Ceramide synthase 2 (CERS2) (NM_022075) Human Untagged Clone

CAT#: SC107203

CERS2 (untagged)-Human LAG1 homolog, ceramide synthase 2 (LASS2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Ceramide synthase 2
• Synonyms: L3; LASS2; SP260; TMSG1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107203 sequence for NM_022075 edited (data generated by NextGen Sequencing)
  ATGCTCCAGACCTTGTATGATTACTTCTGGTGGGAACGTCTGTGGCTGCCTGTGAACTTG
  ACCTGGGCCGATCTAGAAGACCGAGATGGACGTGTCTACGCCAAAGCCTCAGATCTCTAT
  ATCACGCTGCCCCTGGCCTTGCTCTTCCTCATCGTTCGATACTTCTTTGAGCTGTACGTG
  GCTACACCACTGGCTGCCCTCTTGAACATAAAGGAGAAAACTCGGCTGCGGGCACCTCCC
  AACGCCACCTTGGAACATTTCTACCTGACCAGTGGCAAGCAGCCCAAGCAGGTGGAAGTA
  GAGCTTTTGTCCCGGCAGAGCGGGCTCTCTGGCCGCCAGGTAGAGCGTTGGTTCCGTCGC
  CGCCGCAACCAGGACCGGCCCAGTCTCCTCAAGAAGTTCCGAGAAGCCAGCTGGAGATTC
  ACATTTTACCTGATTGCCTTCATTGCCGGCATGGCCGTCATTGTGGATAAACCCTGGTTC
  TATGACATGAAGAAAGTTTGGGAGGGATATCCCATACAGAGCACTATCCCTTCCCAGTAT
  TGGTACTACATGATTGAACTTTCCTTCTACTGGTCCCTGCTCTTCAGCATTGCCTCTGAT
  GTCAAGCGAAAGGATTTCAAGGAACAGATCATCCACCATGTGGCCACCATCATTCTCATC
  AGCTTTTCCTGGTTTGCCAATTACATCCGAGCTGGGACTCTAATCATGGCTCTGCATGAC
  TCTTCCGATTACCTGCTGGAGTCAGCCAAGATGTTTAACTACGCGGGATGGAAGAACACC
  TGCAACAACATCTTCATCGTCTTCGCCATTGTTTTTATCATCACCCGACTGGTCATCCTG
  CCCTTCTGGATCCTGCATTGCACCCTGGTGTACCCACTGGAGCTCTATCCTGCCTTCTTT
  GGCTATTACTTCTTCAATTCCATGATGGGAGTTCTACAGCTGCTGCATATCTTCTGGGCC
  TACCTCATTTTGCGCATGGCCCACAAGTTCATAACTGGAAAGCTGGTAGAAGATGAACGC
  AGTGACCGGGAAGAAACAGAGAGCTCAGAGGGGGAGGAGGCTGCAGCTGGGGGAGGAGCA
  AAGAGCCGGCCCCTAGCCAATGGCCACCCCATCCTCAATAACAACCATCGTAAGAATGAC
  TGA
  
  Clone variation with respect to NM_022075.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022075 unedited
  NGGGCCTGTCAGGATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACCAGGCAG
  CGGCCCCGGCCCCGCCACGCTGCCGCCGGGATGCTCCAGACCTTGTATGATTACTTCTGG
  TGGGAACGTCTGTGGCTGCCTGTGAACTTGACCTGGGCCGATCTAGAAGACCGAGATGGA
  CGTGTCTACGCCAAAGCCTCAGATCTCTATATCACGCTGCCCCTGGCCTTGCTCTTCCTC
  ATCGTTCGATACTTCTTTGAGCTGTACGTGGCTACACCACTGGCTGCCCTCTTGAACATA
  AAGGAGAAAACTCGGCTGCGGGCACCTCCCAACGCCACCTTGGAACATTTCTACCTGACC
  AGTGGCAAGCAGCCCAAGCAGGTGGAAGTAGAGCTTTTGTCCCGGCAGAGCGGGCTCTCT
  GGCCGCCAGGTAGAGCGTTGGTTCCGTCGCCGCCGCAACCAGGACCGGCCCAGTCTCCTC
  AAGAAGTTCCGAGAAGCCAGCTGGAGATTCACATTTTACCTGATTGCCTTCATTGCCGGC
  ATGGCCGTCATTGTGGATAAACCCTGGTTCTATGACATGAAGAAAGTTTGGGAGGGATAT
  CCCATACAGAGCACTATCCCTTCCCAGTATTGGTACTACATGATTGAACTTTCCTTCTAC
  TGGTCCCTGCTCTTCAGCATTGCCTCTGATGTCAAGCGAAAGGATTTCAAGGAACAGATC
  ATCCACCATGTGGCCACCATCATTCTCATCAGCTNTTCCTGGTTTGCCAATTACATCCGA
  GCTGGGACTCTAATCATGGCTCTGCATGACTCTTCCGATTACCTGCTGGAGTCAGCCAAG
  ATGTTTAACTACGCGGGATGGAAGAACACCTGNCACACATCTTCATCGTCTTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022075 unedited
  NGTACCGGTATGTTCACACTTACTCTGAGCAGNANAGCACTGGGGNAGGGTCACAGGGAT
  GCCACCCGGGATCTGTTCAGGAAACAGCTGTGACCGCGGCCGCAATCTAAAGTCGAGTTT
  TTGCGTTTTCTTTTTCTTTTTTTATTTAATATTTTTTATTTAATCTTTTAATTTTAAAAA
  AAAACCCATTAACAGTACATTTTGCTCTAAAATGGGCCCTCTGCTGAAATGCTAGGCGGC
  TCGGCCGGAATTCTGGGTTTAACCCCCCAGCCTCTATCTTTNAGGGATAAGAATTAGAAT
  TAGTTGCGATTCTGCTCGACGCCGGTCTCGCTCAGCCTGGCAGCAGCCGGAAGGGACCCC
  ACCATCCACTCCCATCGCAGGAGCGTCCTAATAGACTGGAAATCCGGGGCAGACAGCCCC
  GTGCGACGCGCCGCTCCGCGCGCAACGAGGCCTCCCACGCCCGGCGTGACAATCCCGTCC
  GCAACCTCCGCCCGTGACCCGCCCTCCGCCACTGCGACCCCGCTTAATACTGCAGTCGCC
  AGCCAACGCCGCCCGAGCGACGCAGCCGGTCCGGACGAGACTGTCCCCCGCAGCGGGCTA
  AGCAATGTCTCAGCAAAACCACGCACAGGTGCTACTCCGCCGCCTACAGCAGGGAGGCGC
  CTACAGTCGCCCCAAACCTCTCGCCCCACCATGCGCCGTTATGGCCACTCGCACTCGCTC
  CGCCGCTCCGCCAGCGCCTACTCATACCACCGCCGCACGCCGACAGATCCGGCCCGCAGG
  CCCCAAGCCACTGACGCCAGCGGACAGCAGAACCGCGGAGCGATCGATCACCCAGCCGGC
  GTCTTCCGCCACACTCACCGCATCGCGTCCGGCACGCCATTCTTCGCTCGTCTCTGATTT
  ACGCGTCCGCTCCATTCGATGCCTGCGGGTGTCCGGTCCTGCCCGCTCCTGCTCGGCGCC
  CGCCACGCCTCCAACGCTGGCTTCACGGTGCTCATCCAAACTGCGAGGGCGTGACTACGC
  GCGTGGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022075
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022075.1, NP_071358.1
• RefSeq Size: 2250 bp
• RefSeq ORF: 1143 bp
• Locus ID: 29956
• UniProt ID: Q96G23
• Cytogenetics: 1q21.3
• Protein Families: Transcription Factors, Transmembrane
• Gene Summary: This gene encodes a protein that has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein may play a role in the regulation of cell growth. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.