Mst1 (NM_024352) Rat Tagged ORF Clone

CAT#: RR207116

Mst1 (Myc-DDK-tagged ORF) - Rat Macrophage stimulating 1 (hepatocyte growth factor-like) (Mst1), (10 ug)

Product Images

Circular map for RR207116

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mst1
• Synonyms: E2F2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207116 representing NM_024352
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTGGCTCCCACTACTGCTGCTTCTGGCACAGTGTTCAAGGGCTCTTGGGCAGCGCTCACCGCTGA
  ATGACTTCCAGCTGCTTCGGGGCACAGAGTTAAGGAACCTGCTACATCCAGTGGTGCCAGGGCCATGGCA
  GGAGGATGTGGCAGATGCCGAGGAGTGTGCTAGACGCTGTGGGCCCCTTCTGGACTGCCGAGCCTTCCAC
  TACAATATGAGCAGCCATGGTTGCCAGCTACTACCGTGGACTCAGCACTCTCTGCGTGCACAGCTACACC
  ATTCTAGCCTGTGCGATCTCTTCCAGAAGAAAGACTATGTACGGACCTGCATTATGGACAATGGGGCCAG
  CTACCGGGGCACTGTGGCCAGGACAGCTGATGGCTTGCCCTGCCAAGCCTGGAGCCGCAGGTTCCCCAAT
  GACCACAAGTACACGCCCACACCGAAGAATGGCCTGGAAGAGAACTTCTGTCGGAACCCTGATGGGGACC
  CCAGAGGTCCCTGGTGCTACACGACAAACCGCAGCGTGCGTTTCCAGAGCTGCGGCATCAAATCATGCAG
  GGAGGCGGTTTGTGTTTGGTGCAACGGCGAGGATTACCGTGGCGAGGTAGACGTTACAGAATCGGGACGG
  GAGTGTCAACGCTGGGACCTGCAGCACCCACACTCGCACCCTTTCCACCCTGAAAAGTTCCCAGACAAAG
  CTCTGAAAGACAACTATTGCCGTAATCCGGATGCATCTGAGCGCCCCTGGTGCTACACCACGGACCCGAA
  TGTTGAGCGAGAGTTCTGTGACCTGCCCAGTTGCGGGCCCAACCTGCCACCGACCACCAAAGGATCCAAG
  TCACAACAGCGCAACAAGGTCAAGGCTTCGAACTGCTTCCGCGGAAAAGGTGAAGACTATCGAGGCACAA
  CCAATACCACCTCTGCGGGTGTGCCCTGCCAGCGCTGGGATGCGCAGAATCCGCACCAGCACCGCTTTGT
  GCCGGAGAAATATGCTTGCAAGGACCTTCGTGAGAATTTCTGCCGGAATCCTGATGGCTCCGAGGCGCCT
  TGGTGCTTCACATCTCGACCTGGTTTGCGTGTGGCCTTTTGCTACCAGATCCCACGCTGCACAGAAGAAG
  TGGTGCCAGAGGGCTGCTACCATGGCTCAGGTGAACAGTATCGTGGCTCAGTCAGCAAGACACGCAAGGG
  CGTTCAGTGCCAGCACTGGTCCTCAGAGACACCACATAAGCCACAATTCACACCCACCTCAGCACCACAT
  GCAGGCTTGGAGGCAAACTTTTGCCGGAATCCGGATGGAGATAGCCATGGGCCCTGGTGTTATACTTTGG
  ACCCAGAGACCCTGTTTGACTACTGTGCCCTAAAACGCTGTGATGATGACCAGCCACCATCCATCTTGGA
  CCCCCCAGTCCAGGTGCAGTTTGAAAAGTGTGGCAAGAGAGTTGACCAGAGTAATAGACTTCGTGTGGTG
  GGGGGTCATCCTGGGAACTCACCGTGGACAGTCAGCTTGCGGAATCGACAGGGCCAGCATTTCTGTGGGG
  GTTCCCTAGTGAAGGAGCAGTGGGTACTGACCGCCCGGCAATGCATCTGGTCATGCCATGATCCTCTCAC
  AGGATATGAGGTATGGTTGGGTACAATTAACCAGAACCCACAGCCTGGAGAAGCAAACCTGCAGAGGGTC
  TCAGTGGCCAAGACAGTGTGCGGACCTGCAGGCTCCCAACTTGTTCTGCTCAAGCTGGAGAGACCTGTGA
  TCCTGAACCATCACGTGGCCAGGATTTGCCTACCTCCTGAACAGTATGTGGTACCTCCAGGGACCAACTG
  CGAGATCGCTGGCTGGGGTGAATCCAAAGGTACAAGCAATAGCACAGTCCTTCATGTGGCCAAAATGAAG
  GTCATCTCCAGTCAGGAATGTAATGTGAAGTACCGGAGACGAGTACAAGAGAGTGAGATATGCACCGAGG
  GGTTGCTGGCCCCTACGGGCGCTTGTGAGGGTGACTACGGGGGCCCACTTGCCTGCTATACCCATGACTG
  CTGGGTCCTACAGGGACTTATCATCCCGAACAGAGTGTGTGCACGGCCTCGCTGGCCAGCTATCTTCACA
  CGTGTGTCTGTGTTTGTGGACTGGATTAACAAGGTCGTGCAGCTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207116 representing NM_024352
  Red=Cloning site Green=Tags(s)
  
  MGWLPLLLLLAQCSRALGQRSPLNDFQLLRGTELRNLLHPVVPGPWQEDVADAEECARRCGPLLDCRAFH
  YNMSSHGCQLLPWTQHSLRAQLHHSSLCDLFQKKDYVRTCIMDNGASYRGTVARTADGLPCQAWSRRFPN
  DHKYTPTPKNGLEENFCRNPDGDPRGPWCYTTNRSVRFQSCGIKSCREAVCVWCNGEDYRGEVDVTESGR
  ECQRWDLQHPHSHPFHPEKFPDKALKDNYCRNPDASERPWCYTTDPNVEREFCDLPSCGPNLPPTTKGSK
  SQQRNKVKASNCFRGKGEDYRGTTNTTSAGVPCQRWDAQNPHQHRFVPEKYACKDLRENFCRNPDGSEAP
  WCFTSRPGLRVAFCYQIPRCTEEVVPEGCYHGSGEQYRGSVSKTRKGVQCQHWSSETPHKPQFTPTSAPH
  AGLEANFCRNPDGDSHGPWCYTLDPETLFDYCALKRCDDDQPPSILDPPVQVQFEKCGKRVDQSNRLRVV
  GGHPGNSPWTVSLRNRQGQHFCGGSLVKEQWVLTARQCIWSCHDPLTGYEVWLGTINQNPQPGEANLQRV
  SVAKTVCGPAGSQLVLLKLERPVILNHHVARICLPPEQYVVPPGTNCEIAGWGESKGTSNSTVLHVAKMK
  VISSQECNVKYRRRVQESEICTEGLLAPTGACEGDYGGPLACYTHDCWVLQGLIIPNRVCARPRWPAIFT
  RVSVFVDWINKVVQLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024352
• ORF Size: 2148 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024352.1, NP_077328.1
• RefSeq Size: 2222 bp
• RefSeq ORF: 2151 bp
• Locus ID: 24566
• Cytogenetics: 8q32
• MW: 80.7 kDa
• Gene Summary: ligand for receptor tyrosine kinase STK/RON; may mediate germ cell-germ cell interaction during spermatogenesis, and acquisition of sperm motility and/or fertilizing capacity in the epididymis [RGD, Feb 2006]