GABA A Receptor beta 3 (GABRB3) (NM_021912) Human Untagged Clone

CAT#: SC109260

GABRB3 (untagged)-Human gamma-aminobutyric acid (GABA) A receptor, beta 3 (GABRB3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GABA A Receptor beta 3
• Synonyms: DEE43; ECA5; EIEE43
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021912, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGCTCCGGGCTCCTGGAGCTCCTGCTGCCCATCTGGCTCTCCTGGACCCTGGGGACCCGAGGCTCTG
  AGCCCCGCAGTGTGAACGATCCCGGGAACATGTCCTTTGTGAAGGAGACGGTGGACAAGCTGTTGAAAGG
  CTACGACATTCGCCTAAGACCCGACTTCGGGGGTCCCCCGGTCTGCGTGGGGATGAACATCGACATCGCC
  AGCATCGACATGGTTTCCGAAGTCAACATGGATTATACCTTAACCATGTATTTTCAACAATATTGGAGAG
  ATAAAAGGCTCGCCTATTCTGGGATCCCTCTCAACCTCACGCTTGACAATCGAGTGGCTGACCAGCTATG
  GGTGCCCGACACATATTTCTTAAATGACAAAAAGTCATTTGTGCATGGAGTGACAGTGAAAAACCGCATG
  ATCCGTCTTCACCCTGATGGGACAGTGCTGTATGGGCTCAGAATCACCACGACAGCAGCATGCATGATGG
  ACCTCAGGAGATACCCCCTGGACGAGCAGAACTGCACTCTGGAAATTGAAAGCTATGGCTACACCACGGA
  TGACATTGAGTTTTACTGGCGAGGCGGGGACAAGGCTGTTACCGGAGTGGAAAGGATTGAGCTCCCGCAG
  TTCTCCATCGTGGAGCACCGTCTGGTCTCGAGGAATGTTGTCTTCGCCACAGGTGCCTATCCTCGACTGT
  CACTGAGCTTTCGGTTGAAGAGGAACATTGGATACTTCATTCTTCAGACTTATATGCCCTCTATACTGAT
  AACGATTCTGTCGTGGGTGTCCTTCTGGATCAATTATGATGCATCTGCTGCTAGAGTTGCCCTCGGGATC
  ACAACTGTGCTGACAATGACAACCATCAACACCCACCTTCGGGAGACCTTGCCCAAAATCCCCTATGTCA
  AAGCCATTGACATGTACCTTATGGGCTGCTTCGTCTTTGTGTTCCTGGCCCTTCTGGAGTATGCCTTTGT
  CAACTACATTTTCTTTGGAAGAGGCCCTCAAAGGCAGAAGAAGCTTGCAGAAAAGACAGCCAAGGCAAAG
  AATGACCGTTCAAAGAGCGAAAGCAACCGGGTGGATGCTCATGGAAATATTCTGTTGACATCGCTGGAAG
  TTCACAATGAAATGAATGAGGTCTCAGGCGGCATTGGCGATACCAGGAATTCAGCAATATCCTTTGACAA
  CTCAGGAATCCAGTACAGGAAACAGAGCATGCCTCGAGAAGGGCATGGGCGATTCCTGGGGGACAGAAGC
  CTCCCGCACAAGAAGACCCATCTACGGAGGAGGTCTTCACAGCTCAAAATTAAAATACCTGATCTAACCG
  ATGTGAATGCCATAGACAGATGGTCCAGGATCGTGTTTCCATTCACTTTTTCTCTTTTCAACTTAGTTTA
  CTGGCTGTACTATGTTAACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021912 unedited
  NGGGGTGCAAATTTGTATACGACTCCTATAGGCGGCNCGCGAATTCGCACGAGGCTGCTC
  GGAGAGTAGGGGGGAGAGCGGATCCCAGCAGGTTAGGCCGGAGGAACAGCGCCATGTGCT
  CCGGGCTCCTGGAGCTCCTGCTGCCCATCTGGCTCTCCTGGACCCTGGGGACTCGAGGCT
  CTGAGCCTCGCAGTGTGAACGATCCCGGGAACATGTCCTTTGTGAAGGAGACGGTGGACA
  AGCTGTTGAAAGGCTACGACATTCGCCTAAGACCCGACTTCGGGGGTCCCCCGGTCTGCG
  TGGGGATGAACATCGACATCGCCAGCATCGACATGGTTTCCGAAGTCAACATGGATTATA
  CCTTAACCATGTATTTTCAACAATATTGGAGAGATAAAAGGCTCGCCTATTCTGGGATCC
  CTCTCAACCTCACGCTTGACAATCGAGTGGCTGACCAGCTATGGGTGCCCGACACATATT
  TCTTAAATGACAAAAAGTCATTTGTGCATGGAGTGACAGTGAAAAACCGCATGATCCGTC
  TTCACCCTGATGGGACAGTGCTGTATGGGCTCAGAATCACCACGACAGCAGCATGCATGA
  TGGACCTCAGGAGATACCCCCTGGACGAGCAGAACTGCACTCTGGAAATTGAAAGCTATG
  GCTACACCACGGATGACATTGAGTTTTACTGGCGAGGCGGNGACAAGGCTGTTACCGGAG
  TGGAAAGGATTGAGCTCCCGCAGTTCTCCATCGTGGAGCACCGTCTGGTCTCGAGGAATG
  TTGTCTTCGCCACAGGTGCCTATCCTCGACTGTCACTGAGCTNTCGGTTGAAGAGGACAT
  TGGATACTTCATTCTTCAGACTATATGCCCTCTATCTGATACGATNCTGTCGNGGGTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021912 unedited
  AGCTATGNACCGCGGCACGCATNCTAGTGTCGAGTTTTTTTTTTTTTTTTTTCTTTAAAA
  AGACTCATCTAAATAGTAAGTTAAGGTTGCGTCTATGAAACCGGTGCACCTTGTCTTGTT
  TCTACGAGCTTTAAAAAGTCCAAATACTGATGAGGCTGTGGATCAGAAGGAACCAACAGT
  GACTGTGTTTGCTGTCAGGTTGCCCTCCTTTGCCTCAAAGAACGGTCATGGGTCGTTGGG
  TGACGCGTGGTGTGTACGGGTCATGTTCCTGCAGAATCCTATTCAGTCTAGGAGTAGTTG
  GATGTGCTTATGAAATATGTCAGATACAGAAATAAAGGCATGAGGATGATTCTTAGTTTC
  TGAAATTGAACAGTAGAAGTGTTTTAGCTTCAATCTTAATTTCTAGATAACAGAAAGTAG
  TTACATCACATAAAGGAAAATATGACATCGCATGCTGCCATGATAGCAAGCGTAAGAAAC
  AAAATCTTGGTAAATATACATATATATGCATATTATGCAAATATATTTTCTATTAACATA
  CAATGTTATAAGAATGATAACATAACTGCAAGGGGGAAAATCATCCTAGATGGTTTTACC
  TTAGAGTAACGAATAACCTGAGACGTCTATGCTTTCTGTTGGATATCAGGCCTAGAAATC
  CATACGAGATGTAGAGCACATTTTCAGTGATTGAAATGTCACTGGCCAAACGTGTCACTG
  TGTTCGCACATGACAGTGAAGCTGGATCCACTCCAAGTGAACAACAGATACAAACTACAC
  ACTGTATTTTAGAGTACTTACAACGAGATGCCATTCACTTCCGATGAGTTTCAAAAGACG
  TCGTTCAGACTCAACGTGGGATCTATCTATGTTCAGACACAGGTAAGCCACTGCCAGTGG
  CTCACCCAGCCACTAACCATTGCAGAAACGTTGCCCGATGAAACATACCCTCACCGAGAA
  GCCAGAAGCTTTGCTACTAACTGACGANG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021912
• Insert Size: 3090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021912.2, NP_068712.1
• RefSeq Size: 2970 bp
• RefSeq ORF: 1422 bp
• Locus ID: 2562
• UniProt ID: P28472
• Cytogenetics: 15q12
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a member of the ligand-gated ionic channel family. The encoded protein is one the subunits of a multi-subunit chloride channel that serves as the receptor for gamma-aminobutyric acid, a major inhibitory neurotransmitter of the mammalian nervous system. This gene is located on the long arm of chromosome 15 in a cluster with two other genes encoding related subunits of the family. This gene may be associated with the pathogenesis of several disorders including Angelman syndrome, Prader-Willi syndrome, nonsyndromic orofacial clefts, epilepsy and autism. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (2) uses an alternate 5' terminal exon compared to variant 1. It encodes isoform 2, which is of the same length, but has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.