p35 (CDK5R1) (NM_003885) Human Untagged Clone

CAT#: SC117693

CDK5R1 (untagged)-Human cyclin-dependent kinase 5, regulatory subunit 1 (p35) (CDK5R1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: p35
• Synonyms: CDK5P35; CDK5R; NCK5A; p23; p25; p35; p35nck5a
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117693 sequence for NM_003885 edited (data generated by NextGen Sequencing)
  ATGGGCACGGTGCTGTCCCTGTCTCCCAGCTACCGGAAGGCCACGCTGTTTGAGGATGGC
  GCGGCCACCGTGGGCCACTATACGGCCGTACAGAACAGCAAGAACGCCAAGGACAAGAAC
  CTGAAGCGCCACTCCATCATCTCCGTGCTGCCTTGGAAGAGAATCGTGGCCGTGTCGGCC
  AAGAAGAAGAACTCCAAGAAGGTGCAGCCCAACAGCAGCTACCAGAACAACATCACGCAC
  CTCAACAATGAGAACCTGAAGAAGTCGCTGTCGTGCGCCAACCTGTCCACATTCGCCCAG
  CCCCCACCGGCCCAGCCGCCTGCACCCCCGGCCAGCCAGCTCTCGGGTTCCCAGACCGGG
  GGCTCCTCCTCAGTCAAGAAAGCCCCTCACCCTGCCGTCACCTCCGCAGGGACGCCCAAA
  CGGGTCATCGTCCAGGCGTCCACCAGTGAGCTGCTTCGCTGCCTGGGTGAGTTTCTCTGC
  CGCCGGTGCTACCGCCTGAAGCACCTGTCCCCCACGGACCCCGTGCTCTGGCTGCGCAGC
  GTGGACCGCTCGCTGCTTCTGCAGGGCTGGCAGGACCAGGGCTTCATCACGCCGGCCAAC
  GTGGTCTTCCTCTACATGCTCTGCAGGGATGTTATCTCCTCCGAGGTGGGCTCGGATCAC
  GAGCTCCAGGCCGTCCTGCTGACATGCCTGTACCTCTCCTACTCCTACATGGGCAACGAG
  ATCTCCTACCCGCTCAAGCCCTTCCTGGTGGAGAGCTGCAAGGAGGCCTTTTGGGACCGT
  TGCCTCTCTGTCATCAACCTCATGAGCTCAAAGATGCTGCAGATAAATGCCGACCCACAC
  TACTTCACACAGGTCTTCTCCGACCTGAAGAACGAGAGCGGCCAGGAGGACAAGAAGCGG
  CTCCTCCTAGGCCTGGATCGGTGA
  
  Clone variation with respect to NM_003885.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003885 unedited
  GGATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGGCGGCGGGGCC
  GCGGCGCGCATTGCGGAGGGCGCGGAGCGCAGGAGCTGCCGCCTGCCGGGCAGATCCAAG
  GGGGCAGCACGCTTCCCGGGAGCGCCCCCGCCTCCTCTCCGGGGCCGCCGCAGGCTCGGT
  GAGCGGTTTTATCCCTCCGGCCGGCAGGCTGGGCGCGCAGGGGCGCGAGCCCCCGCCCGG
  CGCGCAGCAGCACCATGGGCACGGTGCTGTCCCTGTCTCCCAGCTACCGGAAGGCCACGC
  TGTTTGAGGATGGCGCGGCCACCGTGGGCCACTATACGGCCGTACAGAACAGCAAGAACG
  CCAAGGACAAGAACCTGAAGCGCCACTCCATCATCTCCGTGCTGCCTTGGAAGAGAATCG
  TGGCCGTGTCGGCCAAGAAGAAGAACTCCAAGAAGGTGCAGCCCAACAGCAGCTACCAGA
  ACAACATCACGCACCTCAACAATGAGAACCTGAAGAAGTCGCTGTCGTGCGCCAACCTGT
  CCACATTCGCCCAGCCCCCACCGGCCCAGCCGCCTGCACCCCCGGCCAGCCAGCTCTCGG
  GTTCCCAGACCGGGGGCTCCTCCTCAGTCAAGAAAGCCCCTCACCCTGCCGTCACCTCCG
  CAGGGACGCCCAAACGGGTCATCGTCCAGGCGTCCACCAGTGAGCTGCTTCGCTGCCTGG
  GGTGAGTTTCTCTGCCGCCGGTGCTACCGCCTGAAGCACCTGTCCCCCACGGACCCCGTG
  CTCTGGCTGCGCAGCGTGGACCGCTCGCTGCTTCTGCAGGGCTGGGCAGACCAGGGCTTT
  ATCACGCCGCCCAAACGTGGTCTTTCTCTCAGCCTGGGAGGGGGATGNTATCTCTCCCCC
  AGTGGGGCTCGGATCACAGCCTCNCAGNCCGTNCTGCTGACATGCCTGTACCTCTCTATN
  CACTGGNNNNCACGAGATCTCTACCGCTAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003885 unedited
  ACCGCGGCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTTATGGATGGTCAGTGC
  ATTTTATTGAATCAGCACAGTACAAAAATAAATAAAAATAAGGGAAGGGGAATTAAATTA
  CAGCCAAACTGAGCTTCATGACTTTGTCAGATTATAAACCACACATACTCACAAACACAC
  TACACATACATACAAAATGAGACAAATATAAATTAATATTAACAATACCCACAGTTTGGT
  CAAAGAATAGCTACAGAAGAAATTGCACTAAAAAACCAACATACATCACACGTGTGTAAT
  TAGCAGTTTCAAATATACAGCTATGAATAATTCTGAGTGAAAAAAATGGCACATTTTCTT
  TTCATTGCAAGTTTAACTGTTGGAACAAAACTAAGTTCTAATAACTGCATGGAAAGAATA
  TATCAACCCTCCAGGTTTACAAGAAAAAGAGAAAAACCAAATCCTGAACTATTATTCCTG
  TGGCTTGTTCTGTGCATGGGTGTAATTTTCACAGCCATCTGTTCTAAGATTATTTTTCTT
  AAGACACAAAATGCTCCTGGTTTGCATGCACAATATCACCGTAAAAGCAACAATTAAAGA
  ATAAAGGCTCTTTTGTAAGTTTTTTTTTATTGTTGATCTAAAAGTAGTCTTCATAGCAGC
  ATGGCATATGGGTTCTTTGCTTGCACACTCAAGTATATCAGTTGTTCCTTTACACAATAC
  TGATGACCAGTGCAAGGGGAGATGACACCGACAGCTTTACACTGAGGGAACTAGAGAGTT
  CTTCCCTTATTCCCCACCACTTCCGATATGTCCACCTTCTCCGAAGATAAAAAAATAATA
  CGGAGAAAGAAAGGAATCAGACACGCACTGGGATCCTGTGGATGCAACGTTACTGCACAG
  TTCGCATCCAGGTTTCCGCAGCTGAAGTTGACCACACATGCGCCCNCTTCCGAGACTAGG
  ACTGAACCTCCTCTTNCTGCCACCAGGGCCTNCCACTCTGTCGCACGCGNAACAGATCTG
  ATCAAGCTGGGTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003885
• Insert Size: 4130 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003885.2, NP_003876.1
• RefSeq Size: 3870 bp
• RefSeq ORF: 924 bp
• Locus ID: 8851
• UniProt ID: Q15078
• Cytogenetics: 17q11.2
• Domains: CDK5_activator
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease
• Gene Summary: The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008]