PAM (NM_138822) Human Untagged Clone

CAT#: SC124251

PAM (untagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAM
• Synonyms: PAL; PHM
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_138822, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGGCCGCGTCCCTAGCCTGCTAGTTCTCCTTGTTTTTCCAAGCAGCTGTTTGGCTTTCCGAAGCC
  CACTTTCTGTCTTTAAGAGGTTTAAAGAAACTACCAGACCATTTTCCAATGAATGTCTTGGTACCACCAG
  ACCCGTAGTTCCTATTGATTCATCAGATTTTGCATTGGATATTCGCATGCCTGGGGTTACACCTAAACAG
  TCCGATACATACTTCTGCATGTCTATGCGAATACCAGTGGATGAGGAAGCCTTCGTGATTGACTTCAAGC
  CTCGAGCCAGCATGGATACTGTCCATCACATGTTACTTTTTGGATGCAATATGCCTTCATCCACTGGAAG
  TTACTGGTTTTGTGATGAAGGAACCTGTACAGATAAAGCCAATATTCTGTATGCCTGGGCGAGAAATGCT
  CCCCCTACCCGGCTCCCCAAAGGTGTTGGATTCAGAGTTGGAGGAGAGACTGGAAGTAAATACTTTGTAC
  TACAGGTACACTATGGGGATATTAGTGCTTTTAGAGATAATAACAAGGACTGTTCTGGTGTGTCCTTACA
  CCTCACACGTCTGCCACAGCCTTTAATTGCTGGCATGTACCTTATGATGTCTGTTGACACTGTTATCCCA
  GCAGGAGAAAAAGTGGTGAATTCTGACATTTCATGCCATTATAAAAATTATCCAATGCATGTCTTTGCCT
  ATAGAGTTCACACTCACCATTTAGGTAAGGTAGTAAGTGGATACAGAGTAAGAAATGGACAGTGGACACT
  GATTGGACGGCAGAGCCCTCAGCTGCCACAGGCTTTCTACCCTGTGGGGCATCCAGTTGATGTAAGTTTT
  GGTGACCTACTGGCTGCAAGATGTGTATTCACTGGTGAAGGAAGGACAGAAGCCACACACATTGGTGGCA
  CGTCTAGTGATGAAATGTGCAACTTATACATTATGTATTACATGGAAGCCAAGCATGCAGTTTCTTTCAT
  GACCTGTACCCAGAATGTAGCTCCAGATATGTTCAGAACCATACCACCAGAGGCCAACATTCCAATTCCC
  GTGAAGTCTGATATGGTTATGATGCATGAACATCATAAAGAAACAGAATATAAAGATAAGATTCCTTTAC
  TACAGCAGCCAAAACGAGAAGAAGAAGAAGTGTTAGACCAGGGTGATTTCTATTCACTACTTTCCAAGCT
  GCTAGGAGAAAGGGAAGATGTTGTTCATGTGCACAAATATAATCCTACAGAAAAGGCAGAATCAGAGTCA
  GACCTGGTAGCTGAGATTGCAAATGTAGTCCAAAAAAAGGATCTTGGTCGATCTGATGCCAGAGAGGGTG
  CAGAACATGAGAGGGGTAATGCTATTCTTGTCAGAGACAGAATTCACAAATTCCACAGACTAGTATCTAC
  CTTGAGGCCACCAGAGAGCAGAGTTTTCTCATTACAGCAGCCCCCACCTGGTGAAGGCACCTGGGAACCA
  GAACACACAGGAGATTTCCACATGGAAGAGGCACTGGATTGGCCTGGAGTATACTTGTTACCAGGCCAGG
  TTTCTGGGGTGGCTCTAGACCCTAAGAATAACCTGGTGATTTTCCACAGAGGTGACCATGTCTGGGATGG
  AAACTCGTTTGACAGCAAGTTTGTTTACCAGCAAATAGGACTCGGACCAATTGAAGAAGACACTATTCTT
  GTCATAGATCCAAATAATGCTGCAGTACTCCAGTCCAGTGGAAAAAATCTGTTTTACTTGCCACATGGCT
  TGAGTATAGATAAAGATGGGAATTATTGGGTCACAGACGTGGCTCTCCATCAGGTGTTCAAACTGGATCC
  AAACAATAAAGAAGGCCCTGTATTAATCCTGGGAAGGAGCATGCAACCAGGCAGTGACCAGAATCACTTC
  TGTCAACCCACTGATGTGGCTGTGGATCCAGGCACTGGAGCCATTTATGTATCAGATGGTTACTGCAACA
  GCAGGATTGTGCAGTTTTCACCAAGTGGAAAGTTCATCACACAGTGGGGAGAAGAGTCTTCAGGGAGCAG
  TCCTCTGCCAGGCCAGTTCACTGTTCCTCACAGCTTGGCTCTTGTGCCTCTTTTGGGCCAATTATGTGTG
  GCAGACCGGGAAAATGGTCGGATCCAGTGTTTTAAAACTGACACCAAAGAATTTGTGAGAGAGATTAAGC
  ATTCATCATTTGGAAGAAATGTATTTGCAATTTCATATATACCAGGCTTGCTCTTTGCAGTGAATGGGAA
  GCCTCATTTTGGGGACCAAGAACCTGTACAAGGATTTGTGATGAACTTTTCCAATGGGGAAATTATAGAC
  ATCTTCAAGCCAGTGCGCAAGCACTTTGATATGCCTCATGATATTGTTGCATCTGAAGATGGGACTGTGT
  ACATTGGAGATGCTCATACCAACACCGTGTGGAAGTTCACCTTGACTGAGAAATTGGAACATCGATCAGT
  TAAAAAGGCTGGCATTGAGGTCCAGGAAATCAAAGGAAAGGGAAGTGGAGGCTTAAACCTTGGTAATTTC
  TTTGCAAGCCGTAAGGGCTACAGTCGAAAAGGGTTTGACCGGCTTAGCACTGAGGGCAGTGACCAAGAGA
  AAGAGGATGATGGAAGTGAATCAGAAGAGGAGTATTCAGCACCTCTGCCTGCGCTCGCACCTTCCTCCTC
  CTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138822 unedited
  GTCAGCATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACCAGCCGTGGTCGCG
  CAGGTTCTGAATGATGACTGACGCGGGTTTGGGTGATACCCCTCACAGCCCCTGTCATTC
  CGGAGTGATAAGGCACCCGCGCGTCTAGCCCCAGCGCCAGGGCACGCGAGCGGCGCTGGA
  GGGAGGAAAGCTTCCGCCTGCGGGCCGGACAAAAGTCCCGCCTGCCCACGGCTTTTTGCC
  CGCCGCTCGTGACCGAGACGCCTCGCCGCGGCCAGCTCGCTGCTCTCGCTGGCGGATGGT
  GTGTGGCCGCCGCAAGACGCCCGCCGTGCCCGGGCCATGAAGTAGCGGCTGCTGGCGGCG
  CCGCTGCCCAACCGCCAGCCCCAGCCCCGCGCTGCGCTGCCCGGTCCTCTCCCGGCGGGG
  TCGTATCGGCGTGGACATGGCTGGCCGCGTCCCTAGCCTGCTAGTTCTCCTTGTTTTTCC
  AAGCAGCTGTTTGGCTTTCCGAAGCCCACTTTCTGTCTTTAAGAGGTTTAAAGAAACTAC
  CAGACCATTTTCCAATGAATGTCTTGGTACCACCAGACCCGTAGTTCCTATTGATTCATC
  AGATTTTGCATTGGATATTCGCATGCCTGGGGTTACACCTAAACAGTCCGATACATACTT
  CTGCATGTCTATGCGAATACCAGTGGATGAGGAAGCCTTCGTGATTGACTTCAAGCCTCG
  AGCCAGCATGGATACTGTCCATCACATGTTACTTTNTGGATGCAATATGCCTTCATCCAC
  TGGAAGTTACTGGTTTTGTGATGAAAGAACCTGTACAGATAAAGCCAATATTCTGTATGC
  CTGGGCGATGAATGCTNCCCCTACCCCGCTCCCAAGTGTTGGATTCAAACTGGAGGAAGA
  CTGGAAGTAATACTTGTACTACAGTCACTATGGGNATATTATGCTTTAAGATATAACAGA
  CTGTNTGGGGTGTCTTACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_138822
• Insert Size: 3950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138822.1, NP_620177.1
• RefSeq Size: 5098 bp
• RefSeq ORF: 2664 bp
• Locus ID: 5066
• UniProt ID: P19021
• Cytogenetics: 5q21.1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (4) lacks two alternate in-frame exons in the 3' coding region compared to variant 5. The encoded isoform (d) is shorter than isoform e. This isoform (d) may undergo proteolytic processing similar to isoform e. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.