Smad2 (NM_001277450) Rat Untagged Clone

CAT#: RN216735

Smad2 (untagged) - Rat SMAD family member 2 (Smad2), transcript variant 2

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Smad2
• Synonyms: Madh2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216735 representing NM_001277450
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCCATCTTGCCATTCACTCCGCCAGTGGTGAAGAGACTTCTGGGATGGAAAAAATCAGCCGGTG
  GGTCTGGAGGAGCAGGTGGTGGAGAACAGAATGGACAGGAAGAAAAGTGGTGTGAAAAAGCAGTGAAAAG
  TCTGGTGAAAAAGCTAAAGAAAACAGGACGATTAGATGAGCTTGAGAAAGCCATCACCACTCAGAATTGC
  AATACTAAGTGTGTCACCATACCAAGCACTTGCTCTGAAATTTGGGGACTGAGTACAGCAAATACGGTAG
  ATCAGTGGGACACAACAGGCCTTTACAGCTTCTCTGAACAAACCAGGTCTCTTGATGGTCGTCTTCAGGT
  GTCTCATCGGAAAGGGCTGCCACATGTTATATATTGCCGGCTGTGGCGCTGGCCAGACCTTCACAGCCAT
  CATGAGCTCAAGGCGATCGAGAACTGCGAATACGCTTTCAGTCTGAAAAAAGATGAAGTGTGTGTGAACC
  CTTACCACTACCAGAGGGTGGAGACACCAGTCTTGCCTCCAGTCTTGGTGCCTCGGCACACAGAGATTCT
  AACAGAACTGCCGCCTCTGGATGACTATACCCACTCCATTCCAGAAAACACAAATTTCCCAGCAGGAATT
  GAGCCACAGAGTAATTACATCCCAGAAACACCACCACCTGGATATATCAGTGAAGATGGAGAAACTAGTG
  ACCAACAGTTGAACCAAAGTATGGACACAGGCTCTCCGGCTGAACTGTCTCCTACCACTCTCTCCCCTGT
  CAATCACAGCTTGGATTTGCAGCCAGTTACTTATTCAGAACCTGCATTTTGGTGTTCAATCGCATATTAT
  GAACTAAACCAGAGGGTTGGAGAGACCTTCCATGCGTCACAGCCCTCACTCACTGTAGACGGCTTTACAG
  ATCCATCGAACTCGGAGAGGTTCTGCTTAGGTTTGCTCTCCAACGTTAACAGAAACGCTACTGTAGAAAT
  GACCAGAAGGCATATAGGAAGGGGAGTGCGCTTGTATTACATAGGTGGGGAAGTGTTTGCCGAGTGCCTA
  AGTGATAGTGCGATCTTTGTGCAGAGCCCCAACTGTAACCAGAGATACGGCTGGCACCCCGCGACAGTGT
  GCAAAATCCCACCAGGCTGTAACCTGAAGATCTTCAACAACCAAGAATTTGCTGCTCTTCTGGCTCAGTC
  TGTTAACCAGGGTTTTGAGGCCGTTTATCAGCTGACTCGAATGTGCACCATAAGAATGAGCTTCGTGAAG
  GGGTGGGGAGCAGAATACCGGAGGCAGACAGTAACAAGTACTCCTTGCTGGATTGAACTTCATCTGAATG
  GCCCCCTGCAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCGTCAGTGCGATGCTCAAGCATGTC
  CTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001277450
• Insert Size: 1404 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277450.1, NP_001264379.1
• RefSeq Size: 2598 bp
• RefSeq ORF: 1404 bp
• Locus ID: 29357
• UniProt ID: O70436
• Cytogenetics: 18q12.3
• Gene Summary: transcriptional mediator for both activin and TGFbeta [RGD, Feb 2006]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.