CD97 (ADGRE5) (NM_001784) Human Untagged Clone

CAT#: SC109040

CD97 (untagged)-Human CD97 molecule (CD97), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD97
• Synonyms: CD97; TM7LN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109040 sequence for NM_001784 edited (data generated by NextGen Sequencing)
  ATGGGAGGCCGCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGAAACC
  CAGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGAACTCCTCGTGTGTCAATGCCACC
  GCCTGTCGCTGCAATCCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCGACGGAG
  ACTTGTGACGACATCAACGAGTGTGCAACACCGTCGAAAGTGTCATGCGGAAAATTCTCG
  GACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCGGGATATGAGCCTGTT
  TCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAACACCTGTCAAGATGTGGACGAGTGC
  AGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGGGTTCATAC
  AGCTGCCGCTGCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGAATAACCAAAAGGAC
  ACTGTCTGTGAAGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAG
  ACGCTTTCCCGATTCTTCGACAAAGTCCAGGACCTGGGCAGAGACTCCAAGACAAGCTCA
  GCCGAGGTCACCATCCAGAATGTCATCAAATTGGTGGATGAACTGATGGAAGCTCCTGGA
  GACGTAGAGGCCCTGGCGCCACCTGTCCGGCACCTCATAGCCACCCAGCTGCTCTCAAAC
  CTTGAAGATATCATGAGGATCCTGGCCAAGAGCCTGCCTAAAGGCCCCTTCACCTACATT
  TCCCCTTCGAACACAGAGCTGACCCTGATGATCCAGGAGCGGGGGGACAAGAACGTCACT
  ATGGGTCAGAGCAGCGCACGCATGAAGCTGAATTGGGCTGTGGCAGCTGGAGCCGAGGAT
  CCAGGCCCCGCCGTGGCGGGCATCCTCTCCATCCAGAACATGACGACATTGCTGGCCAAT
  GCCTCCTTGAACCTGCATTCCAAGAAGCAAGCCGAACTGGAGGAGATATATGAAAGCAGC
  ATCCGTGGTGTCCAACTCAGACGCCTCTCTGCCGTCAACTCCATCTTTCTGAGCCACAAC
  AACACCAAGGAACTCAACTCCCCCATCCTTTTCGCCTTCTCCCACCTTGAGTCCTCCGAT
  GGGGAGGCGGGAAGAGACCCTCCTGCCAAGGACGTGATGCCTGGGCCACGGCAGGAGCTG
  CTCTGTGCCTTCTGGAAGAGTGACAGCGACAGGGGAGGGCACTGGGCCACCGAGGGCTGC
  CAGGTGCTGGGCAGCAAGAACGGCAGCACCACCTGCCAATGCAGCCACCTGAGCAGCTTT
  GCGATCCTTATGGCTCATTATGACGTGGAGGACTGGAAGCTGACCCTGATCACCAGGGTG
  GGACTGGCGCTGTCACTCTTCTGCCTGCTGCTGTGCATCCTCACTTTCCTGCTGGTGCGG
  CCCATCCAGGGCTCGCGCACCACCATACACCTGCACCTCTGCATCTGCCTCTTCGTGGGC
  TCCACCATCTTCCTGGCCGGCATCGAGAACGAAGGCGGCCAGGTGGGGCTGCGCTGCCGC
  CTGGTGGCCGGGCTGCTGCACTACTGTTTCCTGGCCGCCTTCTGCTGGATGAGCCTCGAA
  GGCCTGGAGCTCTACTTTCTTGTGGTGCGCGTGTTCCAAGGCCAGGGCCTGAGTACGCGC
  TGGCTCTGCCTGATCGGCTATGGCGTGCCCCTGCTCATCGTGGGCGTCTCGGCTGCCATC
  TACAGCAAGGGCTACGGCCGCCCCAGATACTGCTGGTTGGACTTTGAGCAGGGCTTCCTC
  TGGAGCTTCTTGGGACCTGTGACCTTCATCATTTTGTGCAATGCTGTCATTTTCGTGACT
  ACCGTCTGGAAGCTCACTCAGAAGTTTTCTGAAATCAATCCAGACATGAAGAAATTAAAG
  AAGGCGAGGGCGCTGACCATCACGGCCATCGCGCAGCTCTTCCTGTTGGGCTGCACCTGG
  GTCTTTGGCCTGTTCATCTTCGACGATCGGAGCTTGGTGCTGACCTATGTGTTTACCATC
  CTCAACTGCCTGCAGGGCGCCTTCCTCTACCTGCTGCACTGCCTGCTCAACAAGAAGGTT
  CGGGAAGAATACCGGAAGTGGGCCTGCCTAGTTGCTGGGGGGAGCAAGTACTCAGAATTC
  ACCTCCACCACGTCTGGCACTGGCCACAATCAGACCCGGGCCCTCAGGGCATCAGAGTCC
  GGCAYATGA
  
  Clone variation with respect to NM_001784.4
  2225 t=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001784 unedited
  GAACCGTCAAATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGAGACG
  GGACAGCCCTGTCCCACTCACTCTTTCCCCTGCCGCTCCTGCCGGCAGCTCCAACCATGG
  GAGGCCGCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGAAACCCAGG
  ACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGAACTCCTCGTGTGTCAATGCCACCGCCT
  GTCGCTGCAATCCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCGACGGAGACTT
  GTGACGACATCAACGAGTGTGACACACCGTCGAAAGTGTCATGCGGAAAATTCTCGGACT
  GCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCGGGATATGAGCCTGTTTCTG
  GGGCAAAAACATTCAAGAATGAGAGCGAGAACACCTGTCAAGATGTGGACGAGTGCAGCT
  CCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGGGTTCATACAGCT
  GCCGCTGCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGAATAACCAAAAGGACACTG
  TCTGTGAAGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGC
  TTTCCCGATTCTTCGACAAAGTCCAGGACCTGGGCAGAGACTCCAAGACAAGCTCAGCCG
  AGGTCACCATCCAGAATGGTCATCAATTGGGTGGATGAACTGATGGAAGCTCCTGGAAAC
  GTATAGGCCCTGGCGCCACCTGTCCGGCACCTTATAGCCCCCAAGCTGCTCTCAAACCTT
  TGAGAAATCATGAGGATCCCGGCCAAGAACCTGCTTAAGGCCCCCTCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001784
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001784.3, NP_001775.2
• RefSeq Size: 2968 bp
• RefSeq ORF: 2229 bp
• Locus ID: 976
• UniProt ID: P48960
• Cytogenetics: 19p13.12
• Domains: GPS, 7tm_2, EGF_CA, EGF, EGF
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, GPCR, Secreted Protein, Transmembrane
• Gene Summary: This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]
  Transcript Variant: This variant (2) lacks two consecutive exons in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2, also known as CD97(EGF 1,2,5)) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.