Arntl (NM_024362) Rat Tagged ORF Clone

CAT#: RR210481

Arntl (Myc-DDK-tagged ORF) - Rat aryl hydrocarbon receptor nuclear translocator-like (Arntl), (10 ug)

Product Images

Circular map for RR210481

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arntl
• Synonyms: Bmal1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210481 representing NM_024362
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACCAGAGAATGGACATTTCCTCCACAATCAGTGACTTCATGTCTCCGGGCCCCACCGACCTGC
  TCTCCGGTTCCCTGAGCACCAGTGGTGTGGACTGCAACCGCAAGAGGAAAGGCAGTGCCACTGACTACCA
  AGAAAGTATGGACACAGACAAAGATGACCCTCATGGAAGGTTAGAATATGCAGAACACCAAGGAAGGATC
  AAAAATGCAAGGGAGGCCCACAGTCAGATTGAAAAGAGGCGTCGGGACAAAATGAACAGTTTTATTGACG
  AATTGGCTTCTTTGGTACCAACATGCAATGCGATGTCCCGGAAGTTAGATAAACTCACCGTGCTCAGGAT
  GGCTGTTCAGCACATGAAAACATTGAGAGGTGCCACCAACCCATACACAGAAGCAAACTACAAGCCAACA
  TTTCTATCCGATGACGAACTGAAACACCTAATTCTCAGGGCAGCAGATGGATTTTTGTTTGTCGTAGGAT
  GTGACCGAGGGAAGATCCTCTTTGTCTCCGAATCTGTCTTCAAGATCCTCAATTATAGCCAGAATGACCT
  TATTGGCCAGAGTTTGTTCGACTACCTGCATCCAAAAGATATTGCCAAAGTTAAGGAACAGCTATCTTCC
  TCGGACACTGCACCTCGGGAGCGACTCATCGATGCGAAGACTGGACTTCCGGTTAAAACGGATATAACCC
  CTGGGCCCTCCCGATTGTGCTCTGGAGCTCGGCGCTCTTTCTTCTGTAGGATGAAGTGCAACAGGCCTTC
  AGTAAAGGTGGAAGATAAGGACTTCGCCTCCACCTGTTCAAAGAAAAAAGCAGATCGAAAGAGCTTCTGC
  ACAATCCACAGCACAGGCTACTTGAAAAGCTGGCCACCCACAAAGATGGGGCTGGACGAAGACAGTGAAC
  CAGACAATGAGGGCTGCAATCTGAGCTGCCTCGTTGCAATCGGGCGACTGCACTCACACATGGTTCCACA
  GCCAGTGAACGGGGAAATACGGGTGAAGTCTATGGAGTATGTGTCTCGACATGCAATAGATGGGAAATTT
  GTTTTTGTAGATCAGAGGGCGACGGCTATTTTGGCATATCTACCACAGGAACTTCTAGGTACATCGTGTT
  ATGAGTATTTTCATCAAGATGATATAGGACACCTTGCGGAATGTCACAGGCAAGTTTTACAGACAAGAGA
  AAAGATCACGACTAATTGCTATAAGTTTAAGATCAAAGATGGTTCCTTTATCACACTACGAAGTCGATGG
  TTCAGTTTCATGAACCCGTGGACCAAGGAAGTAGAATACATTGTCTCAACCAACACTGTTGTTTTAGCCA
  ATGTCCTGGAAGGCGGGGACCCAACCTTCCCACAGCTCACAGCATCCCCCCACAGCATGGACAGCATGCT
  GCCCTCTGGAGAAGGTGGCCCAAAGAGGACTCATCCCACTGTTCCAGGCATTCCAGGGGGAACCAGAGCT
  GGAGCAGGAAAAATAGGTCGAATGATTGCCGAGGAAATCATGGAAATCCACAGGATAAGAGGGTCATCAC
  CTTCCAGCTGTGGCTCCAGCCCACTGAACATCACAAGTACGCCTCCCCCTGATGCTTCCTCTCCAGGAGG
  CAAGAAGATTCTAAATGGAGGGACTCCAGACATTCCTTCCGCTGGCCTACTACCAGGGCAGGCTCAGGAG
  ACCCCAGGCTATCCCTACTCTGATAGTTCGTCTATTCTTGGTGAGAACCCCCACATAGGCATCGACATGA
  TAGACAACGACCAAGGATCAAGCAGCCCCAGTAACGACGAGGCAGCAATGGCTGTCATCATGAGCCTCTT
  GGAAGCAGATGCGGGGCTGGGTGGTCCCGTTGACTTTAGTGACTTGCCATGGCCACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210481 representing NM_024362
  Red=Cloning site Green=Tags(s)
  
  MADQRMDISSTISDFMSPGPTDLLSGSLSTSGVDCNRKRKGSATDYQESMDTDKDDPHGRLEYAEHQGRI
  KNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRKLDKLTVLRMAVQHMKTLRGATNPYTEANYKPT
  FLSDDELKHLILRAADGFLFVVGCDRGKILFVSESVFKILNYSQNDLIGQSLFDYLHPKDIAKVKEQLSS
  SDTAPRERLIDAKTGLPVKTDITPGPSRLCSGARRSFFCRMKCNRPSVKVEDKDFASTCSKKKADRKSFC
  TIHSTGYLKSWPPTKMGLDEDSEPDNEGCNLSCLVAIGRLHSHMVPQPVNGEIRVKSMEYVSRHAIDGKF
  VFVDQRATAILAYLPQELLGTSCYEYFHQDDIGHLAECHRQVLQTREKITTNCYKFKIKDGSFITLRSRW
  FSFMNPWTKEVEYIVSTNTVVLANVLEGGDPTFPQLTASPHSMDSMLPSGEGGPKRTHPTVPGIPGGTRA
  GAGKIGRMIAEEIMEIHRIRGSSPSSCGSSPLNITSTPPPDASSPGGKKILNGGTPDIPSAGLLPGQAQE
  TPGYPYSDSSSILGENPHIGIDMIDNDQGSSSPSNDEAAMAVIMSLLEADAGLGGPVDFSDLPWPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024362
• ORF Size: 1878 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024362.2, NP_077338.2
• RefSeq Size: 2991 bp
• RefSeq ORF: 1881 bp
• Locus ID: 29657
• UniProt ID: Q9EPW1
• Cytogenetics: 1q33
• MW: 68.7 kDa
• Gene Summary: The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with Clock. This heterodimer binds E-box enhancer elements upstream of Period (Per1, Per2, Per3) and Cryptochrome (Cry1, Cry2) genes and activates transcription of these genes. Per and Cry proteins heterodimerize and repress their own transcription by interacting in a feedback loop with Clock/Arntl complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. [provided by RefSeq, Jan 2014]