Sfrp1 (NM_001276712) Rat Tagged ORF Clone

CAT#: RR216324

Sfrp1 (myc-DDK-tagged) - Rat secreted frizzled-related protein 1 (Sfrp1)

Product Images

Circular map for RR216324

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sfrp1
• Synonyms: sFRP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216324 representing NM_001276712
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGTCGGGCGCAACGCGCGGGGTCGCGGCGGGGCCGCCTCCGGCGTGCTGCTGGCGTTGGCCGCCG
  CGCTGCTGGCCGCGGGTTCGGCCAGCGAGTACGACTACGTGAGCTTCCAGTCGGATATCGGCTCCTATCA
  GAGCGGGCGCTTCTACACCAAGCCCCCGCAGTGCGTGGACATCCCGGTGGACCTGCGGCTGTGCCACAAC
  GTGGGCTACAAGAAGATGGTGCTGCCCAACCTGCTGGAGCACGAGACCATGGCAGAGGTGAAGCAGCAGG
  CCAGCAGCTGGGTGCCGCTGCTCAACAAGAACTGCCACATGGGCACCCAGGTCTTCCTCTGCTCGCTCTT
  CGCGCCCGTCTGTCTGGACCGGCCTATCTACCCGTGTCGCTGGCTCTGCGAGGCTGTTCGCGACTCGTGC
  GAGCCGGTCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTCAAATGTGACAAGTTCCCCGAGGGCG
  ACGTCTGCATCGCCATGACCCCGCCCAATGCCACCGAAGCCTCGAAGCCCCAAGGTACAACAGTGTGTCC
  TCCGTGTGACAACGAGTTGAAATCGGAGGCCATCATCGAACATCTCTGTGCAAGCGAGTTTGCGCTGAGA
  ATGAAAATCAAAGAAGTAAAAAAGGAACGGGTGCAAGAGATTGTCCCCAAGAAGAAGAAACCCTTGAAGC
  TGGGGCCCATCAAGAAGAAGGAGCTGAAGCGGCTCGTGCTTTTCCTAAAGAATGGCGCCGACTGTCCCTG
  CCACCAGCTGGACAACCTCAGCCACAACTTCCTCATCATGGGGCGCAAGGTGAAGAGCCAGTACTTGCTC
  ACAGCCATTCACAAGTGGGACAAGAAAAACAAGGAGTTCAAAAACTTCATGAAGAAAATGAAAAACCACG
  AGTGTCCCACCTTCCAGTCTGTTTTTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216324 representing NM_001276712
  Red=Cloning site Green=Tags(s)
  
  MGVGRNARGRGGAASGVLLALAAALLAAGSASEYDYVSFQSDIGSYQSGRFYTKPPQCVDIPVDLRLCHN
  VGYKKMVLPNLLEHETMAEVKQQASSWVPLLNKNCHMGTQVFLCSLFAPVCLDRPIYPCRWLCEAVRDSC
  EPVMQFFGFYWPEMLKCDKFPEGDVCIAMTPPNATEASKPQGTTVCPPCDNELKSEAIIEHLCASEFALR
  MKIKEVKKERVQEIVPKKKKPLKLGPIKKKELKRLVLFLKNGADCPCHQLDNLSHNFLIMGRKVKSQYLL
  TAIHKWDKKNKEFKNFMKKMKNHECPTFQSVFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276712
• ORF Size: 939 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276712.1, NP_001263641.1
• RefSeq Size: 3892 bp
• RefSeq ORF: 942 bp
• Locus ID: 84402
• Cytogenetics: 16q12.5
• MW: 35.4 kDa
• Gene Summary: a secreted protein involved in binding Wnt and antagonizing Wnt signaling [RGD, Feb 2006]