HLA (BC024269) Human Untagged Clone

CAT#: SC125953

HLA (untagged)-Human major histocompatibility complex, class II, DR beta 1, mRNA (cDNA clone MGC:39133 IMAGE:4852401), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLA
• Synonyms: DRB1; DRw10; HLA-DR1B; HLA-DRB; SS1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC024269 edited
  CTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCTGAAGTT
  CCCTGGAGGCTCCTGCATGGCAGCTCTGACAGTGACACTGATGGTGCTGAGCTCCCCACT
  GGCTTTGGCTGGGGACACCCGACCACGTTTCTTGGAGCAGGTTAAACATGAGTGTCATTT
  CTTCAACGGGACGGAGCGGGTGCGGTTCCTGGACAGATACTTCTATCACCAAGAGGAGTA
  CGTGCGCTTCGACAGCGACGTGGGGGAGTACCGGGCGGTGACGGAGCTGGGGCGGCCTAG
  CGCCGAGTACTGGAACAGCCAGAAGGACCTCCTGGAGCAGAGGCGGGCCGCGGTGGACAC
  CTACTGCAGACACAACTACGGGGTTGTGGAGAGCTTCACAGTGCAGCGGCGAGTCTATCC
  TGAGGTGACTGTGTATCCTGCAAAGACCCAGCCCCTGCAGCACCACAACCTCCTGGTCTG
  CTCTGTGAATGGTTTCTATCCAGGCAGCATTGAAGTCAGGTGGTTCCGGAACGGCCAGGA
  AGAGAAGACTGGGGTGGTGTCCACAGGCCTGATCCAGAATGGAGACTGGACCTTCCAGAC
  CCTGGTGATGCTGGAAACAGTTCCTCGGAGTGGAGAGGTTTACACCTGCCAAGTGGAGCA
  CCCAAGCCTGACGAGCCCTCTCACAGTGGAATGGAGAGCACGGTCTGAATCTGCACAGAG
  CAAGATGCTGAGTGGAGTCGGGGGCTTCGTGCTGGGCCTGCTCTTCCTTGGGGCCGGGCT
  GTTCATCTACTTCAGGAATCAGAAAGGACACTCTGGACTTCAGCCAACAGGATTCCTGAG
  CTGAAGTGAAGATGACCACATTCAAGGAAGAACCTTCTGCCCCAGCTTTGCAGGATGAAA
  CACTTCCCCGCTTGGCTCTCATTCTTCCACAAGAGAGACCTTTCTCCGGACCTGGTTGCT
  ACTGGTTCAGCAGCTCTGCAGAAAATGTCCTCCCTTGTGGCTGCCTCAGCTCGTACCTTT
  GGCCTGAAGTCCCAGCATTAATGGCAGCCCCTCATCTTCCAAGTTTTGTGCTCCCCTTTA
  CCTAATGCTTCCTGCCTCCCATGCATCTGTACTCCTGCTGTGCCACAAACACATTACATT
  ATTAAATGTTTCTCAAACAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC024269 unedited
  GCAGTTCATATTTGTATACGACTCATATAGGCGGCCGCGTAATTCGCACGAGGCTCACCT
  GCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCTGAAGTTCCCTGGA
  GGCTCCTGCATGGCAGCTCTGACAGTGACACTGATGGTGCTGAGCTCCCCACTGGCTTTG
  GCTGGGGACACCCGACCACGTTTCTTGGAGCAGGTTAAACATGAGTGTCATTTCTTCAAC
  GGGACGGAGCGGGTGCGGTTCCTGGACAGATACTTCTATCACCAAGAGGAGTACGTGCGC
  TTCGACAGCGACGTGGGGGAGTACCGGGCGGTGACGGAGCTGGGGCGGCCTAGCGCCGAG
  TACTGGAACAGCCAGAAGGACCTCCTGGAGCAGAGGCGGGCCGCGGTGGACACCTACTGC
  AGACACAACTACGGGGTTGTGGAGAGCTTCACAGTGCAGCGGCGAGTCTATCCTGAGGTG
  ACTGTGTATCCTGCAAAGACCCAGCCCCTGCAGCACCACAACCTCCTGGTCTGCTCTGTG
  AATGGTTTCTATCCAGGCAGCATTGAAGTCAGGTGGTTCCGGAACGGCCAGGAAGAGAAG
  ACTGGGGTGGTGTCCACAGGCCTGATCCAGAATGGAGACTGGACCTTCCAGACCCTGGTG
  ATGCTGGAAACAGTTCCTCGGAGTGGAGAGGTTTACACCTGCCAAGTGGAGCACCCAAGC
  CTGACGAGCCCTCTCACAGTGGAATGGAGAGCACGGTCTGAATCTGCACAGAGCAAGATG
  CTGAGTGGAGTCGGGGGCTTCGTGCTGGGCCTGCTCTTCCTGGGGCCGGGCTGTTCATTT
  CTTCAGGAATAAAAAGACACTCTGGACTCACCCAAAGGATTCTGAGCGAAATGAAGAGAC
  CACTTC
  
• Restriction Sites: NotI-NotI
• ACCN: BC024269
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC024269.1, AAH24269.1
• RefSeq Size: 1177 bp
• Locus ID: 3123
• Cytogenetics: 6p21.32
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Hematopoietic cell lineage, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and some alleles have increased frequencies associated with certain diseases or conditions. For example, DRB1*1302 has been related to acute and chronic hepatitis B virus persistence. There are multiple pseudogenes of this gene. [provided by RefSeq, Jul 2020]