Senp2 (NM_023989) Rat Tagged ORF Clone

CAT#: RR214514

Senp2 (Myc-DDK-tagged ORF) - Rat SUMO1/sentrin/SMT3 specific peptidase 2 (Senp2), (10 ug)

Product Images

Circular map for RR214514

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Senp2
• Synonyms: Axam
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214514 representing NM_023989
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAGATGGCTGACTAAGGTTCTCGGCACCATTCTTCGCTTGTGTGAGCGGCCCGCGCCCGGGGCCC
  GCGCCCTGCTCAAGAGGCGGCGCTCCAGCAGCTCTCTGTTTTCTACTGCAGTGGACACTGATGAAATCCC
  AGCTAAAAGACCAAGACTAGATTGCTTCATTCACCAAGTGAAGAACAGTCTCTACAATGCGGCCAGCTTA
  TTTGGATTCCCATTCCAGCTGACCACCAAACCCATGGTGTCTTCTGCTTGTAATGGAACACGGAATGTGG
  CCCCTTCAGGAGAGGTGTTCTCGAACTCTCCCTCCTGTGAACTGACGACTTCTGGATCCTGCAGCAGCAT
  GCTGAAGCTGGGTAATAAATCTCCTAATGGAATAAGTGACTATCCAAAGATCAGAGTGACAGTGGCCCGA
  GATCAGCCACGGAGAGTCCTGCCTTCCTTTGGTTTTACATTGAAATCAGAAGGCTATAATAGAAGACCAA
  GTGGCCGTCGCCATAGCAAAAGCAATCCAGAGAGTTCCTTACCGTGGAAACCTCAGGAGCAAGGTGTAAC
  AGAGATGATTTCTGAAGAGGGTGGCAAGGGTGCAAGGCGTCCCCATTGCACCGTGGAGGAGGGTGTCCAA
  AAGGACGAAAGAGAAAAATACCTAAAGTTATTGGAGCGTCTTAAAGAGGGTGCTCACGGAAGCACTTTTC
  CTCCTGCAGTTTCACATCATAGTTCTCAGAGAACGCAGATGGACACATTAAAGACCAAAGGCTGGATGGA
  AGAACAGAATCATGGAGTCAGAACAACCCATTTGGTTCCCAAACAGTATAGAGTTGTTGAAACAAGAGGA
  CCTCTCTGTTCAGTGAGAAGTGAAAAGAGGTATTCAAAAGGAAAAGCTGACACAGAGAAGGTGGTGGGAC
  TCCGATTTGAAAAGGACGGTACAAGAGGACACCAGTTGGAGCCTGATCTGTCAGAAGAAGTGTCTGCCCG
  GCTTCGCCTGGGAAGTGGAAGCAATGGCCTACTCAGAAGGAAAATATCAGTGCTTGAGGCAAAGGAAAAG
  AACTTCCCAAGCAAAGAGAAGGACAGAAGAACAGAAGATCTCTTTGAACTTACAGAGGACATGGAAAAGG
  AAATCAGTAATGCCCTGGGCCATGGTCCTCCAGATGAGATTTTGAGCAGCGCTTTCAAGTTACGAATCAC
  CCGTGGAGATATCCAGACCTTAAAGAACTATCACTGGCTCAATGATGAGGTCATTAATTTTTATATGAAT
  CTTTTAGTGGAGAGGAGTAAAAAGCAAGGCTACCCAGCACTTCATGCACTCAGTACATTCTTCTATCCTA
  AATTAAAGTCTGGAGGTTACCAAGCAGTGAAAAGATGGACCAAAGGGGTGAATCTCTTCGATCAAGAACT
  TGTTCTGGTTCCTATTCATCGGAAGGTACATTGGAGCCTGGTGGTGATGGACCTAAGAAAAAAGTGTCTT
  AAATATCTGGATTCTATGGGACAAAAAGGCCACAGGATCTGTGAGATTCTTCTTCAGTATTTACAGGACG
  AAAGCAAGACCAAAAGAAATACAGATCTCAATCTCTTAGAGTGGACCCACTACAGCATGAAGCCACATGA
  GATTCCTCAACAGTTGAATGGGAGTGATTGTGGAATGTTTACTTGTAAATACGCAGATTATATTTCTAGG
  GACAAACCTATCACATTCACTCAGCACCAGATGCCTCTCTTCCGGAAGAAGATGGTGTGGGAAATCCTTC
  ACCAGCAGTTGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214514 representing NM_023989
  Red=Cloning site Green=Tags(s)
  
  MYRWLTKVLGTILRLCERPAPGARALLKRRRSSSSLFSTAVDTDEIPAKRPRLDCFIHQVKNSLYNAASL
  FGFPFQLTTKPMVSSACNGTRNVAPSGEVFSNSPSCELTTSGSCSSMLKLGNKSPNGISDYPKIRVTVAR
  DQPRRVLPSFGFTLKSEGYNRRPSGRRHSKSNPESSLPWKPQEQGVTEMISEEGGKGARRPHCTVEEGVQ
  KDEREKYLKLLERLKEGAHGSTFPPAVSHHSSQRTQMDTLKTKGWMEEQNHGVRTTHLVPKQYRVVETRG
  PLCSVRSEKRYSKGKADTEKVVGLRFEKDGTRGHQLEPDLSEEVSARLRLGSGSNGLLRRKISVLEAKEK
  NFPSKEKDRRTEDLFELTEDMEKEISNALGHGPPDEILSSAFKLRITRGDIQTLKNYHWLNDEVINFYMN
  LLVERSKKQGYPALHALSTFFYPKLKSGGYQAVKRWTKGVNLFDQELVLVPIHRKVHWSLVVMDLRKKCL
  KYLDSMGQKGHRICEILLQYLQDESKTKRNTDLNLLEWTHYSMKPHEIPQQLNGSDCGMFTCKYADYISR
  DKPITFTQHQMPLFRKKMVWEILHQQLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023989
• ORF Size: 1764 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023989.1, NP_076479.1
• RefSeq Size: 1808 bp
• RefSeq ORF: 1767 bp
• Locus ID: 78973
• UniProt ID: Q9EQE1
• Cytogenetics: 11q23
• MW: 67.3 kDa
• Gene Summary: binds the GSK-3beta interacting protein Axin; inhibits the Wnt signalling pathway via inhibition of Axin protein complex formation [RGD, Feb 2006]