DCC (NM_005215) Human Untagged Clone

CAT#: SC116848

DCC (untagged)-Human deleted in colorectal carcinoma (DCC)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCC
• Synonyms: CRC18; CRCR1; HGPPS2; IGDCC1; MRMV1; NTN1R1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005215 edited
  GAATTCGGCACGAGGCTCCAGCTCTCGCGCGGAATTGTCTCTTCAACTTTACCCAACCGA
  CGACAAGGAACCAGCCTCAACCTTTTAATGCACAGCCCGGCCACAGGATTGCCTTCCATC
  TCCTCTTGGTCCCTCCTGGATGTGGTTTATTGATGACTTGCGAGCCCCTCAGAGAGCTGT
  CTTCCCTCCTCTGGCTCCCTCCGTTTCCTTGAGTTAGTTTTCTAAGGTTTTACCGGGGCT
  CGGGATCTCTTGGACCGAATGGAACTTTTTGCTGCCTGCTTTTGCTGCTGATTCTGTCAG
  TGGACAAGGAAAAAGGCTTCGAAGGCAGCAGAGGCGCAGGGGAGGTGGAGAAAGAGGTGG
  AGGAAGAGGACGAGGAGGAGGAGGAAGCCGAAGGGGCTCGGCGCGTGTGTGTGCATGTGT
  GCATGCGTGTGTGAGTGCATGTGTGTGAGTGCTGCCGCTGCCCGCGACCCCTGGCCCCGA
  AGGTGTTGGCTGAAATATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTT
  TGTACTCTTCGGAGCTTCCTTGCTCAGCGCGCATCTTCAAGTAACCGGTTTTCAAATTAA
  AGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGG
  AAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAA
  GAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGG
  GTCTCTGCTGATACAAAACATACTTCATTCCAGACACCACAAGCCAGATGAGGGACTTTA
  CCAATGTGAGGCATCTTTAGGAGATTCTGGCTCAATTATTAGTCGGACAGCAAAAGTTGC
  AGTAGCAGGACCACTGAGGTTCCTTTCACAGACAGAATCTGTCACAGCCTTCATGGGAGA
  CACAGTGCTACTCAAGTGTGAAGTCATTGGGGAGCCCATGCCAACAATCCACTGGCAGAA
  GAACCAACAAGACCTGACTCCAATCCCAGGTGACTCCCGAGTGGTGGTCTTGCCCTCTGG
  AGCATTGCAGATCAGCCGACTCCAACCGGGGGACATTGGAATTTACCGATGCTCAGCTCG
  AAATCCAGCCAGCTCAAGAACAGGAAATGAAGCAGAAGTCAGAATTTTATCAGATCCAGG
  ACTGCATAGACAGCTGTATTTTCTGCAAAGACCATCCAATGTAGTAGCCATTGAAGGAAA
  AGATGCTGTCCTGGAATGTTGTGTTTCTGGCTATCCTCCACCAAGTTTTACCTGGTTACG
  AGGCGAGGAAGTCATCCAACTCAGGTCTAAAAAGTATTCTTTATTGGGTGGAAGCAACTT
  GCTTATCTCCAATGTGACAGATGATGACAGTGGAATGTATACCTGTGTTGTCACATATAA
  AAATGAGAATATTAGTGCCTCTGCAGAGCTCACAGTCTTGGTTCCGCCATGGTTTTTAAA
  TCATCCTTCCAACCTGTATGCCTATGAAAGCATGGATATTGAGTTTGAATGTACAGTCTC
  TGGAAAGCCTGTGCCCACTGTGAATTGGATGAAGAATGGAGATGTGGTCATTCCTAGTGA
  TTATTTTCAGATAGTGGGAGGAAGCAACTTACGGATACTTGGGGTGGTGAAGTCAGATGA
  AGGCTTTTATCAATGTGTGGCTGAAAATGAGGCTGGAAATGCCCAGACCAGTGCACAGCT
  CATTGTCCCTAAGCCTGCTATCCCAAGCTCCAGTGTCCTCCCTTCGGCTCCCAGAGATGT
  GGTCCCTGTCTTGGTTTCCAGCCGATTTGTCCGTCTCAGCTGGCGCCCACCTGCAGAAGC
  GAAAGGGAACATTCAAACTTTCACGGTCTTTTTCTCCAGAGAAGGTGACAACAGGGAACG
  AGCATTGAATACAACACAGCCTGGGTCCCTTCAGCTCACTGTGGGAAACCTGAAGCCAGA
  AGCCATGTACACCTTTCGAGTTGTGGCTTACAATGAATGGGGACCGGGAGAGAGTTCTCA
  ACCCATCAAGGTGGCCACACAGCCTGAGTTGCAAGTTCCAGGGCCAGTAGAAAACCTGCA
  AGCTGTATCTACCTCACCTACCTCAATTCTTATTACCTGGGAACCCCCTGCCTATGCAAA
  CGGTCCAGTCCAAGGTTACAGATTGTTCTGCACTGAGGTGTCCACAGGAAAAGAACAGAA
  TATAGAGGTTGATGGACTATCTTATAAACTGGAAGGCCTGAAAAAATTCACCGAATATAG
  TCTTCGATTCTTAGCTTATAATCGCTATGGTCCGGGCGTCTCTACTGATGATATAACAGT
  GGTTACACTTTCTGACGTGCCAAGTGCCCCGCCTCAGAACGTCTCCCTGGAAGTGGTCAA
  TTCAAGAAGTATCAAAGTTAGCTGGCTGCCTCCTCCATCAGGAACACAAAATGGATTTAT
  TACCGGCTATAAAATTCGACACAGAAAGACGACCCGCAGGGGTGAGATGGAAACACTGGA
  GCCAAACAACCTCTGGTACCTATTCACAGGACTGGAGAAAGGAAGTCAGTACAGTTTCCA
  GGTGTCAGCCATGACAGTCAATGGTACTGGACCACCTTCCAACTGGTATACTGCAGAGAC
  TCCAGAGAATGATCTAGATGAATCTCAAGTTCCTGATCAACCAAGCTCTCTTCATGTGAG
  GCCCCAGACTAACTGCATCATCATGAGTTGGACTCCTCCCTTGAACCCAAACATCGTGGT
  GCGAGGTTATATTATCGGTTATGGCGTTGGGAGCCCTTACGCTGAGACAGTGCGTGTGGA
  CAGCAAGCAGCGATATTATTCCATTGAGAGGTTAGAGTCAAGTTCCCATTATGTAATCTC
  CCTAAAAGCTTTTAACAATGCCGGAGAAGGAGTTCCTCTTTATGAAAGTGCCACCACCAG
  GTCTATAACCGATCCCACTGACCCAGTTGATTATTATCCTTTGCTTGATGATTTCCCCAC
  CTCGGTCCCAGATCTCTCCACCCCCATGCTCCCACCAGTAGGTGTACAGGCTGTGGCTCT
  TACCCATGATGCTGTGAGGGTCAGCTGGGCAGACAACTCTGTCCCTAAGAACCAAAAGAC
  GTCTGAGGTGCGACTTTACACCGTCCGGTGGAGAACCAGCTTTTCTGCAAGTGCAAAATA
  CAAGTCAGAAGACACAACATCTCTAAGTTACACAGCAACAGGCCTCAAACCAAACACAAT
  GTATGAATTCTCGGTCATGGTAACAAAAAACAGAAGGTCCAGTACTTGGAGCATGACTGC
  ACATGCCACCACGTATGAAGCAGCCCCCACCTCTGCTCCCAAGGACTTGACAGTCATTAC
  TAGGGAAGGGAAGCCTCGTGCCGTCATTGTGAGTTGGCAGCCTCCCTTGGAAGCCAATGG
  GAAAATTACTGCTTACATCTTATTTTATACCTTGGACAAGAACATCCCAATTGATGACTG
  GATTATGGAAACAATCAGTGGTGATAGGCTTACTCATCAAATCATGGATCTCAACCTTGA
  TACTATGTATTACTTTCGAATTCAAGCACGAAATTCAAAAGGAGTGGGGCCACTCTCTGA
  TCCTATCCTCTTCAGGACTCTGAAAGTGGAACACCCTGACAAAATGGCTAATGACCAAGG
  TCGTCATGGAGATGGAGGTTATTGGCCAGTTGATACTAATTTGATTGATAGAAGCACCCT
  AAATGAGCCGCCAATTGGACAAATGCACCCCCCGCATGGCAGTGTCACTCCTCAGAAGAA
  CAGCAACCTGCTTGTGATCATTGTGGTCACCGTTGGTGTCATCACAGTGCTGGTAGTGGT
  CATCGTGGCTGTGATTTGCACCCGACGCTCTTCAGCCCAGCAGAGAAAGAAACGGGCCAC
  CCACAGTGCTGGCAAAAGGAAGGGCAGCCAGAAGGACCTCCGACCCCCTGATCTTTGGAT
  CCATCATGAAGAAATGGAGATGAAAAATATTGAAAAGCCATCTGGCACTGACCCTGCAGG
  AAGGGACTCTCCCATCCAAAGTTGCCAAGACCTCACACCAGTCAGCCACAGCCAGTCAGA
  AACCCAACTGGGAAGCAAAAGCACCTCTCATTCAGGTCAAGACACTGAGGAAGCAGGGAG
  CTCTATGTCCACTCTGGAGAGGTCGCTGGCTGCACGCCGAGCCCCCCGGGCCAAGCTCAT
  GATTCCCATGGATGCCCAGTCCAACAATCCTGCTGTCGTGAGCGCCATCCCGGTGCCAAC
  GCTAGAAAGTGCCCAGTACCCAGGAATCCTCCCGTCTCCCACCTGTGGATATCCCCACCC
  GCAGTTCACTCTCCGGCCTGTGCCATTCCCAACACTCTCAGTGGACCGAGGTTTCGGAGC
  AGGAAGAAGTCAGTCAGTGAGTGAAGGACCAACTACCCAACAACCACCTATGCTGCCCCC
  ATCTCAGCCTGAGCATTCTAGCAGCGAGGAGGCACCAAGCAGAACCATCCCCACAGCTTG
  TGTTCGACCAACTCACCCACTCCGCAGCTTTGCTAATCCTTTGCTACCTCCACCAATGAG
  TGCAATAGAACCGAAAGTCCCTTACACACCACTTTTGTCTCAGCCAGGGCCCACTCTTCC
  TAAGACCCATGTGAAAACAGCCTCCCTTGGGTTGGCTGGAAAAGCAAGATCCCCTTTGCT
  TCCTGTGTCTGTGCCAACAGCCCCTGAAGTGTCTGAGGAGAGCCACAAACCAACAGAGGA
  TTCAGCCAATGTGTATGAACAGGATGATCTGAGTGAACAAATGGCAAGTTTGGAAGGACT
  CATGAAGCAGCTTAATGCCATCACAGGCTCAGCCTTTTAACATGTATTTCTGAATGGATG
  AGGTGAATTTTCCGGGAACTTTGCAGCATACCAATTACCCATAAACAGCACACCTGTGTC
  CAAGAACTCTAACCAGTGTACAGGTCACCCATCAGGACCACTCAGTTAAGGAAGATCCTG
  AAGCAGTTCAGAAGGAATAAGCATTCCTTCTTTCACAGGCATCAGGAATTGTCAAATGAT
  GATTATGAGTTCCCTAAACAAAAGCAAAGATGCATTTTCACTGCAATGTCAAAGTTTAAG
  CTGCTAGAATAGTCCTGGGCCTTTGTCACTGCAGTGACCACACTGTCATAACTAATACCT
  ATGTTTTCCTTTGTCAAGGCCTGTTGTTTAATGTGTAGGTCTAGTCTTACAAAATGCAAG
  TGCATTATTTAAGCCTGTACCATGCCATGGCAAACCAGTGCAAGCTCACTATTTTGTTTT
  CAACTTAAACATACAAAGCACCCATGGGAATCTCTCATGCCATAGCACCAAAGGATTGGA
  TGTTTTCCTTACAGCACAAAAAGTAAATAGTAAACAAACAAAAGGCAGAGAATGCTTATG
  TTTGTAACTCAGTCATTCATCTTGCACAAGTGGTGGATATTAGTGAGTGGCTAAAAATTC
  ACCTATTTTGGCAAGTATTTGTAAATCCACCCTTGGTTAATATGTATGTCTGGAGTCCAG
  GAATATAAAAATCTGCAACTAGTGGCATTCTGCCAGCAGCAGTACATTTCTGGAAAGAGG
  ATATAATATGCAATCTTCTCAGACACATGGTAATTATATGCTTAAGCTTGTAATAGGACA
  GTTTTCAATTTGGGTGGCTTTTGTGCCATACCACACTGTGATACAATTTCAAAGCTTCAC
  TAAGGCCATCTTCCTTAGGAGTTTGGCCAGAAGAATGCCCCCACCCCTTCACCCCATCCC
  TCCCTGAGTTCTCCTTGGCAACTAGCGTTGGGTGAAATGGCCAGCTCCACATGTCATATG
  GTGCACTGGCCAATGTCGCCTGTCTTCTAATCCCGTAGAAATGGCAXXXXXXXXXXXXXX
  XXXXXXXXXXXXXXXXXTCTGCCTTTGATTTCAAGCTTGAGTAGGTCATAATTTTAAAAG
  AGCATGGAAGGGATAGGATCTTTACAACCTAATAGCTCCTTTTATTAGGTGGGTAATTAT
  ATATGAATCCCTGAATAAAATATTTTGAGCAAAATGGCACTGTAACAGAAGTAATAATTC
  AGTTTATTTTTTTACAGTTTTATGTCGGGAAGGAAATCTGATGTCAAAGAGAGGGCTGTT
  CAAATGGTTCATTAGAAAGTCCGGTCCATTTGCGAATTTGTTCCTTCAACAAGAGTGCTC
  ATTCAAGTTACTCAGATTTTCTGGAAGTCTTTTCTGAAGAGCTATGTGATGTTGTTCTAT
  GGGACAGACTACTCTTATTTAACATCTGGGCACTTAGGTAGACAACCTTCTACTGACCTG
  GAATAAAGTGTTTCCTAACATAATATTGAATTATTCAGAAATAATCCATTACTTCAAAAA
  AGAAAATATTCATTGGGCTAGCCCAACCTTCTCTAGGCCCTAAGAATTATTACCTCCCCT
  TTCTAATTCTAGCAAACATGGAACATTCTCCTTAGGCACTTGACACCCACGAGGGTAATC
  CTGAGTGCTCAGTTTGGAATAGGTTGCAAATCTCAGATTTTAGGGATTGAGTCACACCTT
  CAATCTATAGAATGAAGTTGACCAATTAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005215 unedited
  GTCAGCATTTGTATACGACTCACTATAGGCGGCCGCGNATTCGGCACGAGGCTCCAGCTC
  TCGCGCGGAATTGTCTCTTCAACTTTACCCAACCGACGACAAGGAACCAGCCTCAACCTT
  TTAATGCACAGCCCGGCCACAGGATTGCCTTCCATCTCCTCTTGGTCCCTCCTGGATGTG
  GTTTATTGATGACTTGCGAGCCCCTCAGAGAGCTGTCTTCCCTCCTCTGGCTCCCTCCGT
  TTCCTTGAGTTAGTTTTCTAAGGTTTTACCGGGGCTCGGGATCTCTTGGACCGAATGGAA
  CTTTTTGCTGCCTGCTTTTGCTGCTGATTCTGTCAGTGGACAAGGAAAAAGGCTTCGAAG
  GCAGCAGAGGCGCAGGGGAGGTGGAGAAAGAGGTGGAGGAAGAGGACGAGGAGGAGGAGG
  AAGCCGAAGGGGCTCGGCGCGTGTGTGTGCATGTGTGCATGCGTGTGTGAGTGCATGTGT
  GTGAGTGCTGCCGCTGCCCGCGACCCCTGGCCCCGAAGGTGTTGGCTGAAATATGGAGAA
  TAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCT
  CAGCGCGCATCTTCAAGTAACCGGTTNTCAAATTAAAGCTTTCACAGCACTGCGCTTCCT
  CTCAGAACCTTCTGATGCCGTCACAATGCGGNGAGGAAATGTCCTCCTCGACTGCTCCGC
  GGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGCATTCATCTGGCCTTG
  GGAATGGATGAAAGGAGCAGCAACTTTCAAAAGGGTCTCTGCTGATACAAACATACTTCA
  TTCCAGACACCCAGCCAGATGGAGGACTTTACCATGTGAGCATCTTTAGNAGATCTGGCT
  CAATATTAGTCGACAGCANAGGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005215 unedited
  ATGGCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAATTGGTCAACTTC
  ATTCTATAGATTGAAGGTGTGACTCAATCCCTAAAATCTGAGATTTGCAACCTATTCCAA
  ACTGAGCACTCAGGATTACCCTCGTGGGTGTCAAGTGCCTAAGGAGAATGTTCCATGTTT
  GCTAGAATTAGAAAGGGGAGGTAATAATTCTTAGGGCCTAGAGAAGGTTGGGCTAGCCCA
  ATGAATATTTTCTTTTTTGAAGTAATGGATTATTTCTGAATAATTCAATATTATGTTAGG
  AAACACTTTATTCCAGGTCAGTAGAAGGTTGTCTACCTAAGTGCCCAGATGTTAAATAAG
  AGTAGTCTGTCCCATAGAACAACATCACATAGCTCTTCAGAAAAGACTTCCAGAAAATCT
  GAGTAACTTGAATGAGCACTCTTGTTGAAGGAACAAATTCGCAAATGGACCGGACTTTCT
  AATGAACCATTTGAACAGCCCTCTCTTTGACATCAGATTTCCTTCCCGACATAAAACTGT
  AAAAAAATAAACTGAATTATTACTTCTGTTACAGTGCCATTTTGCTCAAAATATTTTATT
  CAGGGATTCATATATAATTACCCACCTAATAAAAGGAGCTATTAGGTTGTAAAGATCCTA
  TCCCTTTCATGCTCNTTTAAAATTATGACCTACTCCAGCTTGAAATCAAAGGCAGAGCTG
  AAAGGAATAATTCAGCTGAAACTCTCTCATCGGGGTTTCAAGTCACCTAACTGATAAATA
  TGCAAACTAGCTTCCTTTTTGGCGATTCAGCTGCATAACCCAGGATTAACTCCAAATGCA
  GCTGTAAATTCAGAAACTAAANGGCTGCATACCAAATGTTCTTAAATTTTACCTAGTTCA
  AGCTGNAAATAACATGCAATAGAGGTTAAAACGTATCCCAATTTAAGAGCTGCTTGGAAA
  GAATTGCTGGATTACTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005215
• Insert Size: 7800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005215.1, NP_005206.1
• RefSeq Size: 4608 bp
• RefSeq ORF: 4344 bp
• Locus ID: 1630
• UniProt ID: P43146
• Cytogenetics: 18q21.2
• Domains: ig, IGc2, IG, FN3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance, Colorectal cancer, Pathways in cancer
• Gene Summary: This gene encodes a netrin 1 receptor. The transmembrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules, and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand. The cytoplasmic tail interacts with the tyrosine kinases Src and focal adhesion kinase (FAK, also known as PTK2) to mediate axon attraction. The protein partially localizes to lipid rafts, and induces apoptosis in the absence of ligand. The protein functions as a tumor suppressor, and is frequently mutated or downregulated in colorectal cancer and esophageal carcinoma. [provided by RefSeq, Oct 2009]