Cytokeratin 18 (KRT18) (NM_000224) Human Untagged Clone

CAT#: SC120062

KRT18 (untagged)-Human keratin 18 (KRT18), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cytokeratin 18
• Synonyms: CK-18; CYK18; K18
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000224 edited
  GAATTCGGCACGAGGGTCGTCCGCAAAGCCTGAGTCCTGTCCTTTCTCTCTCCCCGGACA
  GCATGAGCTTCACCACTCGCTCCACCTTCTCCACCAACTACCGGTCCCTGGGCTCTGTCC
  AGGCGCCCAGCTACGGCGCCCGGCCGGTCAGCAGCGCGGCCAGCGTCTATGCAGGCGCTG
  GGGGCTCTGGTTCCCGGATCTCCGTGTCCCGCTCCACCAGCTTCAGGGGCGGCATGGGGT
  CCGGGGGCCTGGCCACCGGGATAGCCGGGGGTCTGGCAGGAATGGGAGGCATCCAGAACG
  AGAAGGAGACCATGCAAAGCCTGAACGACCGCCTGGCCTCTTACCTGGACAGAGTGAGGA
  GCCTGGAGACCGAGAACCGGAGGCTGGAGAGCAAAATCCGGGAGCACTTGGAGAAGAAGG
  GACCCCAGGTCAGAGACTGGAGCCATTACTTCAAGATCATCGAGGACCTGAGGGCTCAGA
  TCTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATGCCCGTCTTG
  CTGCTGATGACTTTAGAGTCAAGTATGAGACAGAGCTGGCCATGCGCCAGTCTGTGGAGA
  ACGACATCCATGGGCTCCGCAAGGTCATTGATGACACCAATATCACACGACTGCAGCTGG
  AGACAGAGATCGAGGCTCTCAAGGAGGAGCTGCTCTTCATGAAGAAGAACCACGAAGAGG
  AAGTAAAAGGCCTACAAGCCCAGATTGCCAGCTCTGGGTTGACCGTGGAGGTAGATGCCC
  CCAAATCTCAGGACCTCGCCAAGATCATGGCAGACATCCGGGCCCAATATGACGAGCTGG
  CTCGGAAGAACCGAGAGGAGCTAGACAAGTACTGGTCTCAGCAGATTGAGGAGAGCACCA
  CAGTGGTCACCACACAGTCTGCTGAGGTTGGAGCTGCTGAGACGACGCTCACAGAGCTGA
  GACGTACAGTCCAGTCCTTGGAGATCGACCTGGACTCCATGAGAAATCTGAAGGCCAGCT
  TGGAGAACAGCCTGAGGGAGGTGGAGGCCCGCTACGCCCTACAGATGGAGCAGCTCAACG
  GGATCCTGCTGCACCTTGAGTCAGAGCTGGCACAGACCCGGGCAGAGGGACAGCGCCAGG
  CCCAGGAGTATGAGGCCCTGCTGAACATCAAGGTCAAGCTGGAGGCTGAGATCGCCACCT
  ACCGCCGCCTGCTGGAAGATGGCGAGGACTTTAATCTTGGTGATGCCTTGGACAGCAGCA
  ACTCCATGCAAACCATCCAAAAGACCACCACCCGCCGGATAGTGGATGGCAAAGTGGTGT
  CTGAGACCAATGACACCAAAGTTCTGAGGCATTAAGCCAGCAGAAGCAGGGTACCCTTTG
  GGGAGCAGGAGGCCAATAAAAAGTTCAGAGTTCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000224 unedited
  ATTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCGTCCGCAAAT
  CCTGAGTCCTGTCCTTTCTCTCTCCCCGGACAGCATGAGCTTCACCACTCGCTCCACCTT
  CTCCACCAACTACCGGTCCCTGGGCTCTGTCCAGGCGCCCAGCTACGGCGCCCGGCCGGT
  CAGCAGCGCGGCCAGCGTCTATGCAGGCGCTGGGGGCTCTGGTTCCCGGATCTCCGTGTC
  CCGCTCCACCAGCTTCAGGGGCGGCATGGGGTCCGGGGGCCTGGCCACCGGGATAGCCGG
  GGGTCTGGCAGGAATGGGAGGCATCCAGAACGAGAAGGAGACCATGCAAAGCCTGAACGA
  CCGCCTGGCCTCTTACCTGGACAGAGTGAGGAGCCTGGAGACCGAGAACCGGAGGCTGGA
  GAGCAAAATCCGGGAGCACTTGGAGAAGAAGGGACCCCAGGTCAGAGACTGGAGCCATTA
  CTTCAAGATCATCGAGGACCTGAGGGCTCAGATCTTCGCAAATACTGTGGACAATGCCCG
  CATCGTTCTGCAGATTGACAATGCCCGTCTTGCTGCTGATGACTTTAGAGTCAAGTATGA
  GACAGAGCTGGCCATGCGCCAGTCTGTGGAGAACGACATCCATGGGCTCCGCAAGGTCAT
  TGATGACACCAATATCACACGACTGCAGCTGGAGACAGAGATCGAGGCTCTCAAGGAGGA
  GCTGCTCTTCATGAAGAAGACCACGAAGAGGAAGTAAAAGGCCCTACAGCCCAGNATGCC
  AGCTCTGGGGGTGACCGTGGAGGTANATGCCCCCAATCTCAGGACCTCGCCAAGATCATG
  CAGACATCCGGCCCTATATGACGAGCTGGCTCGNAAGACCGAGAGGAGCTAGACAGTACT
  GGTCTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000224 unedited
  GGCCGCATCTATATCGAGTTTTTTTTTTTTTTTTTTGAACTCTGAACTTTTTATTGGCCT
  CCTGCTCCCCAAAGGTACCCTGCTTCTGCTGGCTTAATGCCTCAAAACTTTGGTGTCATT
  GGTCTCAAACACCACTTTGCCATCCACTATCCGGCGGGTGGTGGTCTTTTGGATGGTTTG
  CATGGAGTTGCTGCTGTCCAAGGCATCACCAAGATTAAAGTCCTCGCCATCTTCCAGCAG
  GCGGTGGTAGGTGGCGAACTCAGCCTCCAACTTGACCTTGATGTTCAGCAGGGTCTAATA
  CTACTGGGCCTGCCGCTGTTCATCTGCCCGGTTCTGTATCATCTCCGACCTCTGGGGCAA
  CACGATTCTCCATGTCTCGCTCCCTCGTTAGGACTAGCGGACATCATCTCTCTTCTTCGC
  TGCATCTTCCTCCCTACTTCAACTTCAATCACCTCAATATCAATTTTTTTCCTTACTACC
  ACTTCATTTACTTTACCTTCATAATTTTCCCTCTTCTTTCCCCTCATCATCCCCTCTTCT
  CCCTCTCGCTACTCCCTTAGTCTCTCTCTTCATTCTCACTATTACTTTTCTACCATCTCT
  ACCTCCCATCACCATTTTCTCCTTGCATCCTTTCACTTCTTTCCCCCACCCACTTACTCC
  TTCTAATCGCACACTTCAACAACCACCCTCCCCCTCCTCCCTCCTCATCTTCCCCTTCCT
  CCTACCCTCCCCGTCCTCCTCCCGTCCTCTTTTTGCATATCTTTCTTTCATAACTTTGTC
  CCACTACCCCCCCTTCTCTACCCCACTCTTCACCCTCACTTCCACCCTCTCTCAACCCCC
  CATCACCCCTGTACTTTCGTCCTAACTCTTGTCGTCAAACTGTTCCTCTCACCTTCGCTC
  CTTTATCGCCTCACTTTATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000224
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000224.2, NP_000215.1
• RefSeq Size: 1485 bp
• RefSeq ORF: 1293 bp
• Locus ID: 3875
• UniProt ID: P05783
• Cytogenetics: 12q13.13
• Domains: filament
• Protein Pathways: Pathogenic Escherichia coli infection
• Gene Summary: KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.