AMACR (NM_014324) Human Untagged Clone

CAT#: SC115040

AMACR (untagged)-Human alpha-methylacyl-CoA racemase (AMACR), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AMACR
• Synonyms: AMACRD; CBAS4; P504S; RACE; RM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115040 sequence for NM_014324 edited (data generated by NextGen Sequencing)
  ATGGCACTGCAGGGCATCTCGGTCATGGAGCTGTCCGGCCTGGCCCCGGGCCCGTTCTGT
  GCTATGGTCCTGGCTGACTTCGGGGCGCGTGTGGTACGCGTGGACCGGCCCGGCTCCCGC
  TACGACGTGAGCCGCTTGGGCCGGGGCAAGCGCTCGCTAGTGCTGGACCTGAAGCAGCCG
  CGGGGAGCCGCCGTGCTGCGGCGTCTGTGCAAGCGGTCGGATGTGCTGCTGGAGCCCTTC
  CGCCGCGGTGTCATGGAGAAACTCCAGCTGGGCCCAGAGATTCTGCAGCGGGAAAATCCA
  AGGCTTATTTATGCCAGGCTGAGTGGATTTGGCCAGTCAGGAAGCTTCTGCCGGTTAGCT
  GGCCACGATATCAACTATTTGGCTTTGTCAGGTGTTCTCTCAAAAATTGGCAGAAGTGGT
  GAGAATCCGTATGCCCCGCTGAATCTCCTGGCTGACTTTGCTGGTGGTGGCCTTATGTGT
  GCACTGGGCATTATAATGGCTCTTTTTGACCGCACACGCACTGACAAGGGTCAGGTCATT
  GATGCAAATATGGTGGAAGGAACAGCATATTTAAGTTCTTTTCTGTGGAAAACTCAGAAA
  TCGAGTCTGTGGGAAGCACCTCGAGGACAGAACATGTTGGATGGTGGAGCACCTTTCTAT
  ACGACTTACAGGACAGCAGATGGGGAATTCATGGCTGTTGGAGCAATAGAACCCCAGTTC
  TACGAGCTGCTGATCAAAGGACTTGGACTAAAGTCTGATGAACTTCCCAATCAGATGAGC
  ATGGATGATTGGCCAGAAATGAAGAAGAAGTTTGCAGATGTATTTGCAAAGAAGACGAAG
  GCAGAGTGGTGTCAAATCTTTGACGGCACAGATGCCTGTGTGACTCCGGTTCTGACTTTT
  GAGGAGGTTGTTCATCATGATCACAACAAGGAACGGGGCTCGTTTATCACCAGTGAGGAG
  CAGGACGTGAGCCCCCGCCCTGCACCTCTGCTGTTAAACACCCCAGCCATCCCTTCTTTC
  AAAAGGGATCCTTTCATAGGAGAACACACTGAGGAGATACTTGAAGAATTTGGATTCAGC
  CGCGAAGAGATTTATCAGCTTAACTCAGATAAAATCATTGAAAGTAATAAGGTAAAAGCT
  AGTCTCTAA
  
  Clone variation with respect to NM_014324.5
  25 g=>a;524 g=>a;602 t=>c;829 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014324 unedited
  TAGATTTTGTAACACGATTTACTATAGGGCGGCCGCGAATTCGCACGAGGCTGGGGCTAA
  GGGCTGCTCAGTTTCCTTCAGCGGGGCACTGGGAAGCGCCATGGCACTGCAGGGCATCTC
  GGTCATGGAGCTGTCCGGCCTGGCCCCGGGCCCGTTCTGTGCTATGGTCCTGGCTGACTT
  CGGGGCGCGTGTGGTACGCGTGGACCGGCCCGGCTCCCGCTACGACGTGAGCCGCTTGGG
  CCGGGGCAAGCGCTCGCTAGTGCTGGACCTGAAGCAGCCGCGGGGAGCCGCCGTGCTGCG
  GCGTCTGTGCAAGCGGTCGGATGTGCTGCTGGAGCCCTTCCGCCGCGGTGTCATGGAGAA
  ACTCCAGCTGGGCCCAGAGATTCTGCAGCGGGAAAATCCAAGGCTTATTTATGCCAGGCT
  GAGTGGATTTGGCCAGTCAGGAAGCTTCTGCCGGTTAGCTGGCCACGATATCAACTATTT
  GGCTTTGTCAGGTGTTCTCTCAAAAATTGGCAGAAGTGGTGAGAATCCGTATGCCCCGCT
  GAATCTCCTGGCTGACTTTGCTGGTGGTGGCCTTATGTGTGCACTGGGCATTATAATGGC
  TCTTTTTGACCGCACACGCACTGACAAGGGTCAGGTCATTGATGCAAATATGGTGGAAGG
  AACAGCATATTTAAGTTCTTTTCTGTGGAAAACTCAGAAATCGAGTCTGTGGGAAGCACC
  TCGAGGACAGAACATGTNGNATGGTGGAGCACCTTTCTATACGACTTTACAGACAGCAGA
  TGGGGAATTCATGGCTGNTGGAGCAATAGAACCCAGNTCTACGAGCTGCTGATCAAAGGA
  CTTGNACTAAAGTCTGATGAACTCCCCATCAAAGAGCATGGATGATTNGCCAAAAATGAG
  AAGAGTTTGCAGAGTATTTGCAAAAAGACGAAGCAGAGTGTGTCAAATCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_014324 unedited
  GCCGCATTCTAGTATCGAGTTTTTTTTTTTTTTTTTTAGGGAAAACATCTTTTTTATTAT
  TATTATTTTTATTTATTTATTTAAAGACAGGTTCTTGTTCTGTTGCCTGGGCTAGAGTGC
  AGTGGTGCAATCCTAATTCACTGTGGCCTCAAATTCCTGGGCTCAAGTGATCCTCCTGCC
  CCACCCTTGAGTAGATAGGACTACAGGCACACACCACCATACCTGGATAATTTTTTTATT
  TTTATTTCGTAGAAACGGAGGTCCAGCCAAGTTCCCCAGGCTGGTCTCGAACTCTTGGCC
  TCAAGTGATCCTCCATGCCTGGGCTGGAAAACATAACCTTATGGGAAAGTTCCTAAAAAT
  GGTTGAAGAACAAGACTGAAGGCTAGAATATGAAAGCTTAAATTCTGATTCTGCCCCTTC
  CTAGTTGCATATTCCTGAGCAAATATTTTCAACTGGTATTTCTAAAACTGTGCCAAGGCA
  CCCAGGGCATTAGAGTGACCTCACAAGAGTATCTCAGAATATTTAAAAATTTGAACACAC
  AAACACAATGAAACTCAACATCTGTCAGACATCACGTGAACTACTAGTGAGGTAGTTCAT
  TCTTTCAACATAAGATTGCACTATAATCCTTCTGATAAATTCATATTTTCGTGAGACTGG
  GTTTTCCAGGCCATCTGATAAAAAATCAGTACTATATGGACACCAACATGGAGCATAAAA
  TGAGGGCAGCATTGTCCAATCTCATTTCAAGTGAGGAAGTTGCACAGTGTCCAATAGGTG
  CATACCTCTTCTTAGTAAGTAGTTGGGGATTACCATGAAATAAATTGAAAAATTATTTTT
  TATTAAAGGGCATAATATTCTTCAGCAGCCTAAAAGTGGGAAGGACATATTTTAATTCCG
  TGGGGACTCATTATTTATAATAGAATGGTAAATATTTCTTTGGCCTACCACCCTGAAAAT
  ACCGTGGCCCAAGGGAACTGACCAAAAACCCTTGATCTCTGTTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014324
• Insert Size: 3410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014324.4, NP_055139.4
• RefSeq Size: 2534 bp
• RefSeq ORF: 1149 bp
• Locus ID: 23600
• UniProt ID: Q9UHK6
• Cytogenetics: 5p13.2
• Domains: CAIB-BAIF
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Primary bile acid biosynthesis
• Gene Summary: This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1, also referred to as AMACR IA.