CCBL1 (KYAT1) (NM_004059) Human Untagged Clone

CAT#: SC117604

CCBL1 (untagged)-Human cysteine conjugate-beta lyase, cytoplasmic (CCBL1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCBL1
• Synonyms: CCBL1; GTK; KAT1; KATI
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117604 sequence for NM_004059 edited (data generated by NextGen Sequencing)
  ATGGCCAAACAGCTGCAGGCCCGAAGGCTAGACGGGATCGACTACAACCCCTGGGTGGAG
  TTTGTGAAACTGGCCAGTGAGCATGACGTCGTGAACTTGGGCCAGGGCTTCCCGGATTTC
  CCACCACCAGACTTTGCCGTGGAAGCCTTTCAGCACGCTGTCAGTGGAGACTTCATGCTT
  AACCAGTACACCAAGACATTTGGTTACCCACCACTGACGAAGATCCTGGCAAGTTTCTTT
  GGGGAGCTGCTGGGTCAGGAGATAGACCCGCTCAGGAATGTGCTGGTGACTGTTGGTGGC
  TATGGGGCCCTGTTCACAGCCTTCCAGGCCCTGGTGGACGAAGGAGACGAGGTCATCATC
  ATCGAACCCTTTTTTGACTGCTACGAGCCCATGACAATGATGGCAGGGGGTCGTCCTGTG
  TTTGTGTCCCTGAAGCCGGGTCCCATCCAGAATGGAGAACTGGGTTCCAGCAGCAACTGG
  CAGCTGGACCCCATGGAGCTGGCCGGCAAATTCACATCACGCACCAAAGCCCTGGTCCTC
  AACACCCCCAACAACCCCCTGGGCAAGGTGTTCTCCAGGGAAGAGCTGGAGCTGGTGGCC
  AGCCTTTGCCAGCAGCATGACGTGGTGTGTATCACTGATGAAGTCTACCAGTGGATGGTC
  TACGACGGGCACCAGCACATCAGCATTGCCAGCCTCCCTGGCATGTGGGAACGGACCCTG
  ACCATCGGCAGCGCCGGCAAGACCTTCAGCGCCACTGGCTGGAAGGTGGGCTGGGTCCTG
  GGTCCAGATCACATCATGAAGCACCTGCGGACCGTGCACCAGAACTCCGTCTTCCACTGC
  CCCACGCAGAGCCAGGCTGCAGTAGCCGAGAGCTTTGAACGGGAGCAGCTGCTCTTCCGC
  CAACCCAGCAGCTACTTTGTGCAGTTCCCGCAGGCCATGCAGCGCTGCCGTGACCACATG
  ATACGTAGCCTACAGTCAGTGGGCCTGAAGCCCATCATCCCTCAGGGCAGCTACTTCCTC
  ATCACAGACATCTCAGACTTCAAGAGGAAGATGCCTGACTTGCCTGGAGCTGTGGATGAG
  CCCTATGACAGACGCTTCGTCAAGTGGATGATCAAGAACAAGGGCTTGGTGGCCATCCCT
  GTCTCCATCTTCTATAGTGTGCCACATCAGAAGCACTTTGACCACTATATCCGCTTCTGT
  TTTGTGAAGGATGAAGCCACGCTCCAGGCCATGGACGAGAAGCTGCGGAAGTGGAAGGTG
  GAACTCTAG
  
  Clone variation with respect to NM_004059.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004059 unedited
  TTACCCCCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG
  CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC
  GAATTCGGCACGAGGTGGGAACGAGGGGTGAAGCGGCCCAGGTGAGGCTCGCCATGGCCA
  AACAGCTGCAGGCCCGAAGGCTAGACGGGATCGACTACAACCCCTGGGTGGAGTTTGTGA
  AACTGGCCAGTGAGCATGACGTCGTGAACTTGGGCCAGGGCTTCCCGGATTTCCCACCAC
  CAGACTTTGCCGTGGAAGCCTTTCAGCACGCTGTCAGTGGAGACTTCATGCTTAACCAGT
  ACACCAAGACATTTGGTTACCCACCACTGACGAAGATCCTGGCAAGTTTCTTTGGGGAGC
  TGCTGGGTCAGGAGATAGACCCGCTCAGGAATGTGCTGGTGACTGTTGGTGGCTATGGGG
  CCCTGTTCACAGCCTTCCAGGCCCTGGTGGACGAAGGAGACGAGGTCATCATCATCGAAC
  CCTTTTTTGACTGCTACGAGCCCATGACAATGATGGCAGGGGGTCGTCCTGTGTTTGTGT
  CCCTGAAGCCGGGTCCCATCCAGAATGGAGAACTGGGTTCCAGCAGCAACTGGCAGCTGG
  ACCCCATGGNAGCTGCCGGCANATTCACATCACGCACCANAGCCCTGGTCCTCAACACCC
  CCAACAACCCCCTGGGCAAGGGTGNTCTCCAGGNAAAGAGCTGAGCTGGTGGCCAGCCTT
  TGCCAGCAGCATGACGTGGTGTGTATCACTGATGAAGTCTACCAGTGGATGGTCTACGAC
  GGGCACAGCACATCAGCATTGCCAGCCTNCCTGGCATGTTGGAAACGGACCCTGACCCAT
  CGNAGNNGCNGGCAGAACTTCAGCGCCACTGGGCTGAAAGTGGGGCTGGNTCCCTGGGTC
  CAGAAACATNATGAAGCACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004059 unedited
  NCGGAAATACTATGNTACCGCGGCCGCAATTCTANATCGAGTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTGGTAAGGATCTTAATAACTTAAACATTTTATTATGG
  ACCAAAAGGAAAGGCTTGAGGACTCAAGCCTAAAGGCAACTCCCCAAGGCCAAAATGAAC
  CAGGGAAGGGGAGGTTATACCTGAACCTTAACAGGGGCCATGCATAACTGGGATGTGATC
  GGTACATGGGGGAAGTCTACCGTCCGTCTTAACCAAACCTGGAGAGACCAGAAGCAACAC
  AAGACCCTTCAAACCCACCTATGGAGGGGCAAGGAGAGGCCCAAGTCAAGCCACCCTCAC
  CTTTTAAACAGGAGGCACTTGGTTCTCTAAAAAGAAAAAAGGGGGGTCCCACCCAAAACA
  TTTTGTGTCACGGAAAAAAAGGTTCCCAATAACATCTTCCCCAACCTAAAAATGTCTGAA
  ACCTGGACAAAAACAGACACTCCAAGAGGATCTCTGCGGGCATGTGGGGATGTCAAGGGC
  CAAGCGTGACTTTAGGGGTAGAGTTTCACCTTCCACTTTCGCAACTTTTTGTCCATGGGC
  CGGAACGTGGCTTTATTCTTTACAAAACAAAAACCGATATAGTGGGCAAAATGCTTCTGA
  TGTGGCGCACTATATAAAAAGGGGACACGGGTGGGCACCAAACGCCTGGTTCTTGATCAT
  CCCCTTTACCAAACCTCTGTCATAAGGGTTATTCACAGGTTCCGGGAAGTTAGGGAATTT
  TCTCTTTAAAACTTAAAAGTCTGTGGAGAAGAAAAAACTGCCCCCGAGGAAGAATGGGCT
  TTCAAGCCCACTGCCTGGGGGCTACCTTTCATATTGTCCCCGGAACCCTTCTTGGCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004059
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004059.3, NP_004050.2
• RefSeq Size: 2152 bp
• RefSeq ORF: 753 bp
• Locus ID: 883
• UniProt ID: Q16773
• Cytogenetics: 9q34.11
• Domains: aminotran_1_2
• Gene Summary: This gene encodes a cytosolic enzyme that is responsible for the metabolism of cysteine conjugates of certain halogenated alkenes and alkanes. This metabolism can form reactive metabolites leading to nephrotoxicity and neurotoxicity. Increased levels of this enzyme have been linked to schizophrenia. Multiple transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the isoform a. Variants 1, 2, and 6-11 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.