Ptk2b (NM_017318) Rat Tagged ORF Clone

CAT#: RR207973

Ptk2b (Myc-DDK-tagged ORF) - Rat PTK2B protein tyrosine kinase 2 beta (Ptk2b), (10 ug)

Product Images

Circular map for RR207973

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptk2b
• Synonyms: CAKB; CAK beta; CAKbeta; Pyk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207973 representing NM_017318
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGGGTGTCTGAGCCCCTGAGCCGTGTAAAAGTGGGCACTTTGCGCCCGCCCGAGGGCCCCCCAG
  AGCCCATGGTAGTGGTACCAGTAGACGTGGAGAAGGAAGACGTGCGTATCCTCAAGGTCTGCTTCTACAG
  CAACAGCTTCAACCCAGGGAAGAACTTCAAGCTTGTCAAATGCACAGTGCAGACAGAGATCCAGGAGATC
  ATCACCTCCATCCTCCTGAGTGGGCGAATAGGGCCCAACATCCAGCTGGCTGAATGCTATGGGCTGAGGC
  TGAAGCACATGAAGTCAGATGAGATCCACTGGCTGCACCCACAGATGACTGTGGGCGAAGTGCAGGACAA
  GTATGAATGTCTACACGTGGAGGCTGAGTGGAGGTATGACCTTCAAATCCGCTATTTGCCAGAAGACTTC
  ATGGAGAGCCTGAAAGAAGACAGGACTACACTGCTGTACTTTTATCAACAGCTCCGGAATGACTACATGC
  AACGCTATGCCAGCAAGGTCAGTGAAGGCATGGCTCTGCAGCTGGGCTGTCTGGAGCTCAGGAGATTCTT
  CAAGGACATGCCCCACAATGCACTTGACAAAAAGTCCAACTTTGAACTCCTGGAAAAAGAAGTCGGTCTG
  GACCTGTTTTTCCCAAAGCAAATGCAGGAAAACTTAAAGCCCAAGCAGTTCCGGAAGATGATCCAGCAGA
  CCTTCCAGCAGTATGCATCACTCCGGGAGGAAGAGTGTGTCATGAAATTCTTCAATACTCTGGCAGGCTT
  TGCCAACATCGACCAGGAGACCTATCGCTGTGAACTCATTCAAGGGTGGAACATTACTGTGGACCTGGTC
  ATTGGCCCTAAAGGCATCCGTCAGCTGACAAGTCAAGACACAAAGCCCACCTGCCTCGCCGAGTTCAAGC
  AGATCAGATCCATCAGGTGCCTCCCCTTGGAAGAGACCCAGGCAGTACTGCAGCTGGGCATCGAGGGTGC
  CCCCCAGTCCTTGTCCATCAAAACCTCGTCCCTGGCAGAGGCTGAGAACATGGCTGACCTCATAGATGGC
  TACTGCAGGCTTCAAGGAGAGCATAAAGGCTCTCTCATCATTCATGCCAAGAAAGATGGTGAGAAGAGGA
  ACAGCCTGCCTCAGATCCCCACACTAAACCTGGAGTCTCGGCGGTCCCACCTCTCAGAAAGCTGTAGCAT
  AGAGTCAGACATCTATGCAGAGATTCCTGATGAGACCCTGCGAAGGCCAGGAGGCCCACAGTATGGTGTT
  GCCCGTGAAGATGTAGTTCTTAACCGCATTCTGGGTGAAGGCTTCTTCGGGGAGGTCTACGAAGGTGTCT
  ACACGAACCATAAAGGGGAAAAAATTAATGTGGCCGTCAAGACCTGTAAGAAAGATTGTACCCTGGACAA
  CAAGGAGAAGTTCATGAGTGAGGCAGTGATCATGAAGAATCTTGACCACCCTCACATCGTCAAGCTGATT
  GGCATCATTGAAGAGGAACCCACATGGATCGTCATGGAACTGTATCCTTATGGGGAGCTGGGACACTACC
  TGGAGCGAAATAAAAATTCCCTGAAGGTACCCACTCTGGTCCTGTACGCTCTGCAGATATGCAAAGCCAT
  GGCCTATCTGGAGAGCATCAACTGTGTCCACAGGGATATTGCTGTCCGGAACATCCTGGTGGCCTCTCCT
  GAGTGTGTGAAGCTCGGGGACTTTGGGCTCTCCCGGTACATTGAGGATGAGGACTATTACAAAGCTTCCG
  TGACCCGTCTCCCCATCAAATGGATGTCCCCCGAGTCCATCAACTTTCGCCGCTTTACAACCGCCAGTGA
  CGTCTGGATGTTTGCTGTATGCATGTGGGAGATCCTCAGCTTTGGGAAGCAGCCTTTCTTCTGGCTTGAA
  AACAAGGATGTCATCGGGGTGCTGGAGAAAGGGGACAGGCTGCCTAAGCCCGAACTCTGCCCGCCTGTCC
  TTTACACACTCATGACCCGCTGCTGGGACTATGACCCCAGCGACCGGCCCCGCTTTACGGAGCTTGTGTG
  CAGTCTCAGTGACATTTATCAGATGGAGAGGGACATTGCAATAGAACAAGAAAGGAACGCTCGTTACCGA
  CCCCCCAAAATATTGGAGCCCACTGCTTTTCAGGAACCCCCACCCAAGCCCAGCCGGCCCAAGTACAAAC
  ACCCTCCACAGACCAATCTGCTGGCTCCTAAGCTGCAGTTCCAGGTCCCTGAGGGTCTGTGTGCCAGCTC
  TCCTACACTTACGAGCCCTATGGAGTATCCATCTCCAGTTAACTCGCTGCACACCCCGCCTCTGCACCGA
  CACAATGTCTTCAAGCGCCACAGCATGCGGGAGGAGGACTTCATCCGGCCCAGTAGTCGAGAAGAGGCCC
  AGCAGCTCTGGGAGGCAGAGAAGATCAAGATGAGGCAGGTCCTAGACAGACAGCAGAAGCAAATGGTGGA
  AGATTCCCAGTGGCTGAGGCGGGAGGAAAGATGCTTGGACCCGATGGTTTATATGAATGACAAGTCCCCA
  CTGACCCCAGAGAAGGAGGCCGGCTACACGGAGTTCACAGGGCCCCCCCAGAAGCCACCACGGCTCGGTG
  CACAGTCCATCCAGCCTACAGCCAACTTGGACAGGACTGATGACCTCGTGTACCACAATGTCATGACCCT
  GGTGGAGGCCGTGCTGGAACTCAAGAACAAGCTCAGCCAGCTGCCTCCTGAGGAGTATGTGGTGGTGGTG
  AAGAATGTGGGGCTGAACCTGCGGAAGCTCATCGGTAGCGTGGACGATCTCCTACCCTCCTTGCCAGCAT
  CTTCTAGAACAGAGATTGAAGGGACCCAGAAACTGCTCAACAAAGACCTGGCAGAGCTCATCAACAAGAT
  GAGGTTGGCTCAGCAGAACGCTGTGACATCCCTAAGTGAGGACTGCAAGCGGCAGATGCTCACAGCATCC
  CATACCCTGGCTGTGGATGCCAAGAACCTCCTGGATGCTGTAGACCAAGCCAAGGTTGTGGCTAATCTGG
  CCCACCCGCCTGCAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207973 representing NM_017318
  Red=Cloning site Green=Tags(s)
  
  MSGVSEPLSRVKVGTLRPPEGPPEPMVVVPVDVEKEDVRILKVCFYSNSFNPGKNFKLVKCTVQTEIQEI
  ITSILLSGRIGPNIQLAECYGLRLKHMKSDEIHWLHPQMTVGEVQDKYECLHVEAEWRYDLQIRYLPEDF
  MESLKEDRTTLLYFYQQLRNDYMQRYASKVSEGMALQLGCLELRRFFKDMPHNALDKKSNFELLEKEVGL
  DLFFPKQMQENLKPKQFRKMIQQTFQQYASLREEECVMKFFNTLAGFANIDQETYRCELIQGWNITVDLV
  IGPKGIRQLTSQDTKPTCLAEFKQIRSIRCLPLEETQAVLQLGIEGAPQSLSIKTSSLAEAENMADLIDG
  YCRLQGEHKGSLIIHAKKDGEKRNSLPQIPTLNLESRRSHLSESCSIESDIYAEIPDETLRRPGGPQYGV
  AREDVVLNRILGEGFFGEVYEGVYTNHKGEKINVAVKTCKKDCTLDNKEKFMSEAVIMKNLDHPHIVKLI
  GIIEEEPTWIVMELYPYGELGHYLERNKNSLKVPTLVLYALQICKAMAYLESINCVHRDIAVRNILVASP
  ECVKLGDFGLSRYIEDEDYYKASVTRLPIKWMSPESINFRRFTTASDVWMFAVCMWEILSFGKQPFFWLE
  NKDVIGVLEKGDRLPKPELCPPVLYTLMTRCWDYDPSDRPRFTELVCSLSDIYQMERDIAIEQERNARYR
  PPKILEPTAFQEPPPKPSRPKYKHPPQTNLLAPKLQFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHR
  HNVFKRHSMREEDFIRPSSREEAQQLWEAEKIKMRQVLDRQQKQMVEDSQWLRREERCLDPMVYMNDKSP
  LTPEKEAGYTEFTGPPQKPPRLGAQSIQPTANLDRTDDLVYHNVMTLVEAVLELKNKLSQLPPEEYVVVV
  KNVGLNLRKLIGSVDDLLPSLPASSRTEIEGTQKLLNKDLAELINKMRLAQQNAVTSLSEDCKRQMLTAS
  HTLAVDAKNLLDAVDQAKVVANLAHPPAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017318
• ORF Size: 3027 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017318.2, NP_059014.2
• RefSeq Size: 3907 bp
• RefSeq ORF: 3030 bp
• Locus ID: 50646
• UniProt ID: P70600
• Cytogenetics: 15p12
• MW: 115.8 kDa
• Gene Summary: may phosphorylate focal adhesion kinase FAK; may play a role in actin cytoskeleton reorganization; may mediate thrombin induced astrocyte proliferation [RGD, Feb 2006]