HLA (BC001023) Human Untagged Clone

CAT#: SC123794

HLA (untagged)-Human cDNA clone MGC:2284 IMAGE:3345335, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLA
• Synonyms: DRB1; DRw10; HLA-DR1B; HLA-DR3B; HLA-DRB; SS1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC001023 edited
  GTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGC
  ATGGTGTGTCTGAAGCTCCCTGGAGGCTCCTGCATGGCAGCTCTGACAGTGACACTGATG
  GTGCTGAGCTCCCCACTGGCTTTGGCTGGGGACACCCAACCACGTTTCCTGTGGCAGGGT
  AAGTATAAGTGTCATTTCTTCAACGGGACGGAGCGGGTGCAGTTCCTGGAAAGACTCTTC
  TATAACCAGGAGGAGTTCGTGCGCTTCGACAGCGACGTGGGGGAGTACCGGGCGGTGACG
  GAGCTAGGGCGGCCTGTCGCCGAGTCCTGGAACAGCCAGAAGGACATCCTGGAGGACAGG
  CGGGGCCAGGTGGACACCGTGTGCAGACACAACTACGGGGTTGGTGAGAGCTTCACAGTG
  CAGCGGCGAGTCCATCCTGAGGTGACTGTGTATCCTGCCAAGACTCAGCCCCTGCAGCAC
  CACAACCTCCTGGTCTGCTCTGTGAGTGGTTTCTATCCAGGCAGCATTGAAGTCAGGTGG
  TTCCGGAACGGCCAGGAAGAGAAGGCTGGGGTGGTGTCCACAGGCCTGATCCAGAATGGA
  GACTGGACCTTCCAGACCCTGGTGATGCTGGAAACAGTTCCTCGGAGTGGAGAAGTTTAC
  ACCTGCCAAGTGGAGCACCCAAGTGTGATGAGCCCTCTCACAGTGGAATGGAGAGCACGG
  TCTGAATCTGCACAGAGCAAGATGCTGAGTGGAGTCGGGGGCTTTGTGCTGGGCCTGCTC
  TTCCTTGGGGCCGGGTTGTTCATCTACTTCAGGAATCAGAAAGGACACTCTGGACTTCAG
  CCAACAGGATTCCTGAGCTGAAGTGAAGATGACCACATTCAAGGAAAAACCTTCTGCCCC
  AGCTTTGCAGGATGAAACACTTCCCCGCTTGGCTCTCATTCTTCCACAAGAGAGACCTTT
  CTCCGGACCTGGTTGCTACTGGTTCAGCAACTCTGCAGAAAATGTCCTCCCCTGTGGCTG
  CCTCAGCTCATGCCTTTGGCCTGAAGTCCCAGCATTGATGGCAGCCCCTCATCTTCCAAG
  TTTTGTGCTCCCCTTTACCTAACGCTTCCTGCCTCCCATGCATCTGTACTCCTCCTGTGC
  CACAAACACATTACATTATTAAATGTTTCTCAAACATGGAAAAAAAAAAAAAAAAAAAAA
  AAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC001023 unedited
  NNNAAGGTCAAAATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGTTCT
  CCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGT
  GTGTCTGAAGCTCCCTGGAGGCTCCTGCATGGCAGCTCTGACAGTGACACTGATGGTGCT
  GAGCTCCCCACTGGCTTTGGCTGGGGACACCCAACCACGTTTCCTGTGGCAGGGTAAGTA
  TAAGTGTCATTTCTTCAACGGGACGGAGCGGGTGCAGTTCCTGGAAAGACTCTTCTATAA
  CCAGGAGGAGTTCGTGCGCTTCGACAGCGACGTGGGGGAGTACCGGGCGGTGACGGAGCT
  AGGGCGGCCTGTCGCCGAGTCCTGGAACAGCCAGAAGGACATCCTGGAGGACAGGCGGGG
  CCAGGTGGACACCGTGTGCAGACACAACTACGGGGTTGGTGAGAGCTTCACAGTGCAGCG
  GCGAGTCCATCCTGAGGTGACTGTGTATCCTGCCAAGACTCAGCCCCTGCAGCACCACAA
  CCTCCTGGTCTGCTCTGTGAGTGGTTTCTATCCAGGCAGCATTGAAGTCAGGTGGTTCCG
  GAACGGCCAGGAAGAGAAGGCTGGGGTGGTGTCCACAGGCCTGATCCAGAATGGAGACTG
  GACCTTCCAGACCCTGGTGATGCTGGAAACAGTTCCTCGGAGTGGAGAAGTTTACACCTG
  CCAAGTGGAGCACCCAAGTGTGATGAGCCCTCTCACAGTGGAATGGAGAGCACGGTCTGA
  ATCTGCACAGAGCAAGATGCTGAGTGGAGTCGGGGGCTTTGTGCTGGGCCTGCTCTTCCT
  GGGGCCGGGTTGGTCATCTACTTCAGGAATCAGAAAGACACTCTGGACTTCACCAACAGG
  ATCCTGAGCTG
  
• Restriction Sites: Please inquire
• ACCN: BC001023
• Insert Size: 1212 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC001023.2, AAH01023.1
• RefSeq Size: 1203 bp
• RefSeq ORF: 798 bp
• Locus ID: 3125
• Cytogenetics: 6p21.3
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Hematopoietic cell lineage, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. There are multiple pseudogenes of this gene. [provided by RefSeq, Feb 2020]