Abcd4 (NM_001013100) Rat Tagged ORF Clone

CAT#: RR209475

Abcd4 (Myc-DDK-tagged ORF) - Rat ATP-binding cassette, sub-family D (ALD), member 4 (Abcd4), (10 ug)

Product Images

Circular map for RR209475

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcd4
• Synonyms: Pxmp1l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209475 representing NM_001013100
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTCCCGGGACCCACGGCCCGAGCTAGTGCCAGGCCCAGATTAGATCTGCAACTTGTCCAGAAGT
  TCATAAGGATACAGAAGGTTTTCTTTCCTTCTTGGTCATCACAGAATGTCTTAATGTTCATGACACTCTT
  GTGTGTGGCCCTCCTGGAGCAACTGGTGATCTACCAAGTTGGCTTGATCCCCAGTCAGTATTATGGTGTC
  TTGGGAAACAAAGACCTGGATGGATTTAAGGCACTGACCTTCCTGGCCGTGGCACTCATTGTTCTCAACT
  CCACCCTGAAGAGTTTTGACCAGTTCACCTGCAACCTGCTGTATGTGAGCTGGAGGAAAGACCTCACAGA
  GCACCTGCACCAGCTCTACTTCCGGGCCCGCGTGTACTACACCCTCAACGTGCTGCGGGACGACATCGAT
  AATCCGGACCAGCGGATCAGCCAGGACGTGGAGCGGTTCTGCCGGCAGCTCAGCAGCGTGACCAGCAAGC
  TCATCATCTCCCCCTTCACTCTCGCCTACTATACCTACCAGTGCTTCCAAAGCACAGGCTGGCTTGGGCC
  TGTGAGCATCTTTGGATATTTCATCCTGGGTACCATGGTGAACAAAACTTTGATGGGGCCCATCGTGACG
  AAGCTGGTGCAGCAGGAGAAGCTGGAGGGGGATTTTAGGTTCAAGCATATGCAGATTCGAGTGAACGCTG
  AGCCTGCAGCTTTCTACAGAGCTGGGCTCGTAGAGCACATGAGGACGGACCGCAGGCTGCAGAGACTCCT
  TCAGACCCAGAGAGAGCTGATGTCCAGGGAGCTCTGGCTCTACATTGGCATCAACACCTTTGACTACCTG
  GGCAGCATTCTGAGTTACGTCGTCATCGCCATCCCCATTTTCAGGGGGGTCTACGGAGACTTGAGCCCCA
  CAGAGCTCAGCACCCTGGTCAGCAAGAATGCCTTTGTGTGCATCTACCTCATCAGCTGCTTCACCCAGCT
  CATTGACCTGTCCACCACGCTCTCGGATGTCGCCGGTTACACGCACAGGATTGGAGAGCTTCAGGAGGCC
  CTGCTGGACATGTCCCGAAAATCACACGACTGTGAGACCCTAGGCGAGAGCGAGTGGGATTTGGACAAGG
  CCCCAGGGTGGCCGACAACAGAGCCATCAGACACTGCTTTTCTGCTTGATCGGGTCTCCATCTTGGCCCC
  TTCCTCCGACAAGCCCCTGATCAAGGACTTGAGCCTGAAGATCTGTGAGGGGCAGAGTCTGCTCATTACG
  GGCAACACAGGCACTGGCAAGACCTCCCTGCTGCGGGTGCTGGGAGGCCTGTGGGAGAGCATGAAAGGCT
  CAGTGCAGATGCTGGCTGATTTTGGGCCCCACGGGGTGCTGTTCCTGCCTCAGAAGCCGTTCTTCACTGA
  TGGGACACTTCGGGAGCAGGTGATATATCCCCTGAAGGAGATCTACCCTGACTCAGGTTCTGCCGATGAT
  GAGAGGATCGTGAGGTTCTTGGAGTTGGCAGGCCTGTCCAGCTTGGTGGCAAGAACAGGAGGTCTGGACC
  AGCAGGTGGATTGGAACTGGTATGATGTCCTGTCCCCAGGGGAGATGCAAAGACTGTCCTTCGCCCGCCT
  CTTCTACCTGCAGCCTAAGTATGCAGTGCTTGATGAAGCCACCAGTGCCCTGACAGAGGAAGCGGAGAGT
  GAGCTCTACCGAATTGGCCAGCAACTGGGGATGACATTTATCAGCGTGGGACATCGGCCCAGCCTTGAGA
  AGTTTCACTCCTGGGTTCTGAGACTCCATGGAGGAGGAAGCTGGGAACTGACTAGAATCAAAATGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209475 representing NM_001013100
  Red=Cloning site Green=Tags(s)
  
  MAVPGPTARASARPRLDLQLVQKFIRIQKVFFPSWSSQNVLMFMTLLCVALLEQLVIYQVGLIPSQYYGV
  LGNKDLDGFKALTFLAVALIVLNSTLKSFDQFTCNLLYVSWRKDLTEHLHQLYFRARVYYTLNVLRDDID
  NPDQRISQDVERFCRQLSSVTSKLIISPFTLAYYTYQCFQSTGWLGPVSIFGYFILGTMVNKTLMGPIVT
  KLVQQEKLEGDFRFKHMQIRVNAEPAAFYRAGLVEHMRTDRRLQRLLQTQRELMSRELWLYIGINTFDYL
  GSILSYVVIAIPIFRGVYGDLSPTELSTLVSKNAFVCIYLISCFTQLIDLSTTLSDVAGYTHRIGELQEA
  LLDMSRKSHDCETLGESEWDLDKAPGWPTTEPSDTAFLLDRVSILAPSSDKPLIKDLSLKICEGQSLLIT
  GNTGTGKTSLLRVLGGLWESMKGSVQMLADFGPHGVLFLPQKPFFTDGTLREQVIYPLKEIYPDSGSADD
  ERIVRFLELAGLSSLVARTGGLDQQVDWNWYDVLSPGEMQRLSFARLFYLQPKYAVLDEATSALTEEAES
  ELYRIGQQLGMTFISVGHRPSLEKFHSWVLRLHGGGSWELTRIKMG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013100
• ORF Size: 1818 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013100.3, NP_001013118.2
• RefSeq Size: 2358 bp
• RefSeq ORF: 1821 bp
• Locus ID: 299196
• Cytogenetics: 6q31
• MW: 68.7 kDa