EGLN2 (NM_080732) Human Untagged Clone

CAT#: SC110740

EGLN2 (untagged)-Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EGLN2
• Synonyms: EIT-6; EIT6; HIF-PH1; HIFPH1; HPH-1; HPH-3; PHD1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_080732, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACAGCCCGTGCCAGCCGCAGCCCCTAAGTCAGGCTCTCCCTCAGTTACCAGGGTCTTCGTCAGAGC
  CCTTGGAGCCTGAGCCTGGCCGGGCCAGGATGGGAGTGGAGAGTTACCTGCCCTGTCCCCTGCTCCCCTC
  CTACCACTGTCCAGGAGTGCCTAGTGAGGCCTCGGCAGGGAGTGGGACCCCCAGAGCCACAGCCACCTCT
  ACCACTGCCAGCCCTCTTCGGGACGGTTTTGGCGGGCAGGATGGTGGTGAGCTGCGGCCGCTGCAGAGTG
  AAGGCGCTGCAGCGCTGGTCACCAAGGGGTGCCAGCGATTGGCAGCCCAGGGCGCACGGCCTGAGGCCCC
  CAAACGGAAATGGGCCGAGGATGGTGGGGATGCCCCTTCACCCAGCAAACGGCCCTGGGCCAGGCAAGAG
  AACCAGGAGGCAGAGCGGGAGGGTGGCATGAGCTGCAGCTGCAGCAGTGGCAGTGGTGAGGCCAGTGCTG
  GGCTGATGGAGGAGGCGCTGCCCTCTGCGCCCGAGCGCCTGGCCCTGGACTATATCGTGCCCTGCATGCG
  GTACTACGGCATCTGCGTCAAGGACAGCTTCCTGGGGGCAGCACTGGGCGGTCGCGTGCTGGCCGAGGTG
  GAGGCCCTCAAACGGGGTGGGCGCCTGCGAGACGGGCAGCTAGTGAGCCAGAGGGCGATCCCGCCGCGCA
  GCATCCGTGGGGACCAGATTGCCTGGGTGGAAGGCCATGAACCAGGCTGTCGAAGCATTGGTGCCCTCAT
  GGCCCATGTGGACGCCGTCATCCGCCACTGCGCAGGGCGGCTGGGCAGCTATGTCATCAACGGGCGCACC
  AAGGCCATGGTGGCGTGTTACCCAGGCAACGGGCTCGGGTACGTAAGGCACGTTGACAATCCCCACGGCG
  ATGGGCGCTGCATCACCTGTATCTATTACCTGAATCAGAACTGGGACGTTAAGGTGCATGGCGGCCTGCT
  GCAGATCTTCCCTGAGGGCCGGCCCGTGGTAGCCAACATCGAGCCACTCTTTGACCGGTTGCTCATTTTC
  TGGTCTGACCGGCGGAACCCCCACGAGGTGAAGCCAGCCTATGCCACCAGGTACGCCATCACTGTCTGGT
  ATTTTGATGCCAAGGAGCGGGCAGCAGCCAAAGACAAGTATCAGCTAGCATCAGGACAGAAAGGTGTCCA
  AGTACCTGTATCACAGCCGCCTACGCCCACCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_080732 unedited
  TAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGGCCGGAG
  GAAAAACTCGCCACCCTGAAGGTCCCTTCCCAAGCCCTTAGGGACCGCAGAGGACTTGGG
  GACCAGCAAGCAACCCCCAGGGCACGAGAAGAGCTCTTGCTGTCTGCCCTGCCTCACCCT
  GCCCCACGCCAGGCCCGGTGGCCCCCAGCTGCATCAAGTGGAGGCGGAGGAGGAGGCGGA
  GGAGGGTGGCACCATGGGCCCGGGCGGTGCCCTCCATGCCCGGGGGATGAAGACACTGCT
  GCCATGGACAGCCCGTGCCAGCCGCAGCCCCTAAGTCAGGCTCTCCCTCAGTTACCAGGG
  TCTTCGTCAGAGCCCTTGGAGCCTGAGCCTGGCCGGGCCAGGATGGGAGTGGAGAGTTAC
  CTGCCCTGTCCCCTGCTCCCCTCCTACCACTGTCCAGGAGTGCCTAGTGAGGCCTCGGCA
  GGGAGTGGGACCCCCAGAGCCACAGCCACCTCTACCACTGCCAGCCCTCTTCGGGACGGT
  TTTGGCGGGCAGGATGGTGGTGAGCTGCGGCCGCTGCAGAGTGAAGGCGCTGCAGCGCTG
  GTCACCAAGGGGTGCCAGCGATTGGCAGCCCAGGGCGCACGGGCCTGAGCCCCCAAACGG
  AAATGGGCCGANGATGGTGGGGATGCCCCTTCACCAGCAAACGGCCCTGGGCCAGGCAAG
  AGAACCANGANGCANAGCGGGAGGGTGGCATGAGCTGCAGCTGCAGCAGTGGCAGTGGTG
  AAGCCAGNNTGCTGGCTGATGGAGAAGCGCTGNCCTCTGCGCCCGAGCGCCTGCCCTGGA
  CTATATCGTGCCCTGCATGCNGTACTACNGCATCTGCGTCAAGGACAGCTTNCTGGGGGC
  ANCACTGGNCNGTTCNCGTGCTTGGCCGAGGTGNAGGCCCTCAAAACGGGTGTGCCGCCT
  GCNAAACCGGCANCTAGTGAGGCANATGGCGATCCNGNCGNGCAGCATCCGTGGGGACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_080732 unedited
  TGAACCGCCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAGAGTGTGCAGC
  TCTGAGGTAAACTTTTATTTCTTGGCTGACAGGACTTCCACACCCATGCTCCTCCTGGCA
  CCTTTTACTCTGCCCAAGCAGTGGCAGCCCAGTCCAAAAGGTGCCTGCCATGGCCAAAGG
  GGTTGGGGAGTCACTTCACATTCCAGGCTGGAGGAGTGAGAGGACTTGGGGGTACTCCTC
  CCACCCCATTGCCAGCAAGAGGCCATGACTCACCTCAACCCCAAGTCCTGCATCCTGGTG
  GGAAGTGACAATGCCTCCTCCCAGCTCTCCATACCTAATGGGGTCATGACCAGACAGGGT
  AGGACCCAGGCCCCCAGCTTCAGTTGGTAACGGTGGCCTCCACGGCTGCCCCCTGTCCAG
  GTGACAACCCCAGCCCCCATGATGGGGCAGCAAAGGTCTCTTCTCCTCCTTGGTCCTCAG
  CAACAGACAGAGCCCTCCACACCAGGCAGGACAGAGGCAAAGTCAGAAGCAGCAGCGGTG
  GCAGGGAAGGAGCAGCCAGCACAGGCCCAGGGCTCTCCTGAAGTCATTTAAGCTGTCTGC
  CATGCGGCTCTGGGACTGGCCACTAGGTGGGCGTAGGCGGCTGTGATACAGGTACTTGGA
  CACCTTTCTGTCCTGATGCTAGCTGATACTTGTCTTTGGCTGCTGCCCGCTCCTTGGCAT
  CAAAATACCAGACAGTGATGGCGTACCTGGTGGCATANGCTGGCTTCACCTCGTGGGGGT
  TCCGCCGGTCAGACCCAAAATGAGCAACCCGTCAAAGATGGCTCGATGTGGCTACACGGG
  CCGGCCCTCAGGGAAGATCTGAGCAGGCCGCCTGCACCTTACGTCCCATTTCTGATCAGG
  TATAAATACGGTGATGCAACCCCCATTCCCTGGGGATTGTAACGTGCCTTAGTACCCAAG
  CCGTTGCCTGGTTAACACCACCAGGCCTGGTGCCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_080732
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080732.1, NP_542770.1
• RefSeq Size: 2174 bp
• RefSeq ORF: 378 bp
• Locus ID: 112398
• UniProt ID: Q96KS0
• Cytogenetics: 19q13.2
• Domains: 2OG-FeII_Oxy, P4Hc
• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, Renal cell carcinoma
• Gene Summary: The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011]
  Transcript Variant: This variant (3) represents the longer transcript. Both variants 1 and 3 utilize alternative start codons that result in expression of two isoforms known as PHD1p43 and PHD1p40.