CCN4 (NM_080838) Human Untagged Clone

CAT#: SC109994

WISP1 (untagged)-Human WNT1 inducible signaling pathway protein 1 (WISP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCN4
• Synonyms: WISP1; WISP1-OT1; WISP1-UT1; WISP1c; WISP1i; WISP1tc
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109994 sequence for NM_080838 edited (data generated by NextGen Sequencing)
  ATGAGGTGGTTCCTGCCCTGGACGCTGGCAGCAGTGACAGCAGCAGCCGCCAGCACCGTC
  CTGGCCACGGCCCTCTCTCCAGCCCCTACGACCATGGACTTTACCCCAGCTCCACTGGAG
  GACACCTCCTCACGCCCCCAATTCTGCAAGTGGCCATGTGAGTGCCCGCCATCCCCACCC
  CGCTGCCCGCTGGGGGTCAGCCTCATCACAGATGGCTGTGAGTGCTGTAAGATGTGCGCT
  CAGCAGCTTGGGGACAACTGCACGGAGGCTGCCATCTGTGACCCCCACCGGGGCCTCTAC
  TGTGACTACAGCGGGGACCGCCCGAGGTACGCAATAGGAGTGTGTGCACATGCTGTGGGT
  GAGGTGGAGGCATGGCACAGGAACTGCATAGCCTACACAAGCCCCTGGAGCCCTTGCTCC
  ACCAGCTGCGGCCTGGGGGTCTCCACTCGGATCTCCAATGTTAACGCCCAGTGCTGGCCT
  GAGCAAGAGAGCCGCCTCTGCAACTTGCGGCCATGCGATGTGGACATCCATACACTCATT
  AAGGCAGGGAAGAAGTGTCTGGCTGTGTACCAGCCAGAGGCATCCATGAACTTCACACTT
  GCGGGCTGCATCAGCACACGCTCCTATCAACCCAAGTACTGTGGAGTTTGCATGGACAAC
  AGGTGCTGCATCCCCTACAAGTCTAAGACTATCGACGTGTCCTTCCAGTGTCCTGATGGG
  CTTGGCTTCTCCCGCCAGGTCCTATGGATTAATGCCTGCTTCTGTAACCTGAGCTGTAGG
  AATCCCAATGACATCTTTGCTGACTTGGAATCCTACCCTGACTTCTCAGAAATTGCCAAC
  TAG
  
  Clone variation with respect to NM_080838.2
  660 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_080838 unedited
  ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGGCCAGTCT
  GGGCCCAGCTCCCCCGAGAGTGGTCGGATCCTCTGGGCTGCTCGGTCGATGCCTGTGCCA
  CTGACGTCCAGGCATGAGGTGGTTCCTGCCCTGGACGCTGGCAGCAGTGACAGCAGCAGC
  CGCCAGCACCGTCCTGGCCACGGCCCTCTCTCCAGCCCCTACGACCATGGACTTTACCCC
  AGCTCCACTGGAGGACACCTCCTCACGCCCCCAATTCTGCAAGTGGCCATGTGAGTGCCC
  GCCATCCCCACCCCGCTGCCCGCTGGGGGTCAGCCTCATCACAGATGGCTGTGAGTGCTG
  TAAGATGTGCGCTCAGCAGCTTGGGGACAACTGCACGGAGGCTGCCATCTGTGACCCCCA
  CCGGGGCCTCTACTGTGACTACAGCGGGGACCGCCCGAGGTACGCAATAGGAGTGTGTGC
  ACATGCTGTGGGTGAGGTGGAGGCATGGCACAGGAACTGCATAGCCTACACAAGCCCCTG
  GAGCCCTTGCTCCACCAGCTGCGGCCTGGGGGTCTCCACTCGGATCTCCAATGTTAACGC
  CCAGTGCTGGCCTGAGCAAGAGAGCCGCCTCTGCAACTTGCGGCCATGCGATGTGGACAT
  CCATACACTCATTAAGGCAGGGAAGAAGTGTCTGGCTGTGTACCAGCCAGAGGCATCCAT
  GAAACTCACACTTGCGGGCTGCATCAGCACACGCTCCTATCAACCCAGTACTGTGGAGTT
  TGCATGGACCACAAGTGCTGCATCCCCTACAAGTCTAAGACTATCGACGTGTCCTTCCAA
  TGGTCTGATGGGCGTGGCTTCTCCCGCCAAGCCTATGGATTATGACTGCTTCTGTACCTG
  AGCTGTAGGAATCCAAGACATCTTGCTGACTTGGAATCTACCTGACTCTCCGAAATGCAA
  CTAAGCAGCCAAACTTGGTCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_080838 unedited
  ATGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGACATTAAAAAATA
  AGCCATTTATTGCAATGTTTTCCTATTCTGACACATTTGGAAGCTCTAAAAACAGGGGGA
  AAATATGGGTTATAAAGACAGTCCAATTTCGATCTGAATCCTAACTTTCCCAGTCACCAA
  CTGGGTGACTCTGGTTGAGTTTTCTCACCTCTATGAGCTTCCGTTTCACATTTGTAAGAA
  CAGAAATAATAATGGGAACTTCTCAACATTGTCGTGAAGATTCAGTGAGCTAATACATGC
  AAAGCACTGAGAGCAATGTCTGGCACAGAAAAGGCCCTAAATAAAAGAATTCTAACAGTA
  AATATTATATTTTCCTAATACAATAAATAATAGCCTGATATTCATTAACAACAACCTTTT
  CCTTTTAGCTTCAACCTCTTCAGCTTTAAACCTTTATTAAGTCTATTTCTGTTTCTAAAC
  AACCGGTAAACCTCCATCTTCCTACCAGAAAACTGGCCATTTGGTCAGCAGAACGAAAGG
  CTGTGAAATGAGTCTCTGCAATAGATGTGTCCTGAACACGGGGGCCCCTGGAGTCCCACA
  GCCTCCAGCTCTAGAGGTCACCCCAGGCTGCTACCAAGGGCACCCACTTCTTGGTTTGAC
  CCACACCCCTACCTGAACCTGTCTTGGTCATTTCAGTAGGTCTGGCATGGTCTGCAGCTG
  GGTCTCTCAAGGCTCTGTAAGAGTAGAGGAGTGTTCCAGGGCACCACTCTCAGGGGCCAG
  ACAGGCCAGTCCCAGAGCCTTACCAATTCCTATGACCCCCAAGCAGACAAGGGAGGAGAT
  TCAAGAAAGAGCCTGGGCAACTTGCCAAAGCCCTGCCAGTCAACAGCTGATTCAGTGTGA
  GAGCCAATGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_080838
• Insert Size: 2660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080838.1, NP_543028.1
• RefSeq Size: 1035 bp
• RefSeq ORF: 843 bp
• Locus ID: 8840
• UniProt ID: O95388
• Cytogenetics: 8q24.22
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - DSL/Notch pathway, Stem cell relevant signaling - Wnt Signaling pathway
• Gene Summary: This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2011]
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, as compared to variant 1. Isoform 2 encoded by this variant thus lacks a von Willbrand type C module that is thought to participate in protein complex formation. This variant is overexpressed in scirrhous carcinomas. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.