Pdha1 (NM_001004072) Rat Untagged Clone

CAT#: RN210845

Pdha1 (untagged ORF) - Rat pyruvate dehydrogenase (lipoamide) alpha 1 (Pdha1), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pdha1
• Synonyms: MGC94854; MGC114215
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210845 representing NM_001004072
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAGATGCTTGCCGCTGTGTCCCGCGTGTTGGCAGGCGCTGCGCAGAAGCCGGCAAGCAGAGTGC
  TGGTAGCTTCCCGTAATTTCGCAAATGATGCTACATTTGAGATTAAGAAATGTGACCTTCATCGTCTAGA
  AGAGGGCCCTCCAGTCACCACAGTGCTCACCAGAGAGGATGGGCTCAAGTACTACAGGATGATGCAGACC
  GTGCGCCGGATGGAGCTCAAGGCGGATCAGCTGTATAAGCAGAAAATTATTCGTGGTTTCTGTCACTTGT
  GTGATGGTCAGGAAGCCTGCTGTGTGGGCCTGGAGGCTGGCATAAACCCCACGGACCATCTCATCACTGC
  CTATCGAGCCCATGGCTTCACCTTCACTCGGGGCCTGCCTGTCCGAGCAATTCTTGCAGAATTAACAGGA
  CGAAGAGGAGGCTGTGCTAAGGGGAAAGGCGGATCAATGCACATGTACGCCAAGAACTTCTATGGCGGCA
  ACGGTATCGTTGGAGCTCAGGTGCCCCTGGGTGCAGGAATTGCTCTGGCCTGCAAGTATAATGGCAAAGA
  TGAGGTCTGTTTGACATTATATGGTGATGGTGCTGCTAACCAGGGTCAGATATTTGAAGCTTACAATATG
  GCAGCATTGTGGAAATTACCTTGTATTTTCATCTGTGAGAATAACCGCTATGGCATGGGCACATCTGTTG
  AGAGAGCAGCAGCCAGCACGGATTACTACAAAAGAGGAGATTTCATTCCTGGACTGAGGGTAGATGGAAT
  GGATATCTTGTGTGTCCGAGAGGCAACAAAGTTTGCAGCTGCCTACTGCAGGTCTGGTAAGGGGCCTATC
  CTGATGGAGCTACAGACTTACCGCTACCATGGACATAGTATGAGTGACCCTGGAGTCAGTTATCGCACTC
  GAGAAGAAATCCAGGAAGTAAGAAGTAAGAGTGACCCTATTATGCTTCTCAAGGATAGAATGGTGAACAG
  CAATCTTGCAAGTGTTGAAGAATTAAAGGAGATTGATGTGGAAGTGAGGAAAGAAATCGAGGATGCTGCC
  CAGTTTGCCACGGCTGATCCGGAGCCGCCATTGGAGGAGCTAGGCTATCATATCTACAGCAGTGACCCAC
  CCTTCGAAGTGCGTGGTGCCAACCAGTGGATCAAGTTTAAGTCAGTCAGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001004072
• Insert Size: 1173 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004072.2, NP_001004072.2
• RefSeq Size: 2839 bp
• RefSeq ORF: 1173 bp
• Locus ID: 29554
• Cytogenetics: Xq14
• Gene Summary: catalyzes the interconversion of pyruvate and lipoamide to S-acetyldihydrolipoamide and CO2 [RGD, Feb 2006]