Fads1 (NM_053445) Rat Tagged ORF Clone
CAT#: RR214038
- TrueORF®
Fads1 (Myc-DDK-tagged ORF) - Rat fatty acid desaturase 1 (Fads1), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_053445" in other vectors (3)
USD 198.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Fads1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR214038 representing NM_053445
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCCGACCCGGTGCAGACCCCTGACCCGGCCTCCGCCCAGCTCCGCCAAATGCGCTACTTTACTT GGGAGGAGGTGGCGCAGCGCTCCGGGAGGGAGAAGGAGCGATGGCTCGTGATCGACCGGAAGGTGTACAA CATCAGCGACTTCAGTCGCCGCCACCCGGGAGGCTCCCGGGTCATCAGCCACTACGCTGGTCAGGATGCC ACGGATCCTTTTGTGGCATTCCACATTAACAAGGGCCTTGTGAGAAAGTATATGAACTCTCTTCTGATTG GAGAGCTAGCTCCGGAGCAGCCCAGCTTTGAACCCACCAAGAATAAGGCGCTCACTGATGAATTCCGGGA GCTGCGGGCCACAGTGGAGCGAATGGGCCTCATGAAAGCCAACCATCTCTTCTTCCTGTTCTATCTGCTG CACATCCTGCTGCTGGACGTGGCCGCCTGGCTCACTCTTTGGATCTTTGGAACTTCCTTGGTGCCCTTCA CCCTCTGTGCAGTGCTGCTCAGTACAGTTCAGGCCCAGGCAGGTTGGCTACAGCATGACTTTGGGCACCT GTCCGTCTTCAGCACCTCAACATGGAATCACCTGGTACATCATTTTGTCATTGGCCACCTGAAGGGGGCC CCAGCCAGCTGGTGGAACCACATGCATTTCCAGCACCACGCCAAGCCCAACTGCTTCCGCAAAGACCCCG ATATCAACATGCATCCCCTCTTCTTCGCCCTGGGGAAGGTCCTTTCTGTGGAGCTTGGGAAAGAAAAGAA GAAGCACATGCCATACAACCATCAGCACAAGTACTTCTTCCTGATTGGACCCCCAGCCTTGCTGCCTCTC TACTTCCAGTGGTACATTTTCTATTTTGTTGTTCAGCGGAAGAAATGGGTGGACTTGGCCTGGATGCTCA GCTTCTATGTTCGTGTCTTCTTCACTTACATGCCGCTGCTGGGGCTGAAAGGCCTCCTATGTCTTTTCTT CATTGTCAGGTTCCTGGAGAGCAACTGGTTTGTGTGGGTGACGCAGATGAACCATATCCCCATGCACATT GATCATGACCGGAATGTGGACTGGGTCTCCACCCAGCTACAGGCAACCTGCAACGTTCACCAGTCAGCCT TCAACAACTGGTTCAGTGGCCACCTCAATTTCCAGATTGAACACCACCTCTTCCCTACGATGCCACGACA CAACTACCACAAGGTGGCACCCCTGGTACAATCTCTGTGCGCCAAGTACGGCATCAAGTATGAGTCCAAG CCCCTGCTCACGGCCTTCGCGGACATTGTTTACTCCCTGAAGGAGTCAGGACAGCTCTGGCTGGATGCTT ACCTTCACCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214038 representing NM_053445
Red=Cloning site Green=Tags(s) MAPDPVQTPDPASAQLRQMRYFTWEEVAQRSGREKERWLVIDRKVYNISDFSRRHPGGSRVISHYAGQDA TDPFVAFHINKGLVRKYMNSLLIGELAPEQPSFEPTKNKALTDEFRELRATVERMGLMKANHLFFLFYLL HILLLDVAAWLTLWIFGTSLVPFTLCAVLLSTVQAQAGWLQHDFGHLSVFSTSTWNHLVHHFVIGHLKGA PASWWNHMHFQHHAKPNCFRKDPDINMHPLFFALGKVLSVELGKEKKKHMPYNHQHKYFFLIGPPALLPL YFQWYIFYFVVQRKKWVDLAWMLSFYVRVFFTYMPLLGLKGLLCLFFIVRFLESNWFVWVTQMNHIPMHI DHDRNVDWVSTQLQATCNVHQSAFNNWFSGHLNFQIEHHLFPTMPRHNYHKVAPLVQSLCAKYGIKYESK PLLTAFADIVYSLKESGQLWLDAYLHQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_053445 |
ORF Size | 1341 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053445.2, NP_445897.2 |
RefSeq Size | 3380 bp |
RefSeq ORF | 1344 bp |
Locus ID | 84575 |
UniProt ID | Q920R3 |
Cytogenetics | 1q43 |
MW | 52.5 kDa |
Gene Summary | catalyzes the Delta(5,6) desaturation of fatty acids of the n-6 and n-3 series [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN214038 | Fads1 (untagged ORF) - Rat fatty acid desaturase 1 (Fads1), (10 ug) |
USD 503.00 |
|
RR214038L3 | Lenti ORF clone of Fads1 (Myc-DDK-tagged ORF) - Rat fatty acid desaturase 1 (Fads1), (10 ug) |
USD 803.00 |
|
RR214038L4 | Lenti ORF clone of Fads1 (mGFP-tagged ORF) - Rat fatty acid desaturase 1 (Fads1), (10 ug) |
USD 803.00 |
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