Smad2 (NM_001277450) Rat Tagged ORF Clone

CAT#: RR216735

Smad2 (myc-DDK-tagged) - Rat SMAD family member 2 (Smad2), transcript variant 2

Product Images

Circular map for RR216735

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smad2
• Synonyms: Madh2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216735 representing NM_001277450
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCCATCTTGCCATTCACTCCGCCAGTGGTGAAGAGACTTCTGGGATGGAAAAAATCAGCCGGTG
  GGTCTGGAGGAGCAGGTGGTGGAGAACAGAATGGACAGGAAGAAAAGTGGTGTGAAAAAGCAGTGAAAAG
  TCTGGTGAAAAAGCTAAAGAAAACAGGACGATTAGATGAGCTTGAGAAAGCCATCACCACTCAGAATTGC
  AATACTAAGTGTGTCACCATACCAAGCACTTGCTCTGAAATTTGGGGACTGAGTACAGCAAATACGGTAG
  ATCAGTGGGACACAACAGGCCTTTACAGCTTCTCTGAACAAACCAGGTCTCTTGATGGTCGTCTTCAGGT
  GTCTCATCGGAAAGGGCTGCCACATGTTATATATTGCCGGCTGTGGCGCTGGCCAGACCTTCACAGCCAT
  CATGAGCTCAAGGCGATCGAGAACTGCGAATACGCTTTCAGTCTGAAAAAAGATGAAGTGTGTGTGAACC
  CTTACCACTACCAGAGGGTGGAGACACCAGTCTTGCCTCCAGTCTTGGTGCCTCGGCACACAGAGATTCT
  AACAGAACTGCCGCCTCTGGATGACTATACCCACTCCATTCCAGAAAACACAAATTTCCCAGCAGGAATT
  GAGCCACAGAGTAATTACATCCCAGAAACACCACCACCTGGATATATCAGTGAAGATGGAGAAACTAGTG
  ACCAACAGTTGAACCAAAGTATGGACACAGGCTCTCCGGCTGAACTGTCTCCTACCACTCTCTCCCCTGT
  CAATCACAGCTTGGATTTGCAGCCAGTTACTTATTCAGAACCTGCATTTTGGTGTTCAATCGCATATTAT
  GAACTAAACCAGAGGGTTGGAGAGACCTTCCATGCGTCACAGCCCTCACTCACTGTAGACGGCTTTACAG
  ATCCATCGAACTCGGAGAGGTTCTGCTTAGGTTTGCTCTCCAACGTTAACAGAAACGCTACTGTAGAAAT
  GACCAGAAGGCATATAGGAAGGGGAGTGCGCTTGTATTACATAGGTGGGGAAGTGTTTGCCGAGTGCCTA
  AGTGATAGTGCGATCTTTGTGCAGAGCCCCAACTGTAACCAGAGATACGGCTGGCACCCCGCGACAGTGT
  GCAAAATCCCACCAGGCTGTAACCTGAAGATCTTCAACAACCAAGAATTTGCTGCTCTTCTGGCTCAGTC
  TGTTAACCAGGGTTTTGAGGCCGTTTATCAGCTGACTCGAATGTGCACCATAAGAATGAGCTTCGTGAAG
  GGGTGGGGAGCAGAATACCGGAGGCAGACAGTAACAAGTACTCCTTGCTGGATTGAACTTCATCTGAATG
  GCCCCCTGCAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCGTCAGTGCGATGCTCAAGCATGTC
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216735 representing NM_001277450
  Red=Cloning site Green=Tags(s)
  
  MSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNC
  NTKCVTIPSTCSEIWGLSTANTVDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSH
  HELKAIENCEYAFSLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGI
  EPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYY
  ELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECL
  SDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVK
  GWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277450
• ORF Size: 1401 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277450.1, NP_001264379.1
• RefSeq Size: 2598 bp
• RefSeq ORF: 1404 bp
• Locus ID: 29357
• UniProt ID: O70436
• Cytogenetics: 18q12.3
• MW: 52.2 kDa
• Gene Summary: transcriptional mediator for both activin and TGFbeta [RGD, Feb 2006]