Pdha1 (NM_001004072) Rat Tagged ORF Clone

CAT#: RR210845

Pdha1 (Myc-DDK-tagged ORF) - Rat pyruvate dehydrogenase (lipoamide) alpha 1 (Pdha1), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR210845

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pdha1
• Synonyms: MGC94854; MGC114215
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210845 representing NM_001004072
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAGATGCTTGCCGCTGTGTCCCGCGTGTTGGCAGGCGCTGCGCAGAAGCCGGCAAGCAGAGTGC
  TGGTAGCTTCCCGTAATTTCGCAAATGATGCTACATTTGAGATTAAGAAATGTGACCTTCATCGTCTAGA
  AGAGGGCCCTCCAGTCACCACAGTGCTCACCAGAGAGGATGGGCTCAAGTACTACAGGATGATGCAGACC
  GTGCGCCGGATGGAGCTCAAGGCGGATCAGCTGTATAAGCAGAAAATTATTCGTGGTTTCTGTCACTTGT
  GTGATGGTCAGGAAGCCTGCTGTGTGGGCCTGGAGGCTGGCATAAACCCCACGGACCATCTCATCACTGC
  CTATCGAGCCCATGGCTTCACCTTCACTCGGGGCCTGCCTGTCCGAGCAATTCTTGCAGAATTAACAGGA
  CGAAGAGGAGGCTGTGCTAAGGGGAAAGGCGGATCAATGCACATGTACGCCAAGAACTTCTATGGCGGCA
  ACGGTATCGTTGGAGCTCAGGTGCCCCTGGGTGCAGGAATTGCTCTGGCCTGCAAGTATAATGGCAAAGA
  TGAGGTCTGTTTGACATTATATGGTGATGGTGCTGCTAACCAGGGTCAGATATTTGAAGCTTACAATATG
  GCAGCATTGTGGAAATTACCTTGTATTTTCATCTGTGAGAATAACCGCTATGGCATGGGCACATCTGTTG
  AGAGAGCAGCAGCCAGCACGGATTACTACAAAAGAGGAGATTTCATTCCTGGACTGAGGGTAGATGGAAT
  GGATATCTTGTGTGTCCGAGAGGCAACAAAGTTTGCAGCTGCCTACTGCAGGTCTGGTAAGGGGCCTATC
  CTGATGGAGCTACAGACTTACCGCTACCATGGACATAGTATGAGTGACCCTGGAGTCAGTTATCGCACTC
  GAGAAGAAATCCAGGAAGTAAGAAGTAAGAGTGACCCTATTATGCTTCTCAAGGATAGAATGGTGAACAG
  CAATCTTGCAAGTGTTGAAGAATTAAAGGAGATTGATGTGGAAGTGAGGAAAGAAATCGAGGATGCTGCC
  CAGTTTGCCACGGCTGATCCGGAGCCGCCATTGGAGGAGCTAGGCTATCATATCTACAGCAGTGACCCAC
  CCTTCGAAGTGCGTGGTGCCAACCAGTGGATCAAGTTTAAGTCAGTCAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210845 representing NM_001004072
  Red=Cloning site Green=Tags(s)
  
  MRKMLAAVSRVLAGAAQKPASRVLVASRNFANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQT
  VRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLPVRAILAELTG
  RRGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNM
  AALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATKFAAAYCRSGKGPI
  LMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAA
  QFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004072
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004072.2, NP_001004072.2
• RefSeq Size: 2839 bp
• RefSeq ORF: 1173 bp
• Locus ID: 29554
• Cytogenetics: Xq14
• MW: 43.2 kDa
• Gene Summary: catalyzes the interconversion of pyruvate and lipoamide to S-acetyldihydrolipoamide and CO2 [RGD, Feb 2006]