GCTCTGTCCTTATCCGTTACCTTAAAGCAGTCATTCCTTAGTAAAGTTTCCAACAAATAG
  AAATTAATGAATAAATGTTGACACTTTGATAGCACTGATATGGAGATTATCCTTTCATTG
  AGCCTTTTATCCTCTGGTCTTCTTTGAAAAACCCCTCACTGTCACCTTCCTGAGAATACT
  CTAAGACCAATAAATACTTCAGTATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  A
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC009956 unedited
  NCGGACCGCGAAATTCGGCACGAGGGCTCACAGTCATCAATTATAGNACCCCACAACATG
  CGCCCTGAAGACAGAATGTTCCATATCAGAGCTGTGATCTTGAGAGCCCTCTCCTTGGCT
  TTCCTGCTGAGTCTCCGAGGAGCTGGGGCCATCAAGGCGGACCATGTGTCAACTTATGCC
  GCGTTTGTACAGACCCATAGACCAACAGGGGAGTTTATGTTTGAATTTGATGAAGATGAG
  CCAGTCTATGTGGATCTGGGTAAAAAAGAAAACCGCCTGGGCATTTGAAGGATTTTGGCC
  CAAACCTTTTCCTTTGGGGCTTAAGGGGGGGGCTGGGCAAAACTTGTTTTTTTTGAAAAC
  ACACTTTAAAACCTTTGTACCCCGGTTTCACCCCCCCCTTGTGGGCCCCAAAAAACCCCC
  CCCGGGGGAACCTTTTTCCCCAAAAAAATTTGGGTTTGGTGCCCCCCCCCCCCCCCTTTT
  TTCAAAAAAGAGGGGGTTCCCCCCCCCGGGGAAAAAAAATATTTTTTTGGGGGGGGGCCA
  CCCCCCCAGGGGGGGGGGGAAAAACCCCCTCTCCCCCCCCAAAAAAAAAAAAACCACCCC
  CAACNNNNNTCCTTTTCTCCNTTTCCCCCCCCCCCCCCNANAAAAAAAAAAATTTATTTT
  TTTGTGGGGGGGGGGGGGTGTTTTTTTTTTTTTTCCCACCCCCCCCCCCTCCCTTTCTGG
  GGGGGGGGGGGGAGGGGGGCCGACACCCCCCTTTTTTATCCCCAATATAACATCATCCCC
  CCTCCTTNTTCTTTTTTTTCTGGCCAGCGGGGGG
  
• Restriction Sites: Please inquire
• ACCN: BC009956
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC009956.2, AAH09956.1
• RefSeq Size: 1201 bp
• RefSeq ORF: 780 bp
• Locus ID: 3113
• Cytogenetics: 6p21.32
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq, Jul 2008]