DARC (ACKR1) (NM_002036) Human Untagged Clone

CAT#: SC125243

DARC (untagged)-Human Duffy blood group, chemokine receptor (DARC), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DARC
• Synonyms: CCBP1; CD234; DARC; DARC/ACKR1; Dfy; FY; GPD; GpFy; WBCQ1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125243 sequence for NM_002036 edited (data generated by NextGen Sequencing)
  ATGGGGAACTGTCTGCACAGGGCGGAGCTCTCCCCCTCAACTGAGAACTCAAGTCAGCTG
  GACTTCGAAGATGTATGGAATTCTTCCTATGGTGTGAATGATTCCTTCCCAGATGGAGAC
  TATGGTGCCAACCTGGAAGCAGCTGCCCCCTGCCACTCCTGTAACCTGCTGGATGACTCT
  GCACTGCCCTTCTTCATCCTCACCAGTGTCCTGGGTATCCTAGCTAGCAGCACTGTCCTC
  TTCATGCTTTTCAGACCTCTCTTCCGCTGGCAGCTCTGCCCTGGCTGGCCTGTCCTGGCA
  CAGCTGGCTGTGGGCAGTGCCCTCTTCAGCATTGTGGTGCCCGTCTTGGCCCCAGGGCTA
  GGTAGCACTCGCAGCTCTGCCCTGTGTAGCCTGGGCTACTGTGTCTGGTATGGCTCAGCC
  TTTGCCCAGGCTTTGCTGCTAGGGTGCCATGCCTCCCTGGGCCACAGACTGGGTGCAGGC
  CAGGTCCCAGGCCTCACCCTGGGGCTCACTGTGGGAATTTGGGGAGTGGCTGCCCTACTG
  ACACTGCCTGTCACCCTGGCCAGTGGTGCTTCTGGTGGACTCTGCACCCTGATATACAGC
  ACGGAGCTGAAGGCTTTGCAGGCCACACACACTGTAGCCTGTCTTGCCATCTTTGTCTTG
  TTGCCATTGGGTTTGTTTGGAGCCAAGGGGCTGAAGAAGGCATTGGGTATGGGGCCAGGC
  CCCTGGATGAATATCCTGTGGGCCTGGTTTATTTTCTGGTGGCCTCATGGGGTGGTTCTA
  GGACTGGATTTCCTGGTGAGGTCCAAGCTGTTGCTGTTGTCAACATGTCTGGCCCAGCAG
  GCTCTGGACCTGCTGCTGAACCTGGCAGAAGCCCTGGCAATTTTGCACTGTGTGGCTACG
  CCCCTGCTCCTCGCCCTATTCTGCCACCAGGCCACCCGCACCCTCTTGCCCTCTCTGCCC
  CTCCCTGAAGGATGGTCTTCTCATCTGGACACCCTTGGAAGCAAATCCTAG
  
  Clone variation with respect to NM_002036.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002036 unedited
  ACTATAGGGCGGCCGCGAATTCGGCACCAGGTGCAGGCAGTGGGCGTGGGGTAAGGCTTC
  CTGATGCCCCCTGTCCCTGCCCAGAACCTGATGGCCCTCATTAGTCCTTGGCTCTTATCT
  TGGAAGCACAGGCGCTGACAGCCGTCCCAGCCCTTCTGTCTGCGGGCCTGAACCAAACGG
  TGCCATGGGGAACTGTCTGCACAGGGCGGAGCTCTCCCCCTCAACTGAGAACTCAAGTCA
  GCTGGACTTCGAAGATGTATGGAATTCTTCCTATGGTGTGAATGATTCCTTCCCAGATGG
  AGACTATGGTGCCAACCTGGAAGCAGCTGCCCCCTGCCACTCCTGTAACCTGCTGGATGA
  CTCTGCACTGCCCTTCTTCATCCTCACCAGTGTCCTGGGTATCCTAGCTAGCAGCACTGT
  CCTCTTCATGCTTTTCAGACCTCTCTTCCGCTGGCAGCTCTGCCCTGGCTGGCCTGTCCT
  GGCACAGCTGGCTGTGGGCAGTGCCCTCTTCAGCATTGTGGTGCCCGTCTTGGCCCCAGG
  GCTAGGTAGCACTCGCAGCTCTGCCCTGTGTAGCCTGGGCTACTGTGTCTGGTATGGCTC
  AGCCTTTGCCCAGGCTTTGCTGCTAGGGTGCCATGCCTCCCTGGGCCACAGACTGGGTGC
  AGGCCANGTCCCANGCCTCACCCTGGGGCTCACTGTGGGAATTTTGGGAGTGGCTGCCCT
  ACTGACACTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002036 unedited
  GAGGGTGCGGGTGGCCTGGTGGCAGAATAGGGCGAGGAGCAGGGGCGTAGCCACACAGTG
  CAAAATTGCCAGGGCTTCTGCCAGGTTCAGCAGCAGGTCCAGAGCCTGCTGGGCCAGACA
  TGTTGACAACAGCAACAGCTTGGACCTCACCAGGAAATCCAGTCCTAGAACCACCCCATG
  AGGCCACCAGAAAATAAACCAGGCCCACAGGATATTCATCCAGGGGCCTGGCCCCATACC
  CAATGCCTTCTTCAGCCCCTTTGGCTCCAAACAAACCCAATGGCAACAAGACAAAGATGG
  CAAGACAGGCTACAGTGTGTGTGGCCTGCAAAGCCTTCAGCTCCGTGCTGTATATCAGGG
  TGCAGAGTCCACCAGAAGCACCACTGGCCAGGGTGACAGGCAGTGTCAGTAGGGCAGCCA
  CTCCCCAAATTCCCACAGTGAGCCCCAGGGTGAGGCCTGGGACCTGGCCTGCACCCAGTC
  TGTGGCCCAGGGAGGCATGGCAACCCTAGCAGCAAAGCCTGGGCAAAGGCTGAGCCATAC
  CAGACACAGTAGCCCAGGCTACACAGGGCAGAGCTGCGAGTGCTACCTAGCCCTGGGGCC
  AAGACGGGCACCACAATGCTGAAGAGGGCACTGCCCACAGCCAGCTGTGCCAGGACAGGC
  CAGCCAGGGCAGAGCTGCCAGCGGAAGAGAGGTCTGAAAAGCATGAAGAGGACAGTGCTG
  CTAGCTAGGATACCCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002036
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002036.2, NP_002027.2
• RefSeq Size: 1317 bp
• RefSeq ORF: 1011 bp
• Locus ID: 2532
• UniProt ID: Q16570
• Cytogenetics: 1q23.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: The protein encoded by this gene is a glycosylated membrane protein and a non-specific receptor for several chemokines. The encoded protein is the receptor for the human malarial parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a.