Glucose Transporter GLUT1 (SLC2A1) (NM_006516) Human Untagged Clone

CAT#: SC116011

SLC2A1 (untagged)-Human solute carrier family 2 (facilitated glucose transporter), member 1 (SLC2A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Glucose Transporter GLUT1
• Synonyms: CSE; DYT9; DYT17; DYT18; EIG12; GLUT; GLUT-1; GLUT1; GLUT1DS; HTLVR; PED; SDCHCN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116011 sequence for NM_006516 edited (data generated by NextGen Sequencing)
  ATGGAGCCCAGCAGCAAGAAGCTGACGGGTCGCCTCATGCTGGCCGTGGGAGGAGCAGTG
  CTTGGCTCCCTGCAGTTTGGCTACAACACTGGAGTCATCAATGCCCCCCAGAAGGTGATC
  GAGGAGTTCTACAACCAGACATGGGTCCACCGCTATGGGGAGAGCATCCTGCCCACCACG
  CTCACCACGCTCTGGTCCCTCTCAGTGGCCATCTTTTCTGTTGGGGGCATGATTGGCTCC
  TTCTCTGTGGGCCTTTTCGTTAACCGCTTTGGCCGGCGGAATTCAATGCTGATGATGAAC
  CTGCTGGCCTTCGTGTCCGCCGTGCTCATGGGCTTCTCGAAACTGGGCAAGTCCTTTGAG
  ATGCTGATCCTGGGCCGCTTCATCATCGGTGTGTACTGCGGCCTGACCACAGGCTTCGTG
  CCCATGTATGTGGGTGAAGTGTCACCCACAGCCCTTCGTGGGGCCCTGGGCACCCTGCAC
  CAGCTGGGCATCGTCGTCGGCATCCTCATCGCCCAGGTGTTCGGCCTGGACTCCATCATG
  GGCAACAAGGACCTGTGGCCCCTGCTGCTGAGCATCATCTTCATCCCGGCCCTGCTGCAG
  TGCATCGTGCTGCCCTTCTGCCCCGAGAGTCCCCGCTTCCTGCTCATCAACCGCAACGAG
  GAGAACCGGGCCAAGAGTGTGCTAAAGAAGCTGCGCGGGACAGCTGACGTGACCCATGAC
  CTGCAGGAGATGAAGGAAGAGAGTCGGCAGATGATGCGGGAGAAGAAGGTCACCATCCTG
  GAGCTGTTCCGCTCCCCCGCCTACCGCCAGCCCATCCTCATCGCTGTGGTGCTGCAGCTG
  TCCCAGCAGCTGTCTGGCATCAACGCTGTCTTCTATTACTCCACGAGCATCTTCGAGAAG
  GCGGGGGTGCAGCAGCCTGTGTATGCCACCATTGGCTCCGGTATCGTCAACACGGCCTTC
  ACTGTCGTGTCGCTGTTTGTGGTGGAGCGAGCAGGCCGGCGGACCCTGCACCTCATAGGC
  CTCGCTGGCATGGCGGGTTGTGCCATACTCATGACCATCGCGCTAGCACTGCTGGAGCAG
  CTACCCTGGATGTCCTATCTGAGCATCGTGGCCATCTTTGGCTTTGTGGCCTTCTTTGAA
  GTGGGTCCTGGCCCCATCCCATGGTTCATCGTGGCTGAACTCTTCAGCCAGGGTCCACGT
  CCAGCTGCCATTGCCGTTGCAGGCTTCTCCAACTGGACCTCAAATTTCATTGTGGGCATG
  TGCTTCCAGTATGTGGAGCAACTGTGTGGTCCCTACGTCTTCATCATCTTCACTGTGCTC
  CTGGTTCTGTTCTTCATCTTCACCTACTTCAAAGTTCCTGAGACTAAAGGCCGGACCTTC
  GATGAGATCGCTTCCGGCTTCCGGCAGGGGGGAGCCAGCCAAAGTGACAAGACACCCGAG
  GAGCTGTTCCATCCCCTGGGGGCTGATTCCCAAGTGTGA
  
  Clone variation with respect to NM_006516.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006516 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAACAGCGAGCGTGCCGGTCG
  CTAGTCGCGGGTCCCCGAGTGAGCACGCCAGGGAGCAGGAGACCAAACGACGGGGGTCGG
  AGTCAGAGTCGCAGTGGGAGTCCCCGGACCGGAGCACGAGCCTGAGCGGGAGAGCGCCGC
  TCGCACGCCCGTCGCCACCCGCGTACCCGGCGCAGCCAGAGCCACCAGCGCAGCGCTGCC
  ATGGAGCCCAGCAGCAAGAAGCTGACGGGTCGCCTCATGCTGGCCGTGGGAGGAGCAGTG
  CTTGGCTCCCTGCAGTTTGGCTACAACACTGGAGTCATCAATGCCCCCCAGAAGGTGATC
  GAGGAGTTCTACAACCAGACATGGGTCCACCGCTATGGGGAGAGCATCCTGCCCACCACG
  CTCACCACGCTCTGGTCCCTCTCAGTGGCCATCTTTTCTGTTGGGGGCATGATTGGCTCC
  TTCTCTGTGGGCCTTTTCGTTAACCGCTTTGGCCGGCGGAATTCAATGCTGATGATGAAC
  CTGCTGGCCTTCGTGTCCGCCGTGCTCATGGGCTTCTCGAAACTGGGCAAGTCCTTTGAG
  ATGCTGATCCTGGGCCGCTTCATCATCGGTGTGTACTGCGGCCTGACCACAGGCTTCGTG
  CCCATGTATGTGGGTGAAGTGTCACCCACAGCCCTTCGTGGGGCCCTGAGCACCCTGCAC
  CAGCTGGGCATCGTCGTCGGCATTCTCATCGCCCCAGTGTTCGGCCTGGACTNCATCATG
  GGGCACAGGNACCTGTGCCCCCTGCTGCTGAGCATCATCTTCATCCCGGGCCTGCTGCAG
  TGCTTCGTGCTGCCTTTCTGCCCCGANAGTCCCCGCTTTCTGCTCATCACCGCACGAGGA
  GACCGGGCCAGTATGTGCTNAAGAGCTGCGCGGNAAAGCTGACGTGACCCATGACTGCAG
  NAATGAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006516 unedited
  GCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTACTATAACTTAGTGTCTGTA
  TTTAATATTGACAACCAAAAATATATATAAATATCTTGCATCTATACACAACAGGGCAGG
  AGTCTCCATGTCTTCTTGAGCAGTGAGTTTGCAGGCTCCCACAGGCCCTCTTCTCATGGT
  AATAGTGTGGCCCTCAGTGCAAAGGAGACTAGAACCCGGCAGCCCAGACTGGCCCTTCCC
  CTCTCCTCCCTGCACTCCAGTGCTCCCAACTGGTCTCAGGTAAAGAAAGATTAATTTGAG
  TGGTTGGGTAGGAAGAGATGGGAAGGGGCAAATCCTAATGGAGCCTGACCCCTAGAGTGG
  GGAGATCCAGGCCAGCAGAACGGGTGGCCATAGCCACCTCCTGGGATAGAAGCTTTGTAG
  TTCATAGTTCGATTAGTGTGTCCTTAGGACATAGGTCCAGCCCTACAGATTAGCTGGGTG
  AAGAAGGCAAGTGTCTCGACAGGGCTTAGTCTCCACCCTCAGGCATGGAACCATTCANGG
  TGAAGCCTGNGATGTGGGCACAGGAGACTCAGGCTGATATAAAATAACAAAATCAGTAAT
  AAAAAAATTATAAAACCTGTTGCTTGTCTGAATAGATTTGAGCAACAGTCTTGCTTTTGN
  TAAAATCCTGGAGCCGTTAAGCTCTGAATATTCCTCCTGACATCATTGCTGGCTGGAGAA
  AGAACNCCCAGCCCGCTCGGCTGACTTCTGTCAGGTTTGGAAATCTCATCCACTGCCCTG
  GCTCCTGAAAGATCCTTAGGCTTCTGGGACCAGCCCGGCTTGTGATCTGGGGCCACTCAC
  CTTGGAAATACCCCCCAGGGAATGAACAGTTCCTCGGTGCCTTGCATTTTGGCTGGTCCC
  CCCGGCGGAACCCGGAGCGATTCCTTGAAGGGCCGCCTCTTAATTCAGAAACTTCTGAAA
  GG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006516
• Insert Size: 2660 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006516.1, NP_006507.1
• RefSeq Size: 2856 bp
• RefSeq ORF: 1479 bp
• Locus ID: 6513
• UniProt ID: P11166
• Cytogenetics: 1p34.2
• Domains: sugar_tr
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Pathways in cancer, Renal cell carcinoma
• Gene Summary: This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013]